The anti‐ageing potential of a new jasmonic acid derivative (LR2412): in vitro evaluation using reconstructed epidermis episkin™

Abstract

Jasmonic acid is involved in plant wound repair and tissue regeneration, but no study has been reported in human skin. The effect of a jasmonic acid derivative, tetra-hydrojasmonic acid (LR2412, 1 and 10 lm) was investigated on an in vitro reconstructed skin model, Episkin TM . Using real time RTQPCR studies, results showed an increase in hyaluronan synthase 2 (HAS2) and hyaluronase synthase 3 (HAS3) expression. Furthermore, an increase in hyaluronic acid (HA) deposits in basal and suprabasal layers of the epidermis was observed. The percentage of positive Ki67 keratinocytes in the basal layer as well as the epidermis thickness were seen to increase. Immunohistochemistry studies showed that the synthesis of late differentiation proteins filaggrin and transglutaminase 1 was not modified. The human epidermis is known to thin with age while HA content has been reported to decrease. These results illustrate the potential of LR2412 in counteracting signs of skin ageing.

Over-the-counter anti-ageing topical agents and their ability to protect and repair photoaged skin

“…The same requirement is true for botanical extracts, as efficacy data are often limited to just one or two studies, many of which were conducted in vitro and therefore may not relate to an in vivo environment. Despite these limitations, the available data suggest that some botanical extracts may act as topical interventions for photoageing [86][87][88].…”

“…However the in vivo efficacy and composition of many of these formulations have yet to be scientifically validated. Certain extracts are suggested to exert a preventative effect through antioxidant activity [82] whilst others have been implicated in dermal matrix repair through MMP inhibition or deposition of ECM components [83][84][85][86][87][88][89][90][91][92][93] (Table 2). For many of these botanical extracts, few studies have been published, little is known of their potential mechanisms of action and problems with batch to batch consistency plague attempts to define their molecular compositions.…”

“…Potential or proven benefits to photoaged skin Increased synthesis and deposition of HA in a skin equivalent model [87]. In vivo, induced fibrillin-rich-microfibrils deposition at the DEJ after 12 days topical application under occluded patches; 3 months daily application to face lead to reduction in wrinkle length and total wrinkle surface in crow's feet area [88] Panax ginseng extracts Found to reduce UVB-induced skin wrinkling and loss of elasticity when applied topically in a murine model [89] and to induce collagen I deposition and reduce MMP-I in HDFs in vitro [96,97] Punica granatum (pomegranate) extracts A peel extract stimulated procollagen I synthesis and inhibited MMP-1 in HDFs in vitro [92].…”

The Potential Use of Marine Microalgae and Cyanobacteria in Cosmetics and Thalassotherapy

“…Although the functional role of microalgal endogenous phytohormones remains elusive, molecular evidence from the oleaginous microalgae Nannochloropsis oceanica suggests that endogenous abscisic acid and cytokinin are functional, and that their physiological effects are similar to those in higher plants [49]. That means the microalgal phytohormones could play a role in counteracting signs of skin ageing [50], a promising future in skin care cosmetics.…”

Defense Induced by Jasmonic Acid: A Review

“…Therefore, itwas concluded that the JAs control tuber formation indirectly through crosstalk with GA signaling (Wasternack and Hause, 2013). Recently Michelet et al, 2012 found that one of the derivatives of the jasmonic acid, namely tetra-hydro jasmonic acid (LR2412) has shown anti-aging potential in case of human skin. LR2412 is known to increase the expression of hyaluronase synthase 2 (HAS2) and hyaluronase synthase 3 (HAS3) which consequently increase the synthesis of hyaluronic acid (HA) Plants are associated with diverse microbial communities that influence their health and nutrition.…”