The anti-angiogenic herbal extract from Melissa officinalis inhibits adipogenesis in 3T3-L1 adipocytes and suppresses adipocyte hypertrophy in high fat diet-induced obese C57BL/6J mice

Woo, Sangee; Yoon, Miso; Kim, Jeongjun; Hong, Yeonhee; Kim, Minyoung; Shin, Soon Shik; Yoon, Michung

doi:10.1016/j.jep.2015.12.015

Cited by 22 publications

(16 citation statements)

References 38 publications

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“…It has also been reported that anti-angiogenic therapy provides a possible therapeutic choice for the treatment of diet-induced obesity and metabolic complications. 47–51 Given this, it is convinced that this novel angiogenic inhibitor will have wide application prospects.…”

Section: Discussionmentioning

confidence: 99%

A novel peptide specifically binding to VEGF receptor suppresses angiogenesis in vitro and in vivo

Zhang

Liu

et al. 2017

Sig Transduct Target Ther

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Vascular endothelial growth factor (VEGF), one of the most important angiogenic factors, plays an essential role in both physiological and pathological angiogenesis through binding to VEGF receptors (VEGFRs). Here we report a novel peptide designated HRHTKQRHTALH (peptide HRH), which was isolated from the Ph.D. -12 phage display library using VEGFR-Fc fusion protein as the bait. This peptide was found to dose-dependently inhibit the proliferation of human umbilical vein endothelial cells stimulated by VEGF. The anti-angiogenesis effect of the HRH peptide was further confirmed in vivo using the chick chorioallantoic membrane assay, which was also dose-dependent. Besides, peptide HRH was proved to inhibit corneal neovascularization in an alkali-burnt rat corneal model and a suture-induced rat corneal model. Taken together, these findings suggest that the HRH peptide can inhibit angiogenesis both in vitro and in vivo. Consequently, the HRHTKQRHTALH peptide might be a promising lead peptide for the development of potential angiogenic inhibitors.

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Section: Discussionmentioning

confidence: 99%

A novel peptide specifically binding to VEGF receptor suppresses angiogenesis in vitro and in vivo

Zhang

Liu

et al. 2017

Sig Transduct Target Ther

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“…A voucher specimen of Melissa officinalis (AL-20121015-001-R) was deposited at the AngioLab Inc. (Daejeon, Korea). ALS-L1023 was standardized with two reference compounds of rosmarinic acid and caffeic acid by high-performance liquid chromatography [22], and then dissolved in 100% dimethyl sulfoxide and used for in vitro tests.…”

Section: Methodsmentioning

confidence: 99%

“…We previously found that an active fraction, denominated ALS-L1023, of an organic extraction from lemon balm ( Melissa officinalis L.) leaves exhibited anti-angiogenic activities [21,22]. Here, we examined the effects of ALS-L1023 on angiogenesis, visceral obesity, and obesity-induced NAFLD in HFD-fed C57BL/6J mice and investigated its mechanism of action.…”

Section: Introductionmentioning

confidence: 99%

The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function

Kim

Lee

Lim

et al. 2017

IJMS

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Similar to neoplastic tissues, growth and development of adipose tissue are thought to be angiogenesis-dependent. Since visceral adipose tissue (VAT) is associated with development and progression of nonalcoholic fatty liver disease (NAFLD), we hypothesized that angiogenesis inhibition would attenuate obesity-induced NAFLD. We fed C57BL/6J mice a low-fat diet (LFD, chow 10% kcal fat), a high-fat diet (HFD, 45% kcal fat) or HFD supplemented with the lemon-balm extract ALS-L1023 (HFD-ALS) for 15 weeks. ALS-L1023 reduced endothelial cell-tube formation in vitro. HFD increased VAT angiogenesis and induced weight gains including body weight, VAT mass and visceral adipocyte size compared with LFD. However, HFD-ALS led to weight reductions without affecting calorie intake compared with HFD. HFD-ALS also reduced serum ALT and AST levels and improved lipid metabolism. HFD-ALS suppressed steatosis, infiltration of inflammatory cells, and accumulation of collagen in livers. HFD-ALS modulated hepatic expression of genes involved in lipid metabolism, inflammation, fibrosis, antioxidation, and apoptosis. Concomitantly, analysis of VAT function revealed that HFD-ALS led to fewer CD68-positive macrophage numbers and lower expression of inflammatory cytokines compared with HFD. Our findings show that the anti-angiogenic herbal extract ALS-L1023 attenuates NAFLD by targeting VAT during obesity, suggesting that angiogenesis inhibitors could aid in the treatment and prevention of obesity-induced human NAFLD.

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“…Lemon balm extract, ALS-L1023, also regulates adipogenesis and obesity by inhibiting angiogenesis and MMP activities in animal models of obesity [ 93 , 155 , 156 ]. ALS-L1023 inhibits endothelial cell VEGF- and FGF-induced tube formation [ 155 ]. Its inhibitory effect on endothelial cell proliferation is comparable to that of TNP-470.…”

Section: Antiobesity Effects Of Antiangiogenic Herbal Medicinesmentioning

confidence: 99%

Regulation of Obesity by Antiangiogenic Herbal Medicines

Shin

Yoon

2020

Molecules

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Obesity is the result of an energy imbalance caused by an increased ratio of caloric intake to energy expenditure. In conjunction with obesity, related metabolic disorders, such as dyslipidemia, atherosclerosis, and type 2 diabetes, have become global health problems. Obesity progression is thought to be associated with angiogenesis and extracellular matrix (ECM) remodeling. Angiogenesis occurs in growing adult adipose tissues, which are similar to neoplastic tissues. Adipose tissue is highly vascularized, and each adipocyte is nourished by an extensive capillary network. Adipocytes produce proangiogenic factors, such as vascular endothelial growth factor A and fibroblast growth factor 2, which promote neovascularization within the adipose tissue. Furthermore, matrix metalloproteinases (MMPs), including MMP-2 and MMP-9, play important roles in adipose tissue development and microvessel maturation by modifying the ECM. Thus, modulation of angiogenesis and MMP activity provides a promising therapeutic approach for controlling human obesity and its related disorders. Over the past decade, there has been a great increase in the use of alternative treatments, such as herbal remedies, for these diseases. This review will focus on the role of angiogenesis in adipose tissue growth and the regulation of obesity by antiangiogenic herbal medicines.

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The anti-angiogenic herbal extract from Melissa officinalis inhibits adipogenesis in 3T3-L1 adipocytes and suppresses adipocyte hypertrophy in high fat diet-induced obese C57BL/6J mice

Cited by 22 publications

References 38 publications

A novel peptide specifically binding to VEGF receptor suppresses angiogenesis in vitro and in vivo

A novel peptide specifically binding to VEGF receptor suppresses angiogenesis in vitro and in vivo

The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease through Regulating the Visceral Adipose-Tissue Function

Regulation of Obesity by Antiangiogenic Herbal Medicines

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