The anti-idiotypic antibody 1F7 stimulates monocyte interleukin-10 production and induces endotoxin tolerance

Davtyan, Tigran K.; Poghosyan, David; Sukiasyan, Anna G; Grant, Michael D.

doi:10.1186/1476-9255-10-14

Cited by 3 publications

(1 citation statement)

References 40 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…AId is employed as a vaccine, as potential serodiagnosis reagents in immunoassays and substations of highly toxic substances (Deyev & Lebedenko, 2009;Hu et al, 2017;Koprowski, Herlyn, Lubeck, DeFreitas, & Sears, 1984;Ruffini et al, 2014). In recent years, anti-idiotypic antibody technology has been used to prepare vaccines for hepatitis A, B, C and various diseases (Davtyan, Poghosyan, Sukiasyan, & Grant, 2013;Grant, 2002;Kiyohara et al, 2009;Yildirim, Başalp, Yücel, Manav, & Sezen, 2004). Eger et al (2016) also reported that a new human/mouse chimeric anti-idiotype Ab ganglidiximab may have the potential to tailor immune responses to the paratope regions mimicking GD2 overexpressed in neuroblastoma (NB) as well.…”

Section: Introductionmentioning

confidence: 99%

Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Hao

Wang

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

Anti-idiotypic antibody technology has been widely developed. Here, we prepared the F(ab') 2 fragments of the Cry1F toxin polyclonal antibody as Ab1and screened an anti-idiotype nanobody (VHH) for Bt Cry1F toxin from the camelid naive antibody phage display library. After three cycles of panning, six clones were isolated and subjected to indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for the detection of the Cry1F toxin. A competition inhibition rate of 47.00% exists between the anti-idiotype nanobody with the highest activity (5B) and Cry1F toxin for F(ab') 2 fragments. The binding abilities between the anti-idiotypic nanobody 5B and brush border membrane vesicles (BBMV) of Ostrinia furnacalis (Guenée) are also good. The anti-idiotypic nanobody 5B expressed through the prokaryotic system has the same activity as in phage form, and mortality compared to controls could reach 23.3% (P < 0.05). The material has the potential to simulate the Cry1F toxin to a certain extent.

show abstract

Section: Introductionmentioning

confidence: 99%

Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Hao

Wang

et al. 2019

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance

Zhao

Tao

et al. 2023

Inflamm. Res.

View full text Add to dashboard Cite

Background Endotoxin tolerance (ET) is a protective mechanism in the process of sepsis, septic shock, and their sequelae including uncontrolled inflammation. Accumulating evidence has shown that peripheral T cells contribute to the induction of ET. However, what and how T-cell development contributes to ET inductions remain unclear. Methods Mice were intraperitoneally injected with LPS at a concentration of 5 mg/kg to establish an LPS tolerance model and were divided into two groups: a group examined 72 h after LPS injection (72-h group) and a group examined 8 days after LPS injection (8-day group). Injection of PBS was used as a control. We performed high-throughput sequencing to analyze the characteristics and changes of CD4+SP TCRβ CDR3 repertoires with respect to V direct to J rearrangement during the ET induction. Moreover, the proportion and proliferation, as well as surface molecules such as CD80 and CD86, of F4/80+ macrophages were analyzed using FCM. Furthermore, ACT assay was designed and administered by the tail vein into murine LPS-induced mouse model to evaluate the role of F4/80+ macrophages on the development of CD4+SP thymocytes in ET condition. Results We found that the frequency and characteristics of the TCRβ chain CDR3 changed obviously under condition of ET, indicating the occurrence of TCR rearrangement and thymocyte diversification. Moreover, the absolute numbers of F4/80+ macrophages, but not other APCs, were increased in thymic medulla at 72-h group, accompanied by the elevated function-related molecules of F4/80+ macrophages. Furthermore, adoptively transferred OVA332-339 peptide-loaded macrophages into Rag-1−/− mice induced the clone deletion of OVA-specific CD4+SP, thereby ameliorating the pathology in lung tissue in LPS challenge. Conclusions These data reveal that the frequency and characteristics of the TCRβ chain CDR3 undergo dynamic programming under conditions of LPS tolerance. Furthermore, the peripheral macrophages may be a key factor which carry peripheral antigen to thymic medulla and affect the negative selection of T-cell population, thereby contributing to the formation of ET. These results suggest that the clone selection in thymus in ET may confer protection against microbial sepsis.

show abstract

What Can We Learn from Research with Monoclonal Antibody 1F7?

Köhler

2022

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

View full text Add to dashboard Cite

The anti-idiotypic antibody 1F7 stimulates monocyte interleukin-10 production and induces endotoxin tolerance

Cited by 3 publications

References 40 publications

Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

Screening and activity identification of an anti-idiotype nanobody for Bt Cry1F toxin from the camelid naive antibody phage display library

TCR repertoire landscape reveals macrophage-mediated clone deletion in endotoxin tolerance

What Can We Learn from Research with Monoclonal Antibody 1F7?

Contact Info

Product

Resources

About