The Anti-Inflammatory Effect of Paeoniflorin on Cerebral Infarction Induced by Ischemia-Reperfusion Injury in Sprague-Dawley Rats

Abstract

Paeoniflorin, a component in Paeonia lactiflora Pall, inhibits nuclear factor-kappaB expression in chronic hypoperfusion rat and has anti-inflammatory properties. Therefore, the aim of the present study was to investigate the effect of paeoniflorin on cerebral infarct, and the involvement of anti-inflammation. We established an animal model of cerebral infarct by occluding both the common carotid arteries and the right middle cerebral artery for 90 min, followed by reperfusion of 24 hours. The ratios of cerebral infarction area to total brain area, and neuro-deficit score were used as an index to observe the effects of paeoniflorin on cerebral infarct. ED1 (mouse anti rat CD68), interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), intercellular adhesion molecular-1 (ICAM-1), myeloperoxidase (MPO) immunostaining and apoptotic cells in the cerebral infarction region also were studied. The results indicated that both pre-treatment and post-treatment with paeoniflorin reduced the ratio of cerebral infarction area; pre-treatment with paeoniflorin also reduced the neurological deficit score. The counts of ED1, IL-1beta, TNF-alpha, ICAM-1 of microvessels and MPO immunoreactive cells and apoptotic cells were increased in the cerebral infarction region; however, these increases were reduced by Paeoniflorin pre-treatment. In conclusion, Paeoniflorin reduced cerebral infarct and neurological deficit in ischemia-reperfusion injured rats, suggesting that paeoniflorin may have a similar effect in humans and might be a suitable treatment for stroke. Paeoniflorin reduced cerebral infarct, at least in part, involves the anti-inflammatory properties.

Sesamol attenuates oxidative stress, apoptosis and inflammation in focal cerebral ischemia/reperfusion injury

“…In the present study, MCAO rats exhibited an upregulation in TNF-α and IL-1β mRNA expression in ischemic brain tissue. These results are corroborated by previous studies, which reported that exacerbated TNF-α and IL-1β levels may be important factors in reperfusion injury following transient brain ischemia (51); however, sesamol treatment significantly downregulated the expression of proinflammatory cytokines via its anti-inflammatory activity (52,53).…”

Morin Mitigates Oxidative Stress, Apoptosis and Inflammation in Cerebral Ischemic Rats

“…In our study MCAO rats displayed upregulation of TNF-α and IL-1β mRNA expression in ischemic brain tissue. The reports highlighted in the present study are in corroboration with the previous reports that aggravated TNF-α and IL-1β levels are one of the cardinal factors in brain damage after transient brain ischemia (Tang et al, 2010). However, Morin intervention effectively downregulated the expression of proinflammatory cytokines mediated through its anti-inflammatory activity (Fang et Furthermore, Caspase-3, is the key protein involved in the apoptosis, which is highly vital for DNA damage and the noxious cell morphology alteration in relation to apoptosis (Cohen, 1997;Janicke et al, 1998).…”

Paeoniflorin attenuates hippocampal damage in a rat model of vascular dementia

“…1) is the principal bioactive component of Paeoniae radix which has been used for >1,000 years in traditional Chinese medicine (11). In modern pharmacological studies, PF exhibits numerous pharmacological effects such as anti-inf lammation, antioxidant, endothelium-dependent vasorelaxation, neuromuscular blocking and cognition enhancement properties (12,13). Xiao et al (14) demonstrated that PF is able to reverse, or alleviate, impairments.…”