The anti-inflammatory effects of Caragana tangutica ethyl acetate extract

Niu, Xiaofeng; Li, Yongmei; Liu, Weifeng; Hu, Hua; Yao, Huan; Li, Huani; Mu, Qingli

doi:10.1016/j.jep.2013.12.026

Cited by 26 publications

(12 citation statements)

References 22 publications

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“…The carrageenan-induced paw edema is a useful model to investigate the anti-inflammatory effect of plant extracts and natural products [26]. Carrageenan- induced inflammation consists of two phases [27]: the early phase (first 2 h after carrageenan injection) is attributed to the release of pro-inflammatory mediators, such as histamine and serotonin [28], and the second phase (3-5 h after carrageenan injection) is mainly mediated by kinins, prostaglandin, nitric oxide, cyclooxygenase [28], cytokines, and neutrophil-derived free radicals [29]. In the present study, we observed that GL caused significant reduction of edema in the both phases, revealing that its anti-inflammatory effect is most likely due to the inhibition of different aspects and mediators of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Observing Anti-inflammatory and Anti-nociceptive Activities of Glycyrrhizin Through Regulating COX-2 and Pro-inflammatory Cytokines Expressions in Mice

Wang

Niu

et al. 2015

Inflammation

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The present study aimed to investigate the potential anti-inflammatory and anti-nociceptive activities of glycyrrhizin (GL) in mice and to explore the possible related mechanisms. Xylene-induced ear edema, carrageenan-induced paw edema and acetic acid-induced vascular permeability test were used to investigate the anti-inflammatory activities of GL in mice. Anti-nociceptive effects of GL were assessed by using acetic acid-induced writhing, hot plate test and formalin test, as well as evaluation of spontaneous locomotor activity and motor performance. The mRNA expression of pro-inflammatory cytokines (such as TNF-α, IL-6 and iNOS) and the protein expression of cyclooxygenase-2 (COX-2) were explored by using real-time fluorogenic PCR and Western blot, respectively. The results showed that GL significantly reduced xylene-induced ear edema, carrageenan-induced paw edema, and acetic acid-induced vascular permeation. Additionally, GL significantly inhibited the nociceptions induced by acetic acid and formalin. However, the nociceptions could not be decreased by GL in the hot plate test, and GL did not affect spontaneous locomotor activity and motor performance. The expression levels of TNF-α, IL-6, iNOS and COX-2 were significantly downregulated by GL. In conclusion, GL exerts significant anti-inflammatory and analgesic activities by attenuating the expression levels of TNF-α, IL-6, iNOS and COX-2.

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Section: Discussionmentioning

confidence: 99%

Observing Anti-inflammatory and Anti-nociceptive Activities of Glycyrrhizin Through Regulating COX-2 and Pro-inflammatory Cytokines Expressions in Mice

Wang

Niu

et al. 2015

Inflammation

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“…Writhing test also bounces positive results with sedatives, muscle relaxants, and many other pharmacological activities which question its sensitivity and specificity. Intraperitoneal administration of acetic acid involved local peritoneal receptors and are mediated by indirect release endogenous nociceptive mediators, prostaglandins [ 27 ], and sympathomimetic system mediators like PGE2 and PGF2 α that stimulate the nonsteroidal anti-inflammatory drugs (NSAIDs) and opioids-sensitive nociceptive neurons. Acetic acid also persuades sympathetic nervous system mediators that achieve peak level at first 30 min after acetic acid injection.…”

Section: Resultsmentioning

confidence: 99%

Antinociceptive and Anti-Inflammatory Activities ofBridelia retusaMethanolic Fruit Extract in Experimental Animals

Kumar

Jain

2014

The Scientific World Journal

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Antinociceptive and anti-inflammatory potentials of methanolic extract of Bridelia retusa fruit (BRME) were evaluated against different animal models in rodents. Antinociceptive effects of BRME were assessed in mice using the acetic acid-induced writhing and formalin test. Anti-inflammatory effects of BRME in three different doses, namely, 100, 200, and 400 mg/kg, were evaluated by utilizing different animal models representing various changes associated with inflammation, namely, carrageenan-induced paw oedema, histamine and serotonin-induced paw oedema, arachidonic acid-induced paw oedema, formalin-induced paw oedema, TPA-induced ear oedema, acetic acid-induced vascular permeability, total WBC count in paw fluid, and myeloperoxidase assay. Also BRME was phytochemically evaluated using chromatographic method. The BRME did not exhibit any signs of toxicity up to a dose of 2000 mg/kg. The extract showed statistical significant inhibition of induced nociception and inflammation in dose dependent manner. The higher dose of extract significantly inhibited pain and inflammation against control (P < 0.001). HPLC results revealed the presence of gallic acid and ellagic acid as phytoconstituents in BRME and it was proven as anti-inflammatory agents. The present study scientifically demonstrated the antinociceptive and anti-inflammatory potential of fruit of B. retusa methanolic extract. These effects may be attributed to the presence of polyphenolic phytoconstituents in the extract.

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“…The method used for this investigation was similar to that reported previously [ 11 ]. Forty male SD rats were divided into four groups: model control (saline); alkaloid extract of CB (30 mg/kg and 60 mg/kg); aspirin (400 mg/kg).…”

Section: Methodsmentioning

confidence: 93%

“…The xylene-induced ear edema test with modifications was conducted as described previously [ 11 ]. Briefly, four groups of ten mice were treated (via the oral route) with physiological (0.9 %) saline (control), alkaloid extract of CB (40 and 80 mg/kg) or aspirin (150 mg/kg; positive control).…”

Section: Methodsmentioning

confidence: 99%

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

Dong

Zhang

Zhao

et al. 2015

BMC Complement Altern Med

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BackgroundCorydalis bungeana Turcz. (CB; family: Corydalis DC.) is an anti-inflammatory medicinal herb used widely in traditional Chinese medicine (TCM) for upper respiratory tract infection, etc., but its anti-inflammatory active molecules are unknown. This study was designed to screen for the anti-inflammatory components from CB based on macrophage binding combined with HPLC.MethodsXylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats were used to evaluate the anti-inflammatory activity of CB. The macrophage binding with high-performance liquid chromatography (HPLC) analysis and HPLC-MS were established to screen the potential active compounds. ELISA kits were performed to measure the levels of IL-6, IL-10, TNF-α and NO in RAW 264.7 macrophages culture media.ResultsThe alkaloid extract of CB could inhibit significantly xylene-induced ear edema in mouse and carrageenan-induced hind-paw edema in rats. Two components binded to RAW 264.7 cell were identified as 12-hydroxycorynoline and corynoline. Bioassays demonstrated that these two compounds significantly inhibited LPS-induced IL-6, IL-10, TNF-α and NO levels.ConclusionsThe results suggest that corynoline and 12-hydroxycorynoline contribute to the anti-inflammatory effects of the alkaloid extract of CB. Our findings suggest that these two compounds can be used as candidate for anti-inflammatory drugs.

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The anti-inflammatory effects of Caragana tangutica ethyl acetate extract

Cited by 26 publications

References 22 publications

Observing Anti-inflammatory and Anti-nociceptive Activities of Glycyrrhizin Through Regulating COX-2 and Pro-inflammatory Cytokines Expressions in Mice

Observing Anti-inflammatory and Anti-nociceptive Activities of Glycyrrhizin Through Regulating COX-2 and Pro-inflammatory Cytokines Expressions in Mice

Antinociceptive and Anti-Inflammatory Activities ofBridelia retusaMethanolic Fruit Extract in Experimental Animals

Screening for anti-inflammatory components from Corydalis bungeana Turcz. based on macrophage binding combined with HPLC

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