The Anti-proliferative Effects of Recombinant Human Lysozyme on Human Gastric Cancer Cells

Abstract

We investigated the anti-proliferative effects of recombinant human lysozyme (rHlys) on gastric cancer cell lines and normal human lung fibroblasts. Using conventional molecular cloning techniques we purified rHlys, which we incubated with cultured cells and measured the effects on cell proliferation and viability. At concentrations of 100 and 1000 microg/l, rHlys significantly inhibited the growth of human gastric cancer cell lines. In contrast, 10 and 50 microg/l of rHlys stimulated gastric cancer cell growth. None of the concentrations of rHlys affected cell viability. Only the highest concentration of rHlys (1000 microg/l) inhibited human lung fibroblast growth. Our results suggest that 100 microg/l is the optimum growth inhibiting concentration, which inhibited cancer cell growth but not normal cell growth. Our in vitro findings suggest that genetically engineered rHlys might inhibit human gastric cancer cell proliferation in vivo, so it might warrant further investigation as a potential novel anti-cancer agent.

Magnetic molecularly imprinted nanoparticles for recognition of lysozyme

“…Its common applications are as an important index in the diagnosis of various diseases including renal disease and leukemia. It also can be used as a cell disrupting agent in ophthalmologic preparations, as a food additive in milk products and as a drug for the treatment of ulcer and infections (Guo et al, 2007;Zheng et al, 2007). Furthermore, it has been demonstrated by animal experiment and in vitro cell chemotherapy that lysozyme can be used as a drug in the treatment of HIV infection (Siddhartha and Rein, 2009;Heriazon et al, 2009).…”

Identification of CEA-interacting proteins in colon cancer cells and their changes in expression after irradiation

“…The expression of lysozyme was positively correlated with a favorable outcome in breast cancer [ 20 ] and was upregulated to a greater extent in mucinous cell carcinomas than in well-differentiated and poorly differentiated gastric adenocarcinomas [ 21 ]. Lysozyme directly activates immune cells and increases tumor cell immunogenicity [ 22 ], which have been confirmed in a number of experimental cases [ 22 - 24 ]. However, even without immune cell activation, self-assembled nanostructured hen egg white lysozyme and recombinant human lysozyme showed strong antiproliferative effects in breast [ 23 ] and gastric [ 24 ] cancer cells, respectively.…”

“…Lysozyme directly activates immune cells and increases tumor cell immunogenicity [ 22 ], which have been confirmed in a number of experimental cases [ 22 - 24 ]. However, even without immune cell activation, self-assembled nanostructured hen egg white lysozyme and recombinant human lysozyme showed strong antiproliferative effects in breast [ 23 ] and gastric [ 24 ] cancer cells, respectively. However, a better understanding of the antitumor mechanisms of lysozyme (intracellular or extracellular) is needed.…”

Sulfanilic acid‐modified chitosan mini‐spheres and their application for lysozyme purification from egg white

“…This enzyme is commercially interesting mainly due to its antimicrobial activity, because it damages bacterial cell walls by catalyzing the hydrolysis of 1,4‐β‐bonds between GlcNAc and muramic acid . This enzyme has several applications in pharmaceutical, food and veterinary industries as fungistatic, anti‐inflammatory, antitumor, antiviral, a natural food preservative, etc . Therefore, the search for efficient and scalable strategies for its purification involves both an academic and an industrial challenge.…”

“…11,12 This enzyme has several applications in pharmaceutical, food and veterinary industries as fungistatic, anti-inflammatory, antitumor, antiviral, a natural food preservative, etc. [13][14][15] Therefore, the search for efficient and scalable strategies for its purification involves both an academic and an industrial challenge. Many purification methods such as precipitation, ultrafiltration and liquid chromatography, have been developed.…”