The Antibacterial Activity and Mechanism of Chlorogenic Acid Against Foodborne Pathogen <i>Pseudomonas aeruginosa</i>

Su, Mengmeng; Liu, Fang; Luo, Zhijun; Wu, Haixia; Zhang, Xinxiao; Wang, Daoying; Zhu, Yun; Sun, Zhilan; Xu, Weimin; Ying, Miao Miao

doi:10.1089/fpd.2019.2678

Cited by 61 publications

(40 citation statements)

References 30 publications

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“…The antibacterial effects of quercetin have been demonstrated in several studies ( 38 , 39 ), and quercetin has even been found to be effective in restoring the intestinal microbiota of mice after antibiotic treatment ( 40 ). Meanwhile, chlorogenic acid was found to have antibacterial activity against Foodborne Pathogen Pseudomonas aeruginosa and Salmonella enteritidis ( 41 ), and caffeic acid was found to have antibacterial activity against Staphylococcus aureus clinical strains ( 42 ). In addition, the antibacterial effect of Salvia miltiorrhiza Bunge (Danshen), a major component herb of HQT, has been widely reported and studied ( 43 – 45 ).…”

Section: Discussionmentioning

confidence: 99%

Sustained Drug Treatment Alters the Gut Microbiota in Rheumatoid Arthritis

et al. 2021

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Several studies have investigated the causative role of the microbiome in the development of rheumatoid arthritis (RA), but changes in the gut microbiome in RA patients during drug treatment have been less well studied. Here, we tracked the longitudinal changes in gut bacteria in 22 RA patients who were randomized into two groups and treated with Huayu-Qiangshen-Tongbi formula (HQT) plus methotrexate (MTX) or leflunomide (LEF) plus MTX. There were differences in the gut microbiome between untreated (at baseline) RA patients and healthy controls, with 37 species being more abundant in the RA patients and 21 species (including Clostridium celatum) being less abundant. Regarding the functional analysis, vitamin K2 biosynthesis was associated with RA-enriched bacteria. Additionally, in RA patients, alterations in gut microbial species appeared to be associated with RA-related clinical indicators through changing various gut microbiome functional pathways. The clinical efficacy of the two treatments was further observed to be similar, but the response trends of RA-related clinical indices in the two treatment groups differed. For example, HQT treatment affected the erythrocyte sedimentation rate (ESR), while LEF treatment affected the C-reactive protein (CRP) level. Further, 11 species and 9 metabolic pathways significantly changed over time in the HQT group (including C. celatum, which increased), while only 4 species and 2 metabolic pathways significantly changed over time in the LEF group. In summary, we studied the alterations in the gut microbiome of RA patients being treated with HQT or LEF. The results provide useful information on the role of the gut microbiota in the pathogenesis of RA, and they also provide potentially effective directions for developing new RA treatments.

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Section: Discussionmentioning

confidence: 99%

Sustained Drug Treatment Alters the Gut Microbiota in Rheumatoid Arthritis

et al. 2021

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“…It is reported that CA has antibacterial effect against both Gram-positive and Gram-negative bacteria [ 30 – 33 ]. In consideration of the antibiofilm activity of CA enhanced by its metabolites, further investigation was conducted for the antibacterial spectrum so as to compare the antibacterial effect of CA with its metabolites.…”

Section: Resultsmentioning

confidence: 99%

Quinic acid: a potential antibiofilm agent against clinical resistant Pseudomonas aeruginosa

Lü

Zhao

et al. 2021

Chin Med

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Background The biofilm state of pathogens facilitates antimicrobial resistance which makes difficult-to-treat infections. In this regard, it has been found that the compounds screened from plant extracts represent one category of the most promising antibiofilm agents. However, the antibiofilm activities and the active ingredients of plant extracts remain largely unexplored. In this background, the study is (1) to screen out the plant extracts with antibiofilm ability against Pseudomonas aeruginosa, and (2) to identify the active ingredients in the plant extracts and elucidate the underlying mechanism of the antibiofilm activities. Methods Micro-broth dilution method, in vitro biofilm model, LC–MS/MS analysis and P. aeruginosa-mouse infection model were adopted to assess the antibiofilm activity. GC–MS analysis was performed to detect the active ingredients in plasma. RNA-Seq, GO analysis, KEGG analysis and RT-qPCR were adopted to elucidate the underlying mechanism of antibiofilm activities against P. aeruginosa. Results Lonicerae Japonicae Flos (LJF) among 13 plants could exert significant inhibitory effects on bacterial biofilm formation, mobility and toxin release in vitro, and it could exert antibiofilm effect in vivo too. Moreover, quinic acid, as one metabolite of chlorogenic acid, was found as an active ingredient in LJF against the biofilm of P. aeruginosa. The active ingredient significantly inhibited EPS secretion in biofilm formation and maturity and could achieve synergistic antibiofilm effect with levofloxacin. It reduced the biofilm formation by regulating core targets in quorum sensing system. In GO process, it was found that the core targets were significantly enriched in multiple biological processes involving locomotion, chemotaxis and motility mediated by flagellum/cilium, which was related to KEGG pathways such as bacterial chemotaxis, oxidative phosphorylation, ribosome, biofilm formation, cyanoamino acid metabolism and quorum sensing. Finally, the binding of quinic acid with core targets rhlA, rhlR and rhlB were validated by molecular docking and RT-qPCR. Conclusions In summary, the study verified the in vitro and in vivo antibiofilm effects of LJF against P. aeruginosa and elucidated the active ingredients in LJF and its conceivable pharmacological mechanism, indicating that quinic acid could have the potential of an antibiofilm agent against P. aeruginosa and related infections. Graphic abstract

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“…A recent study [ 16 ] showed that tri-CQA was more active than di-CQA against penicillin sensitive and resistant S. aureus strains, as well as methicillin-resistant S. aureus . While the effect of CQA on the virulence factors and pathogenicity of P. aeruginosa is well understood [ 44 , 45 ], the antibacterial potential of di-CQA against P. aeruginosa strains is controversial and requires further investigation. A number of studies have shown a lack or a weak antibacterial activity of this metabolite against P. aeruginosa [ 46 , 47 ], however, others studies have found approximately MIC values similar to ours of di-CQA against P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule

et al. 2020

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Cichorium intybus L. (Asteraceae) is an important industrial crop, as well as a medicinal plant which produces some bioactive compounds implicated in various biological effects with potential applications in human health. Particularly, roots produce hydroxycinnamic acids like 5-caffeoyquinic acid and 3,5-dicaffeoylquinic acid (di-CQA). The present investigation relates to the use of methyl jasmonate for enhancing phenolic compounds accumulation and production in hairy root cultures of C. intybus. Elicitated hairy root growth rate increased 13.3 times compared with the initial inoculum in a period of 14 days and di-CQA production represented about 12% of DW. The elicitation has also promoted the production of tricaffeoylquinic acid never described in the chicory roots and identified as 3,4,5-tricaffeoyquinic acid by means of nuclear magnetic resonance. Our study confirmed the strong anti-oxidant effect of di-CQA. Our results also confirmed globally a selectivity of action of di-CQA against Gram-positive bacteria, in particular against some strains of Staphylococcus and Streptococcus. However, a non-negligible antibacterial activity of di-CQA against Pseudomonas aeruginosa was also underlined (MIC = 0.156 mg.mL−1 against some P. aeruginosa strains). The influence of di-CQA has been explored to evaluate its impact on the physiology of P. aeruginosa. Di-CQA showed no effect on the biofilm formation and the production of extracellular pyocyanin. However, it demonstrated an effect on virulence through the production of pyoverdine with a dose-dependent manner by more than 7-fold when treated at a concentration of 128 µg·mL−1, thus suggesting a link between di-CQA and iron sequestration. This study shows that elicitated hairy root cultures of chicory can be developed for the production of di-CQA, a secondary metabolite with high antibacterial potential.

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The Antibacterial Activity and Mechanism of Chlorogenic Acid Against Foodborne Pathogen Pseudomonas aeruginosa

Cited by 61 publications

References 30 publications

Sustained Drug Treatment Alters the Gut Microbiota in Rheumatoid Arthritis

Sustained Drug Treatment Alters the Gut Microbiota in Rheumatoid Arthritis

Quinic acid: a potential antibiofilm agent against clinical resistant Pseudomonas aeruginosa

MeJA Elicitation of Chicory Hairy Roots Promotes Efficient Increase of 3,5-diCQA Accumulation, a Potent Antioxidant and Antibacterial Molecule

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