The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains

Priyanto, Jepri Agung; Prastya, Muhammad Eka; Astuti, Rika Indri; Kristiana, Rhesi

doi:10.1007/s12010-023-04382-4

Cited by 10 publications

(8 citation statements)

References 46 publications

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“…Selanjutnya diinkubasi selama 24 jam dengan suhu ±37°C. Adanya aktivitas antibakteri dibuktikan dengan munculnya zona hambat di sekitar koloni bakteri endofit (Priyanto et al 2023).…”

Section: Pendahuluanunclassified

Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.)

Putra,

Retnowati,

Cordova

et al. 2024

JSDH

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Nyatoh plant (Palaquium amboinense B.) is native to Indonesia which is known to have the ability to produce potential secondary metabolites. The aim of this study was to evaluate the antibacterial and antioxidant activities from bioactive compounds produced by endophytic bacteria from nyatoh leaves. Five endophytic bacteria were tested against 4 targeted bacteria including Escherichia coli ATCC 8739, Pseudomonas aeruginosa ATCC 15442, Bacillus subtilis ATCC 19659, and Staphylococcus aureus ATCC 6538. Bacterial isolate which had the strongest antibacterial activity was then fermented and extracted. The corresponding extract was further tested on disc diffusion method, determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values, antioxidant evaluation, and analyzed for its bioactive compound components via GC-MS analysis. The results showed that one isolate, namely D1 can produce secondary metabolite which act as antibacterial and antioxidant potentials. This corresponding extract has the strongest MIC and MBC against B. subtilis with value of 39.5 and >78.1 µg/ml and exhibited antioxidant activity against 2,2-difenil-1-pikrilhidrazil (DPPH) with inhibition value of 85.37%. Based on GC-MS analysis, secondary metabolites derived from this potential extract contained some major active constituents including Tributyl acetylcitrate and bis (2-ethylhexyl) ester which might act as antibacterial and antioxidant agents.

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“…Selanjutnya diinkubasi selama 24 jam dengan suhu ±37°C. Adanya aktivitas antibakteri dibuktikan dengan munculnya zona hambat di sekitar koloni bakteri endofit (Priyanto et al 2023).…”

Section: Pendahuluanunclassified

Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.)

Putra,

Retnowati,

Cordova

et al. 2024

JSDH

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“…Bacteria, as the largest group of plant endophytes, have been isolated from many kinds of medicinal plants and widely studied due to their biocontrol functions [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 ]. By reviewing the recent literature on most of the endophytic bacteria of medicinal plants including Bacillus sp., Pseudomonas sp., Enterobacter sp., Agrobacterium sp., etc., and a large number of endophytic bacteria in the roots, stems, and leaves, we collated some of the relevant data of the isolated endophytic bacteria in Table 1 .…”

Section: Medicinal Plants and Their Cultivable Endophyte Resourcesmentioning

confidence: 99%

“…A new crystalline compound 5-(1-hydroxybutyl)-4-methoxy-3-methyl-2h-pyran-2-one (c-hmmp) in the endophytic fungus Colletotrichum acutatum in the medicinal plant Angelica sinensis shows antibacterial, antimalarial, anticancer, antioxidant, and other activities [ 94 ]. The antibacterial compound 1,4-dihydroxy-2-methyl-anthraquinone was also isolated from the endophytic bacteria of Archidendron pauciflorum [ 32 ]. The structures of the above compounds are shown in Figure 1 .…”

Section: Medicinal Components Produced By Endophytes In Medicinal Plantsmentioning

confidence: 99%

“…The structures of the above compounds are shown in Figure 1. At present, many alkaloids, flavonoids, phenolic acids, terpenoids, coumarin other substances with antioxidant activity have been isolated from endophytes and secondary metabolites of medicinal plants [10,11,32,[95][96][97]. These natural antioxida tive substances often have anti-inflammatory, antioxidant, antibacterial, anti-tumor viral and other functions [10,11,98].…”

Section: Medicinal Components Produced By Endophytes In Medicinal Plantsmentioning

confidence: 99%

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Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts

Wang

Zhang

Hao

et al. 2023

Life

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With the increasing demand for medicinal plants and the increasing shortage of resources, improving the quality and yield of medicinal plants and making more effective use of medicinal plants has become an urgent problem to be solved. During the growth of medicinal plants, various adversities can lead to nutrient loss and yield decline. Using traditional chemical pesticides to control the stress resistance of plants will cause serious pollution to the environment and even endanger human health. Therefore, it is necessary to find suitable pesticide substitutes from natural ingredients. As an important part of the microecology of medicinal plants, endophytes can promote the growth of medicinal plants, improve the stress tolerance of hosts, and promote the accumulation of active components of hosts. Endophytes have a more positive and direct impact on the host and can metabolize rich medicinal ingredients, so researchers pay attention to them. This paper reviews the research in the past five years, aiming to provide ideas for improving the quality of medicinal plants, developing more microbial resources, exploring more medicinal natural products, and providing help for the development of research on medicinal plants and endophytes.

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“…In the recent study, we used 4 Bacillus spp. endophytic strains, which isolated from the leaves and roots of A. pauciflorum [17]. Of note, the target bacterial strains utilized in this research were Gram-negative, including P. aeruginosa strain ATCC 15442 and E. coli strain ATCC 8739 along with Gram-positive strains such as S. aureus strain ATCC 6538 and B. subtilis strain ATCC 19659.…”

Section: Source Of Bacterial Strainsmentioning

confidence: 99%

Endophytic Bacillus spp. isolated from Archidendron pauciflorum: Pharmacological property and their phytochemical constituents

PRIYANTO,

PRASTYA,

ASTUTI

et al. 2023

jrp

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Endophytic Bacillus spp. associated with Archidendron pauciflorum could be the potential source of active compounds attributed to the pharmacological property. The present study aimed to determine the antioxidant, antibacterial, antibiofilm activities, and phytochemical profiles of the crude extracts derived from four endophytic Bacillus spp. isolated from A. pauciflorum. Among all extracts tested, extract from Bacillus pseudomycoides strain DJ8 displayed the strongest antioxidant activity against DPPH and ABTS with IC50 values of 24.87±1.81 and 9.18±0.10 µg/mL, respectively. As evaluated by the dual culture-agar diffusion and disc diffusion methods, three out of four Bacillus spp. extracts also exhibited broad spectra of antibacterial activity against four ATCC strains. Among other extracts, B. pseudomycoides strain DJ8 extract became the most active extract inhibiting Staphylococcus aureus strain ATCC 6538, indicated by the lowest MIC (31.25 µg/mL) and MBC (125 µg/mL), which is considered a highly active antibacterial agent. Three extracts attributed to broad spectra antibacterial activity were also capable to inhibit biofilm formation of four ATCC strains, up to 62.59% in 2 MIC of the extract concentration. Phytochemicals (flavonoid, alkaloid, and terpenoid) were present in four endophytic Bacillus extracts. Among other extracts, an extract derived from B. pseudomycoides strain DJ8 contains the highest flavonoid content (40.10±1.18 mg QE/g extract) and phenol (58.50±4.56 mg GAE/g extract). In conclusion, these four endophytic Bacillus spp. could be the source of pharmaceutically valuable compounds attributed to antioxidant, antibacterial, and antibiofilm properties.

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The Antibacterial and Antibiofilm Activities of the Endophytic Bacteria Associated with Archidendron pauciflorum against Multidrug-Resistant Strains

Cited by 10 publications

References 46 publications

Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.)

Aktivitas Antibakteri dan Antioksidan dari Senyawa Bioaktif Asal Bakteri Endofit Tanaman Nyatoh (Palaquium amboinense B.)

Cultivable Endophyte Resources in Medicinal Plants and Effects on Hosts

Endophytic Bacillus spp. isolated from Archidendron pauciflorum: Pharmacological property and their phytochemical constituents

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