The antibacterial effect of Magnolia mouthwash on the levels of salivary Streptococcus mutans in dental plaque: a randomized, single-blind, placebo-controlled trial

Abstract

Background and Objectives: Dental caries is one of the most common chronic diseases around the world. Inhibitory effects of Magnolia Grandiflora bark extract has been proved on tooth decay both in vitro and by using free sugar chewing gum. This research aimed to examine the effect of Magnolia Grandiflora bark mouth-wash on the prevalence of Streptococcus mutans in dental plaque.
 Materials and Methods: This crossover, placebo-controlled, clinical trial study, was performed on a total of twenty participants (aged 18 to 35 years) in both control and intervention groups and four phases. The prevalence of S. mutans was measured in a certain volume of volunteer’s dental plaque at the beginning of the project (phase 1), after the first prescription (phase 2), following the washout period (phase 3) and finally after the second prescription (phase 4) by culture on bacteriology medium. Plaque index and saliva sampling were carried out in follow-up visits by a dentist. The data were analyzed using T-Test (paired and independent) quantitatively.
 Results: There was a significant difference in S. mutans frequency in dental plaque between when the participants used Magnolia mouthwash and when they washed out or used a placebo (p<0.005). Results also showed a significant difference between Magnolia and Placebo groups in the mean count of saliva bacterial colony counts after oral administration in the first and second time (P<0.001 and P<0.004, respectively).
 Conclusion: The current trial showed that Magnolia Grandiflora %0.3 mouthwash tends to decrease the number of S. mutans in dental plaque significantly. Therefore, its mass production and release to the oral health community are suggested. However, further studies with larger sample sizes and varying treatment are required to substantiate the findings of this study.

Magnolia kobus Extract Inhibits Periodontitis-Inducing Mediators in Porphyromonas gingivalis Lipopolysaccharide-Activated RAW 264.7 Cells

“…Its parts such as bark, flowers, and fruits are used in traditional medicine to treat chronic rhinitis and colds [26]. In previous studies on Magnoliae genus, a Streptococcus mutans-inhibiting effect of Magnolia grandiflora [27], anti-inflammatory activity of magnolol isolated from Magnoliae cortex [28], and inflammatory response-controlling effect of Manoliae cortex and zea mays L complex extract have been reported [29]. Magnolin, an active lignan present in Magnolia, has various effects such as anti-cancer, anti-inflammatory, and antioxidant effects [30][31][32].…”

Neuroprotective Potency of Neolignans in Magnolia officinalis Cortex Against Brain Disorders

“…Magnolia officinalis extract mainly including neolignans was used as an overall wellness nutritional supplement in United States and the recommended dosage was 200–800 mg/day per person for a variety of maladies including anxiety, depression, diabetes, inflammation, headache, muscle pain, weight loss, asthma, stroke, bacterial infection etc. ( Poivre and Duez, 2017 ; Bui et al, 2020 ) In addition, neolignans were often available for daily life and added to mints, gums, mouthwash, insecticide, cosmetic products ( Ye et al, 2015 ; Poivre and Duez, 2017 ; Bui et al, 2020 ; Ghorbani et al, 2021 ). Recently, accumulating studies showed that neolignans manifest neuroprotection on some brain disorders.…”