The antibacterial effect of Plectranthus scutellarioides (L.) R.Br. leaves extract against bacteria associated with peri-implantitis

Bismelah, N. A.; Ahmad, Rohaya; Kassim, Zethy Hanum Mohamed; Ismail, Nor Hadiani; Rasol, Nurulfazlina Edayah

doi:10.1016/j.jtcme.2022.07.002

Cited by 8 publications

(5 citation statements)

References 41 publications

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“…A similar cytotoxic effect was demonstrated on diterpenes isolated from the aerial parts of P. scutellarioides by Creeton et al on human multiple myeloma stem cells and cancer cell line RPMI 8226, with IC 50 = 11.2, 11.0, 4.5, and 9.7 µM values, respectively [37]. In turn, Bismelah et al confirmed that the extract from the aerial parts of P. scutellarioides did not show a cytotoxic effect on normal HGF-1 cells, which is in line with our results [11].…”

Section: Discussionsupporting

confidence: 92%

“…These results are consistent with the literature and confirm the presence of these classes of compounds in the tested extracts of the Plectranthus genus. The differences in the presence of other classes of compounds in the parts analyzed is considered normal; this is due to the fact that a plant may require different compounds in its roots and aerial parts to perform similar biological functions [7,11,[32][33][34][35][36].…”

Section: Discussionmentioning

confidence: 99%

“…In Indonesia, however, P. scutellarioides is used fresh, boiled, or infused to treat conditions such as abscesses, ulcers, and ear and eye infections. Its root is used to cure diarrhea, abdominal pain, diabetes, constipation, fever, and menstrual pain [11,12]. The leaves are used to treat pulmonary tuberculosis by the Toraja ethnic group in Indonesia.…”

Section: Introductionmentioning

confidence: 99%

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Biological Properties of Extracts Obtained from In Vitro Culture of Plectranthus scutellarioides in a Cell Model

Kowalczyk,

Sikora,

Merecz-Sadowska

et al. 2024

IJMS

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Plectranthus scutellarioides (L.) R.Br. is a medicinal plant that has long been used in traditional medicine to treat conditions such as abscesses, ulcers, and ear and eye infections. It is known to have a wide range of biological properties, such as antibacterial, antioxidant, antifungal, anti-inflammatory, anti-diabetic and anti-cancer effects. In this study, we established in vitro cultures from both the aerial parts and roots of Plectranthus scutellarioides. Subsequently, we compared the basic phytochemical profile of the obtained extracts and conducted a biological analysis to assess their potential for inducing apoptosis in breast (MCF-7) and lung (A549) cancer cells. Phytochemical analysis by HPLC-MS revealed the presence of compounds belonging to phenolic acids (ferulic, syringic, vanillic, rosmarinic, chlorogenic, caffeic, coumaric, dihydroxybenzoic acids), flavonoids (eriodyctiol and cirsimaritin), and terpenes such as 6,11,12,14,16-Pentahydroxy-3,17diacetyl-8,11,13-abietatrien-7-one, 6,11,12,14,16-Pentahydroxy-3,17-diacetyl5,8,11,13-abietatetraen-7-one, and 3,6,12-Trihydroxy-2-acetyl-8,12-abietadien7,11,14-trione. The results show that both extracts have a cytotoxic and genotoxic effect against MCF-7 and A549 cancer cells, with a different degree of sensitivity. It was also shown that both extracts can induce apoptosis by altering the expression of apoptotic genes (Bax, Bcl-2, TP53, Fas, and TNFSF10), reducing mitochondrial membrane potential, increasing ROS levels, and increasing DNA damage. In addition, it has been shown that the tested extracts can alter blood coagulation parameters. Our results indicate that extracts from in vitro cultures of Plectranthus scutellarioides aerial parts and roots have promising therapeutic application, but further research is needed to better understand the mechanisms of their action in the in vitro model.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological Properties of Extracts Obtained from In Vitro Culture of Plectranthus scutellarioides in a Cell Model

Kowalczyk,

Sikora,

Merecz-Sadowska

et al. 2024

IJMS

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“…The scanning electron microscopy (SEM) was performed as reported by Bismelah et al ( 26 ) with some modifications. The bacteria suspensions were then centrifuged at 10,000×g for 10 min and the supernatant was removed to obtain the pellet.…”

Section: Methodsmentioning

confidence: 99%

Essential oils of Zingiber officinale: Chemical composition, in vivo alleviation effects on TPA induced ear swelling in mice and in vitro bioactivities

Zhang

Zhang²,

Yu³

et al. 2022

Front. Nutr.

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Zingiber officinale (ZO) is a traditional food condiment. The essential oils of Z. officinale (ZOEOs) are known to have multiple bioactivities. In this study, gas chromatography mass spectrometer (GC-MS) analytical method was used to identify active ingredient present in ZOEOs. A total of 41 compounds were identified in ZOEOs. Major components in ZOEOs were zingiberene (19.71%), (+)-β-cedrene (12.85%), farnesene (12.17%), α-curcumene (10.18%) and β-elemene (3.54%). Experimental results of 12-O-tetradecanoylphorbol-13 acetate (TPA) induced ear swelling validation mice model showed that ZOEOs treatment has better anti-inflammatory effect compared with ibuprofen (positive control) at high concentrations. Histological and immunohistochemical analysis showed that ZOEOs significantly decreased COX-2, IL-6 and NF-κB expression in a dose dependent manner. The mRNA levels of COX-2 and NF-κB were also down regulated by the application of ZOEOs. This indicated that ZOEOs exhibited positive effects in ear skin protection. Antibacterial experimental results showed that EOZOs had anti-bacterial effects on Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. DPPH radical scavenging, A549 cell line and LNCaP cell line inhibition results indicated that ZOEOs exhibited potential antioxidant and anti-tumor properties. The findings of these study provide scientific basis on therapeutic use of ZO in food, cosmetic and pharmaceutical industries.

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“…P. scutellarioides plant parts are widely used as medicines in various forms such as infused, boiled, or fresh to treat diseases like an abscess, blain, ulcers, skin infections, inflammations caused in ears or eyes, and rashes, furthermore, the roots of this plant have been widely employed for the treatment of fever, constipation, pulmonary tuberculosis, and diabetes mellitus. , The extract of P. scutellarioides leaves also shows anti-inflammatory, antimicrobial, and antiproliferative properties. , Various species of this plant have also been employed for the synthesis of nanoparticles for biomedical applications. , However, no work has been reported until now for the synthesis of FL Cu NCs using P.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Green-Emissive Copper Nanoclusters from Plectranthus scutellarioides Extract for the Detection of Terbufos in Carrot and Water Samples

Raval,

Mehta,

Jha

et al. 2023

ACS Food Sci. Technol.

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The development of superior sensors that can detect terbufos insecticide in the fields of agriculture and human health is of utmost importance. Herein, green emissive copper nanoclusters (Cu NCs) were fabricated using the extract of Plectranthus scutellarioides (P. scutellarioides) leaves as biological ligands. The synthesized Plectranthus scutellarioides-Cu NCs (P. scutellarioides-Cu NCs) possess green fluorescence (FL) under UV-light and depict excitation-dependent emission behavior at 450 nm showing maximum emission intensity at 510 nm. The dispersive nature and size of the P. scutellarioides-Cu NCs were calculated to be 2.1 ± 0.6 nm using high-resolution transmission electron microscopy (HR-TEM). The as-synthesized P. scutellarioides-Cu NCs are waterdispersive and show good stability. The introduction of terbufos quenched the FL intensity of P. scutellarioides-Cu NCs via a static quenching mechanism. With terbufos concentrations ranging from 0.1 to 4.0 μM, the ratio of I 0 /I of P. scutellarioides-Cu NCs showed a clear linear relationship with a corresponding low detection limit (LOD) of 0.029 μM. The as-synthesized detection methodology was successfully used to analyze terbufos in vegetable (carrot) and water samples with good recovery and relative standard deviation (RSD) values of less than 2. The real-time study was also performed using a paper-strip-based detection method.

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The antibacterial effect of Plectranthus scutellarioides (L.) R.Br. leaves extract against bacteria associated with peri-implantitis

Cited by 8 publications

References 41 publications

Biological Properties of Extracts Obtained from In Vitro Culture of Plectranthus scutellarioides in a Cell Model

Biological Properties of Extracts Obtained from In Vitro Culture of Plectranthus scutellarioides in a Cell Model

Essential oils of Zingiber officinale: Chemical composition, in vivo alleviation effects on TPA induced ear swelling in mice and in vitro bioactivities

Biosynthesis of Green-Emissive Copper Nanoclusters from Plectranthus scutellarioides Extract for the Detection of Terbufos in Carrot and Water Samples

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