The Antibiofilm Efficacy of Gold Nanoparticles Against Acinetobacter baumannii

Al-Shaabani, Mahmood Jasim Mohammed; Turki, Ahmed; Al-Mathkhury, Harith Jabbar Fahad

doi:10.24996/ijs.2020.61.4.6

Cited by 7 publications

(5 citation statements)

References 17 publications

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“…The high prevalence of integron (class I) in A. baumannii isolates can be attributed to several factors, including inappropriate antibiotic use for A. baumannii infections, which leads to high expression of integrons (class I), and, secondly, the ability of integrons' to obtain new gene cassettes, which leads to antibiotic resistance spreading among clinical isolates. Furthermore, integrons Class 1 are more common than the other classes of integrons, presumably because they are found on genetic elements such as transposons and conjugative plasmids [21]…”

Section: The Results Of Integrons Detectionmentioning

confidence: 99%

The Prevalence of Integron Classes Genes Among A. Baumannii Isolates

Mahmood

2022

eijs

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Acinetobacter baumannii has been recently classified as a major threat to public health because it has resistant to almost all antibiotics and there are many reasons that are responsible for conferring this feature to A. baumannii. One of these reasons is integrons so in this study we show the role of the integrons in providing resistance to some antibiotics. A number of 60 isolates were collected from different clinical sources of patients who were admitted to Baghdad hospitals and all isolates were diagnosed using biochemical tests and confirmed using Chrom-ager culture media, and Vitek 2 compact system. The antibiotic susceptibility test was determined during this study using Kirby-Bauer method and the results of susceptibility demonstrate that these bacteria are responsible for providing resistance to Amikacin, Trimethoprim, Piperacillin, Cefepime, Tetracycline, Ampicillin-sulbactam, Imipenem, and levofloxacin. All isolates show high resistance to trimethoprim and low resistance to tetracycline. The presence of integrons in A. baumannii was detected using conventional polymerase chain reactions. The results showed integron class I was found in all 60 isolates with a percentage (100%) while integron class II was found only in 7 isolates with a percentage (11.6%) and the results of detection showed integron class III are not found in the examined isolates. This study conclude that all A. baumnnii isolates had the strongest resistance to various antibiotics, and the class 1 integron appeared to be the most dominant class among class II and III .

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Section: The Results Of Integrons Detectionmentioning

confidence: 99%

The Prevalence of Integron Classes Genes Among A. Baumannii Isolates

Mahmood

2022

eijs

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“…Local studies showed that the sub-inhibitory concentrations of nanoparticles had an antibiofilm activity against Gram-negative bacteria. (Al-Khafaji, 2017, Al-Shaabani et al, 2020.…”

Section: Antimicrobial Activity Resultsmentioning

confidence: 99%

Food Additive Mediated Biosynthesis of AgNPs with Antimicrobial Activity Against Hypermucoviscous Enterotoxigenic Foodborne Klebsiella pneumoniae

AlKhafaji,

Mohsin,

Alshaikh Faqri

2024

Basrah J. Agric. Sci.

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This study was aimed to adopt an ecofriendly method to synthesize nanoparticles with an effective antibacterial and anti-biofilm activity. A food origin hypermucoviscous Klebsiella pneumoniae was isolated from food samples and identified using biochemical tests and the Vitek system. A string test was depended on to identify hypermucoviscous isolates. Enterotoxicity of foodborne hypermucoviscous K. pneumoniae isolates was detected phenotypically using suckling mouse bioassay. Biofilm forming ability was tested for all the isolated bacteria using the Microtiter plate method and Congo Red Agar. A natural food additive Syzygium aromaticum (clove) aqueous extract was used for the biosynthesis of silver nanoparticles in optimized conditions. The biosynthesized clove-silver nanoparticles (Clove-AgNps) were characterized by several techniques, and their antimicrobial and antibiofilm activity was determined. The results of this study revealed that the isolation of 28 K. pneumoniae isolates were isolated from 200 food samples. String test results showed that 16/28 (57.14%) K. pneumoniae isolates were hypermucoviscous. Eleven of these isolates (68.75%) were enterotoxigenic. Using clove aqueous extract as a biological agent was successful in the biosynthesis of AgNPs with an average diameter of 14.12 nm as measured by AFM. The optimum biosynthesis conditions were: 1mM of AgNO3 concentration, pH 7, at 37 °C, and 24 hours. The minimum inhibitory concentration of the clove-AgNPs was detected as 62.5 µg.mL-1. Sub-inhibitory concentration of 31.25 µg.mL-1 of Clove-AgNPs resulted in: 91% decrease in the formed biofilm. It can be concluded that using Syzygium aromaticum is an effective ecofriendly method for AgNPs biosynthesis with excellent antibacterial and anti-biofilm activity against enterotoxigenic hypermucoviscous K. pneumoniae.

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“…In light of the significant rise in biological and bacterial threats, particularly in sectors related to human consumption such as food, food packaging, and water, scientists are driven to create innovative inorganic nanoparticle substances with antibacterial properties. These substances are intended to be both user-friendly and devoid of any adverse side effects [15][16][17]. A discovery by scientists shows promise in the battle against antibiotic resistance by using a laser to trigger the bactericidal activity of AgNPs.…”

Section: Adhesion Aggregation Maturationmentioning

confidence: 99%

Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne

Tawfeeq,

Abdalameer,

Jassim

et al. 2024

Nano Biomedicine and Engineering

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This study aimed to investigate the efficacy of silver nanoparticles (AgNPs) synthesized by cold plasma in inhibiting biofilm formed by Staphylococcus epidermidis both phenotypically and genotypically. The resultant AgNPs were characterized by using different parameters, color change, Xray diffraction (XRD), zeta potential, feld emission scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM) methods. Moreover, their efficacy in inhibiting S. epidermidis biofilm was studied by the microtiter plate method. The results revealed that cold plasma succeeded in the synthesis of AgNPs with a size range of 10-20 nm. Markedly, the present findings indicated a significant decrease (P < 005) in the intensity of all tested biofilm alongside the down-regulation of accumulation-associated protein (aap) and AtlE in the presence of AgNPs as compared with non-treated cells. In conclusion, AgNPs synthesized by cold plasma acted as antibiofilm agents and could be used for pharmaceutical applications.

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The Antibiofilm Efficacy of Gold Nanoparticles Against Acinetobacter baumannii

Cited by 7 publications

References 17 publications

The Prevalence of Integron Classes Genes Among A. Baumannii Isolates

The Prevalence of Integron Classes Genes Among A. Baumannii Isolates

Food Additive Mediated Biosynthesis of AgNPs with Antimicrobial Activity Against Hypermucoviscous Enterotoxigenic Foodborne Klebsiella pneumoniae

Silver Nanoparticles Synthesized by Cold Plasma as an Antibiofilm Agent against Staphylococcus epidermidis Isolated from Acne

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