The antifungal activity of twenty-four southern African Combretum species (Combretaceae)

Masoko, Peter; Picard, J.; Eloff, Jacobus Nicolaas

doi:10.1016/j.sajb.2006.09.010

Cited by 151 publications

(126 citation statements)

References 20 publications

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“…Different solvents are used for extraction of antimicrobial compounds form plants, and the success is largely dependent on the type of solvent used. [22] In E. axillare, the effect of ethanol extract reported in this study is in line with many others that have documented the good extracting ability of methanol from plants. It was observed in E. axillare that chloroform extract, ethyl acetate and hydro alcoholic extracts showing prominent antimicrobial activity against Bacillus subilis, Staphylococcus aureus, E. coli, Shigella sonni, Pseudomonas aeruginosa, Proteus vulgaris, Aspergillus niger, Candida albicans as compared to aqueous and methanol extracts.…”

Section: Anti-ulcer Activity Of Enicostemma Axillare (Lam)raynal Agasupporting

confidence: 91%

In Vitro Anti-Helicobacter Pylori Activity and Gc-MS Analysis of Enicostemma Axillare (Lam). Raynal

Babu¹,

Sheik²,

K³

et al. 2017

Int. J. Pharm. Sci. Drug Res.

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Traditional healers possess a rich knowledge on the use of medicinal plants for the treatment of various diseases. Enicostemma axillare (Lam). Raynal is one plant, used by healer to treat gastric cancer or ulceration. We assessed the phytochemical components of leaf extract for its antibacterial activity, to add value and provide an evidencebase for their traditional use. The antibacterial potential of the leaf extract was tested against Helicobacter pylori using agar well diffusion method. The minimum inhibitory concentration (MIC) of ethanol extract exhibited the (MIC) against H. pylori ranged from 200µg/ml -250µg/ml followed by chloroform extract. Gas chromatography and mass spectrum (GC-MS) analysis confirms the occurrence of different components in the ethanol and chloroform leaf extract of the studied species.

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Section: Anti-ulcer Activity Of Enicostemma Axillare (Lam)raynal Agasupporting

confidence: 91%

In Vitro Anti-Helicobacter Pylori Activity and Gc-MS Analysis of Enicostemma Axillare (Lam). Raynal

Babu¹,

Sheik²,

K³

et al. 2017

Int. J. Pharm. Sci. Drug Res.

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“…These results confirmed the antifungal activities of some Combretum species reported by Masoko et al, (2007). Acetone leaf extracts of 24 Combretum species also contained many different antifungal compounds based on bioautography (Masoko and Eloff, 2006).…”

Section: Antimicrobial Assaymentioning

confidence: 99%

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Ahmed

McGaw

Elgorashi

et al. 2014

Journal of Ethnopharmacology

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Graphical abstractPhenolic-enriched extracts of four Combretum species namely Combretum bracteosum, Combretum padoides, Combretum vendae and Combretum woodii used in South African traditional medicine to treat various ranges diseases were evaluated against some diarrhoeal aetiologies. All the crude extracts and their fractions of varied polarities had good antimicrobial (non-polar fractions) or good antioxidant (polar fractions) activity. The crude extract of all the plant species were cytotoxic to Vero cell lines but did not lead to COX enzyme inhibition. Phenolic-enriched extractsFour Combretum species traditionally used for treating infectious and gastrointestinal complications in southern Africa In vitro assays: antioxidant, anti-inflammation, antimicrobial, cytotoxicity AbstractEthnopharmacological importance: Infections and gastrointestinal (GIT) disorders such as diarrhoea causes many problems in human health and animal production. Many Combretum species are used in traditional medicine to treat various diseases by rural people in Africa and Asia. Much of the work done to date on some species to validate their ethnopharmacological use was on the non-polar or intermediate polarity components. Many species are yet to be studied against relevant disease parameters using more polar extracts. Aims:The polar components were extracted and fractionated by solvent-solvent fractionation to yield fractions of different polarities. The activity of these fractions on different parameters that could be involved in infectious and gastrointestinal track (GIT) disorders was investigated. The cytotoxic activities of the extracts were also determined to evaluate the potential of these extracts to combat diarrhoea in production animals. Materials and Methods:Phenolic-enriched leaf extracts of Combretum bracteosum (Cob), Combretum padoides (Cop), Combretum vendae (Cov) and Combretum woodii (Cow) were obtained by extracting with a mixture of 70% acetone acidified with 1% HCl and n-hexane. The extract was sequentially treated by solvent-solvent fractionation with dichloromethane, ethyl acetate, and butanol to yield fractions with a large variation in polarity. The phenolic constituents of the extracts and fractions were determined using standard procedures. The antioxidant activities were determined using various standard methods. The minimum inhibitory concentrations (MICs) of the crude extracts and fractions against four bacterial and three fungal strains were assessed with a microplate serial dilution method.Cyclooxygenase (COX) and lipoxygenase (LOX) enzyme inhibitory assays and cytotoxicity studies against Vero cells were also carried out.Result: Some of the fractions had much higher antioxidant activity than the positive controls. The average EC 50 values of the extracts for the DPPH and ABTS antioxidant assays were 0.21-12 µg/ml (Cop), 0.25-16 µg/ml (Cov), 0.33-9.41 µg/ml (Cow) and 4.97-85 µg/ml (Cob) respectively while the mean EC 50 values for the positive controls ascorbic acid and trolox were 1.28-1.51 and 1.02-1.19 ...

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“…Most antimicrobial active components that have been identified are not water soluble and thus organic solvent extracts have been found to be more potent (Parekh et al, 2006). In a study by Masoko and Eloff (2006) where they investigated the antifungal activity of Combretum species, from the extracts used, which included hexane, dichloromethane, acetone and methanol, they discovered that acetone and methanol extracted more chemical compounds from the leaves than the other solvents.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial activity of selected Egyptian ethnomedicinal plants

Abdel¹,

Selim²,

Alfy³

et al. 2013

MJM

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Aims:Medicinal plants have recently received the attention of the antimicrobial activity of plants and their metabolites due to the challenge of growing incidences of drug-resistant pathogens. The aims of this study were to determine the antibacterial activities of plant extracts used as ethnomedicinal in Egypt. Methodology and Results: Investigations were carried out to assess the antibacterial efficiency of 11 plant extracts used as ethnopharmacological among Egyptian native people against infectious diseases. Crude methanol, ethanol, chloroform, hexane, acetone and aqueous extract of plants were tested for antibacterial activity in vitro against ten bacterial isolates using the disc diffusion method test. Discs were impregnated with 2 mg/mL of different solvent extracts. Among all the crude extracts, the methanol extract showed the highest activity than other extracts. P. harmala and S. officinalis exhibited highest antibacterial activity against gram positive and negative bacteria while the remaining plants extracts showed less activity. All the plant extracts showed no significant effect against the Bordetella bronchisepta ATCC 4617 except the extracts of M. fragrans and L. sativum. E. coli is the most sensitive microorganism tested, with the lowest MIC value (0.5 mg/mL) in the presence of the plant extract of P. harmala and S. officinalis. Conclusion, significance and impact of study: Results obtained herein, may suggest that the ethnomedicinal Egyptian plants possess antimicrobial activity and therefore, they can be used in biotechnological fields as natural preservative ingredients in food and/or pharmaceutical industry.

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The antifungal activity of twenty-four southern African Combretum species (Combretaceae)

Cited by 151 publications

References 20 publications

In Vitro Anti-Helicobacter Pylori Activity and Gc-MS Analysis of Enicostemma Axillare (Lam). Raynal

In Vitro Anti-Helicobacter Pylori Activity and Gc-MS Analysis of Enicostemma Axillare (Lam). Raynal

Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities

Antibacterial activity of selected Egyptian ethnomedicinal plants

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