The antimicrobial activity of protein elicitor AMEP412 against Streptomyces scabiei

Liu, Quan; Shen, Yongrui; Yin, Kuide

doi:10.1007/s11274-019-2794-7

Cited by 9 publications

(9 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our previous study [22], the yield of AMEP412 in B. subtilis BU412 could reach 0.3 mg/mL, which was equivalent to the yield of some high efficient recombination expression systems. In addition, AMEP412 was reported as a kind of antimicrobial peptide [22], which was not suitable for recombination expression for its antimicrobial activity against the expression bacteria. Considering the above reasons, the production of AMEP412 was still implemented by its source strain B. subtilis BU412.…”

Section: Discussionmentioning

confidence: 85%

“…On the contrary, Bacillus species had good ability to produce various peptides, which were regarded as an important source of antibiotics, enzymes, and insecticides [20,21]. In our previous study [22], the yield of AMEP412 in B. subtilis BU412 could reach 0.3 mg/mL, which was equivalent to the yield of some high efficient recombination expression systems. In addition, AMEP412 was reported as a kind of antimicrobial peptide [22], which was not suitable for recombination expression for its antimicrobial activity against the expression bacteria.…”

Section: Discussionmentioning

confidence: 89%

“…The tendency had large deviation with the cell growth curve. As AMEP412 was found to have inhibition effect on many microbial [22], including some Bacillus strains (data not shown), we presumed that AMEP412 might play an important role in ecological niche competition. When the nutrition was sufficient, the secretion of AMEP412 was not activated; when the nutrition was in shortage, AMEP412 was secreted to inhibit the nutrition consumption by other microbial cells; when the nutrition was exhausted, the production of AMEP412 was reduced due to limitation of nutrition.…”

Section: Discussionmentioning

confidence: 95%

“…The low temperature could not satisfy the cell growth and protein synthesis. As AMEP412 showed good thermo stability [4,22,30], the denaturation or degradation caused by high temperature might not be the real reason for limiting the yield. It was inferred the secretion system that AMEP412 occupied tended to close at high temperature.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Liu

Shen

Yin

2021

Biotechnology & Biotechnological Equipment

Self Cite

View full text Add to dashboard Cite

In previous study, we identified a novel protein elicitor AMEP412 from Bacillus subtilis BU412, which could trigger plant defense response and induce systemic acquired resistance against diseases. In this paper, the production of the new elicitor was optimised. First, optimal carbon source and nitrogen source were determined as glucose and yeast extract by single factor test. Then, effects of seven different factors on AMEP412 production was studied by Plackett-Burman design. Significant factors (yeast extract, culture temperature and culture time) were selected for further optimisation using central composite design by response surface methodology. The optimum conditions for AMEP412 production were yeast extract 21.72 g/L, culture temperature 30.84 °C and culture time 31.84 h. Under these conditions, the yield of AMEP412 was enhanced from 0.32 to 2.39 mg/mL (7.47-fold), which was quite close to the predicted one. This result significantly raised the yield of AMEP412, which laid solid foundation for its application as a biocontrol agent.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Liu

Shen

Yin

2021

Biotechnology & Biotechnological Equipment

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although multidrug resistance bacteria have been increased because of microbial resistance, antibiotic treatments became more challenging to produce and clinically implement. Recent researches documented antibiotic resistance needs Considering the impact of quorum sensing and biofilm, several strategies including cleaning agent, phages, irradiation, and essentials oil have been developed in food factory and drug discovery (Tanver et al, 2017;Liu et al, 2020). Preventing spoilage of food and bacterial infection by using medicinal plants is safe and non-toxic regarding to health status.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis

Meka¹,

Ayana²,

Biru³

2020

Int. J. Curr. Res. Biosci. Plant Biol.

View full text Add to dashboard Cite

The main objective of this study was to isolate bioactive compounds from Ekebergia capensis for antimicrobial, anti-biofilm and anti-quorum sensing activity. The bark of E. capensis was extracted using hexane, ethyl acetate and methanol for 72 hours on maceration at room temperature. Antibacterial activity was evaluated against Staphylococcus aureus and antifungal activity against Candida albicans at 5 and 10 mg/mL concentrations. Anti-biofilm activity was tested using test tube and swarming motility test methods and anti-quorum sensing was evaluated using flask incubation test for violacein inhibition production methods. The results showed ethyl acetate extract of E. capensis and its two isolated compounds exhibited antimicrobial, anti-biofilm and anti-quorum sensing activity. Inhibition zones of antimicrobial activities of the extract against S. aureus and C. albicans ranged between 4-20 mm. 10 mg/mL extract showed inhibition of biofilm formation against S. aureus, anti-swarming activities against P. aeruginosa and S. aureus. Inhibition of violacein production against S. aureus, P. aeruginosa, and C. albicans were 72.6%, 67.7% and 69.3% respectively. The ethyl acetate extract, which showed antimicrobials, anti-biofilm, anti-quorum sensing activities, after silica gel column chromatography furnished two compounds namely; diisobutylphtalate and Sandropin B. The chemical structures of these compounds were done using spectroscopic methods including IR and NMR. The isolated compounds also showed strong antimicrobial, anti-biofilm, and anti-quorum sensing activity. This could be useful to manage infectious diseases caused by biofilm forming microorganisms.

show abstract

Biocontrol Effect of Bacillus subtilis YPS-32 on Potato Common Scab and Its Complete Genome Sequence Analysis

Zhou

Li²,

Peng

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Potato common scab is caused by Streptomyces, which resides in soil and has become a serious disease in potato planting areas worldwide. In this study, we obtained a Bacillus subtilis YPS-32 strain by natural screening, and atmospheric and roomtemperature plasma (ARTP) mutagenesis and field trial results showed that B. subtilis YPS-32 has a control efficacy of 83.70% against potato common scab. The complete genome of B. subtilis YPS-32 was sequenced, and multiple genes related to the synthesis of antibiotics and plant growth promoters were detected. Based on the genomic information for B. subtilis YPS-32, the sf p geneinactivated (related to the synthesis of secondary metabolites) mutant strain B. subtilis YPS-32Δsfp was constructed. Analysis of crude extract metabolites using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) techniques revealed that strain YPS-32 encodes antagonists, such as surfactin and fengycin, which have antimicrobial effects. This study clarifies the mode of action by which B. subtilis YPS-32 antagonizes Streptomyces scabies and provides a reference for further research on antibacterial genes in the future.

show abstract

The antimicrobial activity of protein elicitor AMEP412 against Streptomyces scabiei

Cited by 9 publications

References 27 publications

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Optimised production of protein elicitor AMEP412 by Bacillus subtilis BU412 through response surface methodology

Antimicrobial, anti-biofilm and anti-quorum sensing compounds from the bark of Ekebergia capensis

Biocontrol Effect of Bacillus subtilis YPS-32 on Potato Common Scab and Its Complete Genome Sequence Analysis

Contact Info

Product

Resources

About