The antioxidant and antiapoptotic effects of crocin pretreatment on global cerebral ischemia reperfusion injury induced by four vessels occlusion in rats

Oruç, Serdar; Gönül, Yücel; Tünay, Kamil; Oruç, Oya Akpınar; Bozkurt, Mehmet Fatih; Karavelioğlu, Ergün; Bağcıoğlu, Erman; Coşkun, Kerem; Çeli̇k, Sefa

doi:10.1016/j.lfs.2016.04.028

Cited by 39 publications

(22 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These physical interactions have been demonstrated to partly account for the observed beneficial effects of crocin. Recent studies have investigated the AO role of crocin against toxicity of various compounds (Oruc et al, ). However, further detailed studies on the AO property of crocin are required to elucidate the biochemical mechanism underlying its protective effects against toxicants.…”

Section: Resultsmentioning

confidence: 99%

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Ansari

Ali

Mahmood

2016

Cell Biology International

View full text Add to dashboard Cite

Sodium nitrite (NaNO ) is a common contaminant of drinking water and food and feed chain. Nitrite induces oxidative damage in humans and animals. In this work, we studied the protective effect of crocin, the active constituent of Crocus sativus (saffron), on NaNO -induced oxidative damage in human erythrocytes. Changes in oxidative stress parameters following NaNO incubation of erythrocytes in presence and absence of crocin were determined. It was found that crocin pre-treatment significantly attenuated NaNO -induced oxidative damage of proteins, lipids, and plasma membrane. Crocin reduced the level of methemoglobin, the primary acute effect of nitrite intoxication. It also improved the antioxidant capacity of cells and NaNO -induced morphological changes in erythrocytes. Crocin is thus a potent protective agent against nitrite-induced cytotoxicity.

show abstract

Section: Resultsmentioning

confidence: 99%

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Ansari

Ali

Mahmood

2016

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Interestingly, crocin is among the major active components of saffron and is a pharmacologically active and water‐soluble carotenoid isolated from saffron crocus ( Crocus sativus L .). There are plenty of studies that have exhibited that crocin possesses therapeutic and pharmaceutical properties, including high antioxidant, anti‐inflammatory, and neuroprotective activities either in vivo or in vitro . Therefore, crocin, by acting as an antioxidant or radical scavenger, could protect cells and tissues against oxidative stress damages .…”

Section: Introductionmentioning

confidence: 99%

Effects of crocin in reducing DNA damage, inflammation, and oxidative stress in multiple sclerosis patients: A double‐blind, randomized, and placebo‐controlled trial

Ghiasian

Khamisabadi

Kheiripour

et al. 2019

J Biochem & Molecular Tox

View full text Add to dashboard Cite

Multiple sclerosis (MS) is an autoimmune disease in which the immune system attacks the nerve cells, resulting in neurological disorders. Oxidative stress, free radicals, and neuritis have important roles in MS pathogenesis. Here, we aim to evaluate the effect of crocin on inflammatory markers, oxidative damage, and deoxyribonucleic acid (DNA) damage in the blood of patients with MS. A total of 40 patients were divided into two groups, drug and placebo‐treated groups, using random assignment. Participants of the intervention and control groups received two crocin capsules or placebo per day for 28 days, respectively. Findings revealed a significant decrease in the level of important pathogenic factors in MS, including lipid peroxidation, DNA damage, tumor necrosis factor‐alpha, and interleukin 17 as well as a significant increase in the total antioxidant capacity in the serum of patients treated with crocin compared with the placebo group. Our results suggest the beneficial and therapeutic effects of crocin in MS.

show abstract

“…It was reported that crocin has protective effects against cerebral ischemic reperfusion injury by suppressing cerebral oxidative stress and increasing the capacity of antioxidant enzymes in the brain (Ochiai et al, 2007; Zheng et al, 2007; Sarshoori et al, 2014; Vakili et al, 2014; Oruc et al, 2016). The obvious limitation is that these studies were merely focused on the cerebral, however, our results indicated that neither crocin nor its metabolite, crocetin, could cross the blood-brain barrier.…”

Section: Introductionmentioning

confidence: 99%

Orally Administered Crocin Protects Against Cerebral Ischemia/Reperfusion Injury Through the Metabolic Transformation of Crocetin by Gut Microbiota

Zhang

Geng

et al. 2019

Front. Pharmacol.

View full text Add to dashboard Cite

Our pilot study suggested that orally administered crocin was hardly absorbed into circulatory system, but it was effective against cerebral ischemic/reperfusion (I/R) injury. The pharmacologically active component and targeting site of crocin remain elusive. In this study, the cerebral-protective effect of crocin was evaluated on a rat transient middle cerebral artery occlusion (MCAO) model. Our data showed that oral administration of crocin had better effectiveness in cerebral protection than an intravenous injection. Neither crocin nor its metabolite crocetin were determined in the brain of cerebral I/R rats, indicating a target site of periphery. Abundant crocetin was detected in plasma after oral administration instead of intravenous injection of crocin. Meanwhile, orally administered crocetin showed similar cerebral protection to that of crocin, but this exciting effect was not clearly observed by intravenous administration of crocetin, indicating the importance of crocetin in gut. Moreover, orally administered crocin showed less cerebral-protective effect in pseudo germ-free (pGF) MCAO rats. In vitro and in vivo experiments confirmed that crocin could be deglycosylated to crocetin in gut content of normal rats, rather than that of pGF rats, indicating that gut microbiota facilitated the transformation of crocin into crocetin, which played a key role in the activation of the pharmacological effect. Metabolomic study revealed that microbial-host co-metabolic molecules were significantly perturbed after oral administration of crocin, indicating a regulation on intestinal ecosystem. It was further suggested that gut microbiota may be the potential target of the cerebral-protective effect of crocin.

show abstract

The antioxidant and antiapoptotic effects of crocin pretreatment on global cerebral ischemia reperfusion injury induced by four vessels occlusion in rats

Cited by 39 publications

References 50 publications

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Crocin protects human erythrocytes from nitrite‐induced methemoglobin formation and oxidative damage

Effects of crocin in reducing DNA damage, inflammation, and oxidative stress in multiple sclerosis patients: A double‐blind, randomized, and placebo‐controlled trial

Orally Administered Crocin Protects Against Cerebral Ischemia/Reperfusion Injury Through the Metabolic Transformation of Crocetin by Gut Microbiota

Contact Info

Product

Resources

About