The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway

Abstract

So far, more than 25,000 brain diseases have been shown to be related to oxidative stress. Excessive free radicals and reactive oxygen species (ROS) can attack cells resulting in dysfunctional proteins, lipids, and nucleic acid, finally leading to imbalance of energy metabolism, cell death, gene mutation, and immune reaction. Therefore oxidative stress plays an important role in neuronal diseases. As a traditional Chinese medicine, Zhengtian Pill (ZTP) was reported to have the ability to reduce the blood viscosity of migraine model rats, with increased beta-endorphin, serotonin, adrenaline, and dopamine in brain tissue. Moreover ZTP can effectively accelerate blood circulation and attenuate blood coagulation. However, the molecular mechanisms of ZPT are still unclear. Through the behavioral test we found that ZTP can significantly improve depression-like behavior induced by LPS when rat was treated with ZTP (L 0.17 g/kg, M 0.34 g/kg, and H 0.7 g/kg) intraperitoneal injection once a day for 30 consecutive days. And ZTP can resist oxidative stress (>72 h) for a longer time. And ZTP can promote the levels of ATP and SOD and reduce the levels of ROS and MDA in the brain. At the same time, ZTP can have antioxidant stress through increasing the expression level of Nrf2/HO-1/P38. These results show that ZTP may be a potential antioxidant stress drug for variety of diseases associated with oxidative stress injury.

The Anti-Depression-Like Effects of Zhengtian Capsule via Induction of Neurogenesis and the Neurotrophic Signaling Pathway

“…In our study, we applied single administration of LPS for establishing a short-term effect of depression-like behavior. This study, combined with our previous work, found that the administration of ZTC is basically safe and will not cause any adverse effects on the normal life of animals ( Yang et al., 2019a ). We continuously monitored weight gains and food intake in both experiments, and neither showed significant differences.…”

“…These traditional Chinese herbs are supplied by the China Resources Sanjiu Medical & Pharmaceutical Co., Ltd. The complexity of ZTC has been analyzed by 2D-LC system that 876 peaks were detected and the peak capacity reached 1740 (please refer to the detailed information in literatures ( Wei et al., 2009 ; Yang et al., 2019a ). Resveratrol (RES, Sigma-Aldrich, USA).…”

“…Our recent studies have demonstrated that ZTC can alleviate the oxidative stress of Kunming mice. It is observed that, under the induction of lipopolysaccharide (LPS), administration of ZTC could down-regulate the levels of H 2 O 2 and Malondialdehyde, and upregulate the levels of SOD and ATP in brain tissue, thus reducing the depression-like behavior caused by oxidative stress ( Yang et al., 2019a ).…”

Zhibaidihuang Decoction Ameliorates Cell Oxidative Stress by Regulating the Keap1‐Nrf2‐ARE Signalling Pathway

“…e Keap1-Nrf2-ARE signalling pathway regulates the expression of a series of antioxidant enzymes including SOD, which is an important antioxidant enzyme in organisms and the primary scavenger of free radicals [43,44]. is action may be an important mechanism by which ZBDHD ameliorates the symptoms of Yin deficiency and fire exuberance in various diseases.…”