The antiviral effects of acteoside and the underlying IFN-γ-inducing action

Abstract

There are many herbal teas that are found in nature that may be effective at treating the symptoms and also shortening the duration of viral infections. When combating viral infections, T lymphocytes are an indispensable part of human acquired immunity. However, studies on the use of natural products in stimulating lymphocyte-mediated interferon-gamma (IFN-γ) production are very limited. In this study, we found that acteoside, a natural phenylpropanoid glycoside from Kuding Tea, enhanced IFN-γ production in mouse lymphocytes in a dose-dependent manner, particularly in the CD4+ and CD8+ subsets of T lymphocytes. To this end, we suggest that the antiviral activity of acteoside was highly correlated to its inducing ability of IFN-γ production. Mechanistically, the activation of T-bet enhanced the promoter of IFN-γ and subsequently resulted in an increased IFN-γ production in T cells. Collectively, we have found a natural product with the capacity to selectively enhance mouse T cell IFN-γ production. Given the role of IFN-γ in the immune system, further studies to clarify the role of acteoside in inducing IFN-γ and prevention of viral infection are needed.

Anti-Respiratory Syncytial Virus Activity of Plantago asiatica and Clerodendrum trichotomum Extracts In Vitro and In Vivo

“…Specifically, intraperitoneal treatment with acteoside showed an anti-RSV effect in a mouse model (Figure 6C). These results are similar to the antiviral activity of acteoside against Influenza or VSV [28]. However, further studies demonstrating a detailed mechanism of how acteoside inhibit RSV replication are needed.…”

“…To further evaluate the therapeutic effect of acteoside against RSV, we investigated its antiviral activity in BALB/c mice. Mice were intranasally infected with RSV-GFP (1 × 10 6 PFU/mouse), and acteoside was administrated intraperitoneally at 6 hpi at a dose of 80 mg/kg, as described previously [28]. Lungs from all mice were collected at 3 dpi and 5 dpi, and RSV-G mRNA was measured by qRT-PCR.…”

“…Reportedly, acteoside has anti-oxidant and anti-inflammatory properties, and prevents cell apoptosis [39,49]. A recent study demonstrated that acteoside induced ERK activation and subsequent IFN-γ production; thus, showed antiviral activity against influenza and vesicular stomatitis virus (VSV) [28]. Based on these reported findings, we evaluated the antiviral effect of acteoside against RSV.…”

Therapeutic potential of phenylethanoid glycosides: A systematic review

“…And the antiviral mechanism of verbascoside was related to the activation of ERK and enhancement of IFN-γ production. 148 Forsythiaside and calceolarioside B showed significant antiviral potentialon respiratory syncytial virus in vitro. 149 Forsythiaside inhibited the infectivity of avian infectious bronchitis virus.…”

Effect and mechanism of verbascoside on hypoxic memory injury in plateau

“…et Mey, Phlomis younghusbandii Mukerjee and so on. Number of studies have proved verbascoside with remarkable pharmacological activities, including antioxidant (Cardinali et al, ), neuroprotective (Sheng, Zhang, Pu, Ma, & Li, ), enhanced memory (Zhou et al, ), anti‐aging (Nam, Kim, & Jeong, ), antibacterial and anti‐inflammatory (Motojima, Villareal, Iijima, Han, & Isoda, ; Oyourou, Combrinck, Regnier, & Marston, ; Speranza et al, ), antivirus (Song et al, ), and antitumor (Alipieva, Korkina, Orhan, & Georgiev, ; Esposito et al, ), etc.…”