The anxiolytic-like effects of Aloysia polystachya (Griseb.) Moldenke (Verbenaceae) in mice

Hellión-Ibarrola, M.C.; Ibarrola, D.A.; Montalbetti, Y.; Kennedy, María L.; Heinichen, O.; Campuzano, M.; Tortoriello, Jaime; Fernandez, Sebastian P.; Wasowski, Cristina; Marder, Mariel; Lima, Thereza Christina Monteiro de; Mora, Sergio

doi:10.1016/j.jep.2005.11.013

Cited by 58 publications

(35 citation statements)

References 34 publications

(27 reference statements)

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“…Drugs that increase open arm exploration are considered as anxiolytics. [10] In our study, we observed that PGFJ (100, 200 and 400 mg/kg) and Diazepam (2 mg/kg) induced significant increase in both the number of entries and time spent in the open arms. The number of entries and time spent in the closed arms were reduced in the PGFJ treated groups as compared to the control group.…”

Section: Discussionsupporting

confidence: 46%

Evaluation of Punica granatum fruit juice for anti-anxiety activity

Kulkarni¹,

Bathe²,

Javalgikar³

2016

Int J of Biomed & Adv Res

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Background and Objectives: In traditional system of Indian medicine, Punica granatum L. fruits are widely used for treatment of brain diseases, fever, heart diseases, diarrhea, dysentery, piles, inflammation and bronchitis. However, there is no authentic scientific data reported regarding anxiolytic activity of Punica granatum (Linn) fruit juice. To evaluate the anxiolytic activities of Punica granatum L. fruit juice in various validated animal models of anxiety in mice. Methods: The preliminary phytochemical investigation was carried out with the fruit juice of Punica granatum for qualitative identification of phytoconstituents. Animals were administered with orally 0.1, 0.2, 0.4 and 0.8 ml of Punica granatum fruit juice and observed for its mortality upto 48 hours study period (short term toxicity) to determine LD 50 . From the LD 50 dose, 1/20, 1/10 & 1/5 doses were selected and considered as low, medium and high dose respectively. For assessing the anxiolytic activity, models like Elevated Plus Maze, Hole-Board were used Diazepam was used as a standard reference for anxiolytic. Results: Preliminary phytochemical investigation of the PGFJ (Punica granatum L. fruit juice) revealed the presence of flavonoids, saponins, tannins, sterols, polyphenols, alkaloids, carbohydrates and proteins. LD 50 (acute oral toxicity) of Punica granatum fruit juice was determined in mice with a dose limit of 2000 mg/kg as per OECD guidelines no.425 and even upto 2000 mg/kg dose no mortality was recorded. Hence the experimental doses selected were 1/20, 1/10 and 1/5 of the LD 50 value and considered as low, medium and high doses of Punica granatum fruit juice 100, 200 and 400 mg/kg respectively. In Elevated Plus Maze model, low, medium and high doses PGFJ and Diazepam (2 mg/kg) had significantly increased number of entries, time spent in open arms and decreased the number of entries and time spent in closed arms. In Hole-board model, Diazepam (2 mg/kg), medium and high doses (200 and 400mg/kg) but not the low dose (100mg/kg) of PGFJ had significantly increased the number of head dips, latency of first head dip and number of rearings. Conclusion: The present study ascertains that the plant Punica granatum L. fruit juice possesses significant antianxiety activity in mice and study concludes the beneficial effects of Punica granatum fruit juice in treatment of anxiety signifies the rational basis for its traditional use.

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Section: Discussionsupporting

confidence: 46%

Evaluation of Punica granatum fruit juice for anti-anxiety activity

Kulkarni¹,

Bathe²,

Javalgikar³

2016

Int J of Biomed & Adv Res

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“…One hour after drug administration, each mouse was placed in the arena, and its ambulation in the peripheral area, rearing, grooming, and defecation were recorded for 5 min (Hellión-Ibarrola et al, 2006). The number of gridlines crossed by both hind feet in a 5 min period was counted as an index of ambulation.…”

Section: Open-field Testmentioning

confidence: 99%

Anxiolytic-like effects of acute and chronic treatment with Achillea millefolium L. extract

Baretta

Felizardo

Bimbato

et al. 2012

Journal of Ethnopharmacology

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“…Each mouse was placed individually in a cylindrical glass chamber saturated for 20 min with vapor delivered from 5 ml of diethyl-ether absorbed on a piece of cotton bound in the coiled cap of the flask. Each mouse was left there for 60 s after loss of its postural reflex -sleep latency was defined as time until loss of the postural reflex -and then was withdrawn and placed in an individual cage to record the time to recover the postural reflex (sleep duration) (Héllión-Ibarrola et al, 2006).…”

Section: Sleep Induced By Diethyl-ether (Sid)mentioning

confidence: 99%