The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated spermatozoa

Gomez‐Lopez, Nardhy; Estrada-Gutiérrez, Guadalupe; Colin, Alinne; Flores-Pliego, Arturo; Flores-Escobar, Xochitl; Oehninger, Sergio; Barroso, Gerardo

doi:10.1016/j.fertnstert.2012.11.052

Cited by 8 publications

(7 citation statements)

References 39 publications

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“…However, and in agreement with our results, when a lower quantity of apoptotic bodies was detected, there was an absence of statistical correlation between apoptotic bodies and semen quality (Gomez‐Lopez et al. ) in human samples. Another explanation for the absence of correlation between apoptotic bodies and semen quality in fresh samples may be due to the stallions used in this study, which did not present any pathology, disease or surgery that could affect the reproductive capability, in contrast to all of the previously published studies (Marchiani et al.…”

Section: Discussionsupporting

confidence: 92%

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Identification of Apoptotic Bodies in Equine Semen

Caselles

Miró-Morán

Rodríguez

et al. 2014

Reprod Domestic Animals

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Apoptosis in the testis is required to ensure an efficient spermatogenesis. However, sometimes, defective germ cells that are marked for elimination during this process escape elimination in the testes, giving rise to ejaculates with increased percentages of abnormal and apoptotic spermatozoa and a high percentage of apoptotic bodies. Apoptosis markers in the ejaculate have been associated with low fertility, either in animals or humans. Therefore, the goal of this study was to investigate whether fresh equine semen contains apoptotic bodies [initially named Merocyanine 540 (M540) bodies] and to study the relationship between the quantity of these bodies and cell concentration, the volume of ejaculate, viability and motility. Moreover, we also studied whether the presence apoptotic bodies in fresh semen was related to the resistance of the stallion spermatozoa to being incubated at 37 °C or being frozen and thawed. Fresh equine semen was stained with fluorescent dyes such as M540 and Annexin-V. Active Caspase 3 was studied in fresh semen through Western blotting and immunofluorescence with a specific antibody. Sperm kinematics was assessed in fresh, incubated and thawed samples using computer-assisted semen analysis, and viability was evaluated with the LIVE/DEAD Sperm Viability Kit. Overall, our results demonstrate for the first time the presence of apoptotic bodies in equine semen. The quantity of apoptotic bodies was highly variable among stallions and was positively correlated with Caspase 3 activity in fresh samples and negatively correlated with the viability and motility of stallion spermatozoa after the cryopreservation process.

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Section: Discussionsupporting

confidence: 92%

“…; Gomez‐Lopez et al. ). The quantity of apoptotic bodies found in equine semen was lower than those reported previously (Lotti et al.…”

Section: Discussionmentioning

confidence: 98%

Identification of Apoptotic Bodies in Equine Semen

Caselles

Miró-Morán

Rodríguez

et al. 2014

Reprod Domestic Animals

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“…51 Our present study suggested that the higher percentage of DNA fragmentation observed in infertile smokers along with negative significant, semen parameters, and age of study subjects. Gomez et al 52 have strongly proved that the smoking affects sperm DNA, leads to fragmentation; also in fertilization process, couples those who have gone for assisted reproductive technology (ART), intra cytoplasmic injection (ICSI) and in vitro fertilization (IVF) got a negative impact on apoptotic markers of caspase and annexin-V. Same way El-Karaksy results also strongly stated tobacco smoking is one of the major reasons for insufficient antioxidant scavenging activity, mainly done by increasing the production of ROS which is attributed to sperm DNA fragmentation in infertile smokers.…”

Section: Cigarette Smoking and Caspase-3mentioning

confidence: 99%

Deterioration of semen quality and sperm‐DNA integrity as influenced by cigarette smoking in fertile and infertile human male smokers—A prospective study

Ranganathan

Rao

Balasundaram

2019

J of Cellular Biochemistry

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In modernized lifestyle smoking is one of the trendy, psychological, and socioeconomic scenarios of young adolescents mainly in the age of the reproductive stage. Based on a number of cigarettes smoked, age, and duration of the smoke, the study aims to search for the profound effects of smoking and its impact on semen parameters, sperm-DNA integrity, and fragmentation of sperm DNA with cotinine and apoptotic caspase-3 marker in the seminal plasma of fertile and infertile smokers. To determine oxidative damage by 8-hydroxy deoxyguanosine (8-OHdG) from isolated sperm DNA (steps: reactive oxygen species washing by nitro blue tetrazolium (NBT), sperm lysis, salt digestion, ethanol washing, and finally with high-performance liquid chromatography analysis). Level of DNA fragmentation (percentage) in native and intact DNA, the activity of caspase-3 in infertile smokers will be compared with the control group of nonsmokers. Also, the sperm viability was visualized by eosin-nigrosin and aniline blue staining. Cotinine is one of the best markers of smoking. The cotinine level (2224.24 ± 1.19 *** ng/mL), when abundant it negative correlates with morphology and rapid motility in infertile smokers than nonsmokers. Gel preprogram measured the sperm integrity and was found to be less in smokers than nonsmokers. The spermatic oxidative marker 8-OHdG was high and gave an R 2 value of 0.9104 with morphology and 0.9007 for rapid motility of infertile sperm, respectively. Infertile smoking subjects (<10 cigarettes/day) had significant changes increase in sperm fragmentation, caspase-3, and cotinine while negative impact with motility, morphology, and pH of semen compared with fertile, infertile nonsmoking subjects. K E Y W O R D S 8-hydroxy deoxyguanosine, caspase-3, nicotine, sperm-DNA fragmentation

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“…The labeling of DNA strand breaks (the terminal transferased UTP nick-end labeling (TUNEL) assay), Annexin V-propidium iodide assay and Sperm Chromatin Structure Assay are used for the detection and quantification of apoptosis in sperm (DNA fragmentation) (Erenpreisa et al 2002, Ricci et al 2002, Oosterhuis and Vermes 2004, Mahfouz 2009, Rylander et al 2009, Gatimel et al 2013, Gomez-Lopez et al 2013, Ruvolo et al 2013.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Lasienë

Gedrimas

Vitkus

et al. 2013

Polish Journal of Veterinary Sciences

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The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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The apoptotic pathway in fertile and subfertile men: a case-control and prospective study to examine the impact of merocyanine 540 bodies on ejaculated spermatozoa

Cited by 8 publications

References 39 publications

Identification of Apoptotic Bodies in Equine Semen

Identification of Apoptotic Bodies in Equine Semen

Deterioration of semen quality and sperm‐DNA integrity as influenced by cigarette smoking in fertile and infertile human male smokers—A prospective study

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

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