The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance

Chen, Pengxiang; Yan, Minmin; Li, Lei; He, Jieqiang; Zhou, Shuangxi; Li, Zhongxing; Niu, Chundong; Bao, Chana; Zhi, Fang; Ma, Fengwang; Guan, Qingmei

doi:10.1038/s41438-020-00419-5

Cited by 45 publications

(25 citation statements)

References 76 publications

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“…Using the TNT SP6 High‐Yield Wheat Germ Protein Expression System (Promega, NO.L3260, USA), these cDNAs were transcribed and translated in vitro . DAP‐qPCR and ChIP‐qPCR were performed as described previously (Chen et al., 2020b; Xie et al., 2018). The primers are listed in Table S1.…”

Section: Methodsmentioning

confidence: 99%

MdGH3.6 is targeted by MdMYB94 and plays a negative role in apple water‐deficit stress tolerance

et al. 2021

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Drought significantly limits apple fruit production and quality. Decoding the key genes involved in drought stress tolerance is important for breeding varieties with improved drought resistance. Here, we identified GRETCHEN HAGEN3.6 (GH3.6), an indole-3-acetic acid (IAA) conjugating enzyme, to be a negative regulator of water-deficit stress tolerance in apple. Overexpressing MdGH3.6 reduced IAA content, adventitious root number, root length and water-deficit stress tolerance, whereas knocking down MdGH3.6 and its close paralogs increased IAA content, adventitious root number, root length and water-deficit stress tolerance. Moreover, MdGH3.6 negatively regulated the expression of wax biosynthetic genes under water-deficit stress and thus negatively regulated cuticular wax content. Additionally, MdGH3.6 negatively regulated reactive oxygen species scavengers, including antioxidant enzymes and metabolites involved in the phenylpropanoid and flavonoid pathway in response to water-deficit stress. Further study revealed that the homolog of transcription factor AtMYB94, rather than AtMYB96, could bind to the MdGH3.6 promoter and negatively regulated its expression under water-deficit stress conditions in apple. Overall, our results identify a candidate gene for the improvement of drought resistance in fruit trees.

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Section: Methodsmentioning

confidence: 99%

MdGH3.6 is targeted by MdMYB94 and plays a negative role in apple water‐deficit stress tolerance

et al. 2021

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“…The coding region of MdNB‐ARC was cloned into the vector of pRI101 and the resulting plasmid was transformed into Agrobacterium tumefaciens GV3101. The stably transgenic apple plants were obtained as described previously (Chen et al ., 2020). To analyse the anthocyanin accumulation of MdNB‐ARC transgenic plants, the shoot segments of tissue‐cultured plants were transferred to Murashige and Skoog (MS) media containing 3% (normal concentration) and 6% (high concentration) sucrose with a long day photoperiod (light:dark, 14 h:10 h) for 30 days.…”

Section: Methodsmentioning

confidence: 99%

Insights into the effect of human civilization on Malus evolution and domestication

Chen

Zhang

et al. 2021

Plant Biotechnology Journal

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The evolutionary history of the Malus genus has not been well studied. In the current study, we presented genetic evidence on the origin of the Malus genus based on genome sequencing of 297 Malus accessions, revealing the genetic relationship between wild species and cultivated apples. Our results demonstrated that North American and East Asian wild species are closer to the outgroup (pear) than Central Asian species, and hybrid species including natural (separated before the Pleistocene, about 2.5 Mya) and artificial hybrids (including ornamental trees and rootstocks) are between East and Central Asian wild species. Introgressions from M. sylvestris in cultivated apples appeared to be more extensive than those from M. sieversii, whose genetic background flowed westward across Eurasia and eastward to wild species including M. prunifolia, M. × asiatica, M. × micromalus, and M. × robust. Our results suggested that the loss of ancestral gene flow from M. sieversii in cultivated apples accompanied the movement of European traders around the world since the Age of Discovery. Natural SNP variations showed that cultivated apples had higher nucleotide diversity than wild species and more unique SNPs than other apple groups. An apple ERECTA‐like gene that underwent selection during domestication on 15th chromosome was identified as a likely major determinant of fruit length and diameter, and an NB‐ARC domain‐containing gene was found to strongly affect anthocyanin accumulation using a genome‐wide association approach. Our results provide new insights into the origin and domestication of apples and will be useful in new breeding programmes and efforts to increase fruit crop productivity.

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“…Leaves were collected from control and drought-treated M. prunifolia seedlings. Total RNA was extracted using the cetyltrimethylammonium bromide (CTAB) method according to a previously described procedure [ 40 ]. The RNA-seq library was constructed as previously reported by Xie et al [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Profiling of N6-Methyladenosine (m6A) Modification Landscape in Response to Drought Stress in Apple (Malus prunifolia (Willd.) Borkh)

Mao

Hou

Liu

et al. 2021

Plants

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Drought stress is a significant environmental factor limiting crop growth worldwide. Malus prunifolia is an important apple species endemic to China and is used for apple cultivars and rootstocks with great drought tolerance. N6-methyladenosine (m6A) is a common epigenetic modification on messenger RNAs (mRNAs) in eukaryotes which is critical for various biological processes. However, there are no reports on m6A methylation in apple response to drought stress. Here, we assessed the m6A landscape of M. prunifolia seedlings in response to drought and analyzed the association between m6A modification and transcript expression. In total, we found 19,783 and 19,609 significant m6A peaks in the control and drought treatment groups, respectively, and discovered a UGUAH (H: A/U/C) motif. In M. prunifolia, under both control and drought conditions, peaks were highly enriched in the 3′ untranslated region (UTR) and coding sequence (CDS). Among 4204 significant differential m6A peaks in drought-treated M. prunifolia compared to control-treated M. prunifolia, 4158 genes with m6A modification were identified. Interestingly, a large number of hypermethylated peaks (4069) were stimulated by drought treatment compared to hypomethylation. Among the hypermethylated peak-related genes, 972 and 1238 differentially expressed genes (DEGs) were up- and down-regulated in response to drought, respectively. Gene ontology (GO) analyses of differential m6A-modified genes revealed that GO slims related to RNA processing, epigenetic regulation, and stress tolerance were significantly enriched. The m6A modification landscape depicted in this study sheds light on the epigenetic regulation of M. prunifolia in response to drought stress and indicates new directions for the breeding of drought-tolerant apple trees.

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The apple DNA-binding one zinc-finger protein MdDof54 promotes drought resistance

Cited by 45 publications

References 76 publications

MdGH3.6 is targeted by MdMYB94 and plays a negative role in apple water‐deficit stress tolerance

MdGH3.6 is targeted by MdMYB94 and plays a negative role in apple water‐deficit stress tolerance

Insights into the effect of human civilization on Malus evolution and domestication

Profiling of N6-Methyladenosine (m6A) Modification Landscape in Response to Drought Stress in Apple (Malus prunifolia (Willd.) Borkh)

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