The Application of Antibiotics and Sulphonamide for Eliminating Bacillus Cereus During the Micropropagation of Infected Dieffenbachia Picta Schott

Zenkteler, E.; Wlodarczak, K; Kłosowska, M.

doi:10.1007/978-94-015-8951-2_22

Cited by 3 publications

(3 citation statements)

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“…In recent years, it became evident that serovars of S. enterica are not only able to attach to and proliferate on the surface of plant tissues (Zenkteler et al, 1997;Solomon et al, 2002) but can also colonize plant tissues internally (Kutter et al, 2006). For example, gfp-tagged strains of S. enterica colonized the interior of tomato plants when grown hydroponically (Guo et al, 2001;Guo et al, 2002) and various S. enterica serovars were able to colonize Medicago sativa and other leguminous plants endophytically and epiphytically (Dong et al, 2003;Wang et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency

et al. 2007

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The availability of knowledge of the route of infection and critical plant and microbe factors influencing the colonization efficiency of plants by human pathogenic bacteria is essential for the design of preventive strategies to maintain safe food. This research describes the differential interaction of human pathogenic Salmonella enterica with commercially available lettuce cultivars. The prevalence and degree of endophytic colonization of axenically grown lettuce by the S. enterica serovars revealed a significant serovar-cultivar interaction for the degree of colonization (S. enterica CFUs per g leaf), but not for the prevalence. The evaluated S. enterica serovars were each able to colonize soil-grown lettuce epiphytically, but only S. enterica serovar Dublin was able to colonize the plants also endophytically. The number of S. enterica CFU per g of lettuce was negatively correlated to the species richness of the surface sterilized lettuce cultivars. A negative trend was observed for cultivars Cancan and Nelly, but not for cultivar Tamburo. Chemotaxis experiments revealed that S. enterica serovars actively move toward root exudates of lettuce cultivar Tamburo. Subsequent micro-array analysis identified genes of S. enterica serovar Typhimurium that were activated by the root exudates of cultivar Tamburo. A sugar-like carbon source was correlated with chemotaxis, while also pathogenicity-related genes were induced in presence of the root exudates. The latter revealed that S. enterica is conditioned for host cell attachment during chemotaxis by these root exudates. Finally, a tentative route of infection is described that includes plant-microbe factors, herewith enabling further design of preventive strategies.

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Section: Introductionmentioning

confidence: 99%

Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency

et al. 2007

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“…(19,44) are especially of major concern due to the environmental occurrence of these bacteria. The presence of human-pathogenic bacteria has been described for a wide range of plant hosts (7,17,18,19,21,34,37,39,44,47). For greenhouse-grown vegetables, these pathogens are suggested to be introduced as a result of bad hygiene during the production or postharvest processing of the crops (1,7).…”

mentioning

confidence: 99%

“…Thus, contamination of plants with humanpathogenic bacteria from manure may occur, for example, during rainfall or irrigation due to splashing of soil and bacteria onto the plants (34). Alternatively, plants could be colonized via the roots in manure-amended soil (39,47). The colonization of plants via the roots by human-pathogenic E. coli was observed using a gfp-tagged strain of E. coli O157:H7 that colonized the interior of lettuce from soil via the roots up to the leaves (16,39).…”

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confidence: 99%

Physiological and Molecular Responses of Lactuca sativa to Colonization by Salmonella enterica Serovar Dublin

Klerks

Gent-Pelzer

Franz

et al. 2007

Appl Environ Microbiol

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This paper describes the physiological and molecular interactions between the human-pathogenic organism Salmonella enterica serovar Dublin and the commercially available mini Roman lettuce cv. Tamburo. The association of S. enterica serovar Dublin with lettuce plants was first determined, which indicated the presence of significant populations outside and inside the plants. The latter was evidenced from significant residual concentrations after highly efficient surface disinfection (99.81%) and fluorescence microscopy of S. enterica serovar Dublin in cross sections of lettuce at the root-shoot transition region. The plant biomass was reduced significantly compared to that of noncolonized plants upon colonization with S. enterica serovar Dublin. In addition to the physiological response, transcriptome analysis by cDNA amplified fragment length polymorphism analysis also provided clear differential gene expression profiles between noncolonized and colonized lettuce plants. From these, generally and differentially expressed genes were selected and identified by sequence analysis, followed by reverse transcription-PCR displaying the specific gene expression profiles in time. Functional grouping of the expressed genes indicated a correlation between colonization of the plants and an increase in expressed pathogenicity-related genes. This study indicates that lettuce plants respond to the presence of S. enterica serovar Dublin at physiological and molecular levels, as shown by the reduction in growth and the concurrent expression of pathogenicity-related genes. In addition, it was confirmed that Salmonella spp. can colonize the interior of lettuce plants, thus potentially imposing a human health risk when processed and consumed.

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Movement and containment of microbial contamination in the nutrient mist bioreactor

Sharafeldin

Weathers

2006

In Vitro Cell. Dev. Biol. - Plant

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The Application of Antibiotics and Sulphonamide for Eliminating Bacillus Cereus During the Micropropagation of Infected Dieffenbachia Picta Schott

Cited by 3 publications

References 5 publications

Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency

Differential interaction of Salmonella enterica serovars with lettuce cultivars and plant-microbe factors influencing the colonization efficiency

Physiological and Molecular Responses of Lactuca sativa to Colonization by Salmonella enterica Serovar Dublin

Movement and containment of microbial contamination in the nutrient mist bioreactor

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