The application of fluorescent in situ hybridization to detect Mbcr/abl fusion in variant Ph chromosomes in CML and ALL

Dewald, Gordon W.; Schad, Chris R.; Christensen, Eric; Tiede, Amy L.; Zinsmeister, Alan R.; Spurbeck, Jack L.; Thibodeau, Stephen N.; Jalal, Syed M.

doi:10.1016/0165-4608(93)90196-s

Cited by 115 publications

(46 citation statements)

References 10 publications

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“…Other cells randomly analyzed (erythroid precursors, neutrophils, and rare monocytes, and lymphocytes/plasma cells showed two red and two green signals (Figure 2), except for rare cells with juxtaposed signals (three cells of 183 total cells studied in patient 1, and one of 180 cells in patient 2), which is within the range of false positive cells (Ͻ2-3%) usually expected for this probe set. 15,16 …”

Section: Resultsmentioning

confidence: 99%

Pseudo-Gaucher histiocytes identified up to 1 year after transplantation for CML are BCR/ABL-positive

et al. 1998

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The results of polymerase chain reaction (PCR) analysis after transplantation for chronic myelogenous leukemia (CML) are difficult to interpret clinically. Positive findings for BCR/ABL can be seen not only in patients who go on to relapse but also in patients who, after years of follow-up, remain in complete remission. The cause for the lack of concordance between PCR findings and relapse is not clear. We identified two patients with CML who had rare pseudo-Gaucher cells in their bone marrow aspirate specimens prior to, and at 1, and 6 or 12 months following syngeneic or allogeneic hematopoietic transplantation. After the transplant, the patients obtained clinical remission and were shown to be cytogenetically normal and to have germline MBCR in blood or bone marrow by Southern analysis. One patient was PCR-positive for BCR/ABL in the marrow at 12 months. In order to determine whether the pseudo-Gaucher histiocytes were BCR/ABL-positive, we used fluorescence in situ hybridization and probes for MBCR and ABL and analyzed Wright-stained smears to correlate molecular cytogenetic findings with cell type. On three aspirate smears from each patient (at 6 or 12 months post-transplant), all of the pseudo-Gaucher cells studied (10/10 in one patient and 12/12 in the other) showed the fusion for BCR/ABL. Other cells analyzed randomly (erythroid precursors, granulocytes and rare monocytes, lymphocytes and plasma cells) did not. Our cases provide the first proof that pseudo-Gaucher cells carry the BCR/ABL fusion. Furthermore, they illustrate that these cells can be found in the marrow for up to 12 months following transplantation. Our results permit speculation that pseudo-Gaucher cells or other long-lived histocytes may be one cause of persistent PCR positivity after transplantation that is not predictive of disease relapse.

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Section: Resultsmentioning

confidence: 99%

Pseudo-Gaucher histiocytes identified up to 1 year after transplantation for CML are BCR/ABL-positive

et al. 1998

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“…A similar BCR/ABL1 fusion occurs in 17% of adults with ALL and 1% of patients with AML [14]. Some investigators have suggested that these Ph-positive forms of acute leukemia may be similar to lymphoid or myeloid blast crisis of CML [15].…”

Section: Discussionmentioning

confidence: 99%

Applications of Fluorescence in Situ Hybridization (FISH) for Detecting Genetic Changes in Hematological Malignancies

Taştemir¹,

Demirhan²,

Gürkan³

et al. 2011

JCT

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“…A possible disadvantage for FISH is high false-positivity. Dewald et al 13 reported earlier that the normal range for the interphase bone marrow cells with apparent fusion signals was р10%. In the present study after considerable improvement of the technique, however, false positivity was less than 4% in both peripheral and bone marrow cells.…”

Section: Discussionmentioning

confidence: 99%

“…Although several investigators have reported that the twocolor interphase FISH could identify Ph-positive cells in peripheral blood, as well as in bone marrow in patients with CML, [10][11][12][13][14][15][16][17][18][19][20] there has been no study in a sufficient number of CML patients except one published recently. 21 Here, presenting the data of simultaneously performed RT-PCR, we also report that interphase FISH analysis in peripheral blood is a very simple and reliably sensitive technique for the diagnosis of CML, as well as for the quantitative monitoring of leukemia cell population during therapy.…”

Section: Introductionmentioning

confidence: 99%

Simple and reliably sensitive diagnosis and monitoring of Philadelphia chromosome-positive cells in chronic myeloid leukemia by interphase fluorescence in situ hybridization of peripheral blood cells

et al. 1999

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The application of fluorescent in situ hybridization to detect Mbcr/abl fusion in variant Ph chromosomes in CML and ALL

Cited by 115 publications

References 10 publications

Pseudo-Gaucher histiocytes identified up to 1 year after transplantation for CML are BCR/ABL-positive

Pseudo-Gaucher histiocytes identified up to 1 year after transplantation for CML are BCR/ABL-positive

Applications of Fluorescence in Situ Hybridization (FISH) for Detecting Genetic Changes in Hematological Malignancies

Simple and reliably sensitive diagnosis and monitoring of Philadelphia chromosome-positive cells in chronic myeloid leukemia by interphase fluorescence in situ hybridization of peripheral blood cells

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