The Application of Nanomagnetic Approaches for Targeting Adipose Derived Stem Cells for Use in Tendon Repair

Thomas, Diana; Markides, Hareklea; Tony, YU; Chen, Guoqiang; Liu, Wei; Alicia, J El Haj

doi:10.1166/jnn.2016.12739

Cited by 2 publications

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“…Magnetic nanoparticle (MNP)-based technologies are new multimodal tools designed to achieve therapeutic functions in cell-based orthopaedic therapies, such as tracking, targeting and activation [ 5 , 6 , 7 , 8 , 9 ]. One such novel technology is the use of MNPs to deliver functional mechanical cues directly to cells in vivo, which has been shown to regulate cell differentiation and downstream signalling [ 1 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Short-Term Evaluation of Cellular Fate in an Ovine Bone Formation Model

Markides

Foster

McLaren

et al. 2021

Cells

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The ovine critical-sized defect model provides a robust preclinical model for testing tissue-engineered constructs for use in the treatment of non-union bone fractures and severe trauma. A critical question in cell-based therapies is understanding the optimal therapeutic cell dose. Key to defining the dose and ensuring successful outcomes is understanding the fate of implanted cells, e.g., viability, bio-distribution and exogenous infiltration post-implantation. This study evaluates such parameters in an ovine critical-sized defect model 2 and 7 days post-implantation. The fate of cell dose and behaviour post-implantation when combined with nanomedicine approaches for multi-model tracking and remote control using external magnetic fields is also addressed. Autologous STRO-4 selected mesenchymal stromal cells (MSCs) were labelled with a fluorescent lipophilic dye (CM-Dil), functionalised magnetic nanoparticles (MNPs) and delivered to the site within a naturally derived bone extracellular matrix (ECM) gel. Encapsulated cells were implanted within a critical-sized defect in an ovine medial femoral condyle and exposed to dynamic gradients of external magnetic fields for 1 h per day. Sheep were sacrificed at 2 and 7 days post-initial surgery where ECM was harvested. STRO-4-positive (STRO-4+) stromal cells expressed osteocalcin and survived within the harvested gels at day 2 and day 7 with a 50% loss at day 2 and a further 45% loss at 7 days. CD45-positive leucocytes were also observed in addition to endogenous stromal cells. No elevation in serum C-reactive protein (CRP) or non-haem iron levels was observed following implantation in groups containing MNPs with or without magnetic field gradients. The current study demonstrates how numbers of therapeutic cells reduce substantially after implantation in the repair site. Cell death is accompanied by enhanced leucocyte invasion, but not by inflammatory blood marker levels. Crucially, a proportion of implanted STRO-4+ stromal cells expressed osteocalcin, which is indicative of osteogenic differentiation. Furthermore, MNP labelling did not alter cell number or result in a further deleterious impact on stromal cells following implantation.

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Section: Introductionmentioning

confidence: 99%

Short-Term Evaluation of Cellular Fate in an Ovine Bone Formation Model

Markides

Foster

McLaren

et al. 2021

Cells

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Effects of Iron Nanoparticles on Immune Response of Two Immunocytes Like Virus

Zhang¹,

Chen²,

Lin³

et al. 2017

nanosci nanotechnol lett

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Virus usually induces strong immune response, which plays the most critical role in immunotherapy. As virus-like particles, nanoparticles may have the similar immune effects. By performing in-depth datum mining of global gene expression profiles, this study investigated the effects of DMSA-coated Fe3O4 magnetic nanoparticles (named FeNPs) on the immune system of four mammalian cells at various doses for different times. The results revealed that the FeNPs triggered comprehensive immune responses of two immunocytes (RAW264.7 and THP-1) like virus, inducing production of many kinds of cytokines. Especially, nearly half of differentially expressed genes (DEGs) were found being interferon-stimulated genes (ISGs), which are closely related to antivirus. These virus-like effects showed dose-dependent feature and immunocyte specificity. This study thus firstly reported the systematic effects on the immune response at the gene transcription level, and also suggests that FeNPs may be developed into useful immuno nanoinducers.

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The Application of Nanomagnetic Approaches for Targeting Adipose Derived Stem Cells for Use in Tendon Repair

Cited by 2 publications

References 0 publications

Short-Term Evaluation of Cellular Fate in an Ovine Bone Formation Model

Short-Term Evaluation of Cellular Fate in an Ovine Bone Formation Model

Effects of Iron Nanoparticles on Immune Response of Two Immunocytes Like Virus

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