The application of polythiol molecules for protein immobilisation on sensor surfaces

Development of Non-Labeled QCM Biosensor for the Detection of <i>β</i>-Galactosidase: A Comparative Study of Gold and Polystyrene Nanoparticles

“…Nanofilms on transducer surfaces have the advantage of allowing easy miniaturization and creation of novel functional interfaces. Selfassembled monolayer of compounds containing thiol groups have shown to be an attractive mean to chemically modify surfaces for various applications like controlling surface wettability, structuring surfaces, binding of species such as metal ions, nanoparticles, and biomolecules [8][9][10][11].…”

Towards the design of universal immunosurfaces for SPR-based assays: A review

“…Last trends in SAM based-immobilization models employ oligo-or poly-ethylene glycols (OEG or PEG) as spacers to enhance the assay sensitivity [16]. The functionalization of gold substrates based on polythiols, PEG and thiol-peptides [17] exploits the linkage between amine and thioacetal groups to form stable monolayers. In particular, PEG SAMs prevent nonspecific adsorption of proteins due to their antifouling capacity to overcome undesirable steric effects [18].…”

Sensitive immunosensor for benzo[a]pyrene detection based on dual amplification strategy of PAMAM dendrimer and amino-modified methylene blue/SiO2 core–shell nanoparticles

“…Physical adsorption is simple and convenient; however, the weakness of non-covalent bond may lead to gradual elution of adsorbed proteins. Self-assembly is convenient and relatively stable, but the reaction is limited (Kyprianou et al, 2010). Other approaches such as attaching by functional polymers (chitosan and nafion) or enzyme-entrapping membranes exhibit excellent stability, but the worse control in layer formation brings uncertainty and poor reproducibility of the sensor.…”