The application of proteomics in the diagnosis and treatment of  bronchial asthma

Xu, Peng; Wang, Lingwei; Chen, Dandan; Feng, Mingyang; Lu, Yongzhen; Chen, Rongchang; Qiu, Chen; Li, Jie

doi:10.21037/atm.2020.02.30

Cited by 22 publications

(14 citation statements)

References 30 publications

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“…In the long-standing task of finding specific, easily-detectable and reliable biomarkers of pulmonary diseases, much work has been dedicated to alternative biofluids such as bronchoalveolar lavage fluid [18]. Many studies on the BALF proteome have being made all over the world, especially targeting widespread diseases such as lung cancer [19,20], cystic fibrosis [21,22], asthma [23,24] and chronic obstructive pulmonary disease [25,26]. Nonetheless, BALF proteomics applied to the study of interstitial lung diseases has gaining momentum and is providing reliable new results.…”

Section: Discussionmentioning

confidence: 99%

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Shaba¹,

Landi²,

Carleo³

et al. 2021

Preprint

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In the longtime challenge of identifying specific, easily-detectable and reliable biomarkers of Idiopathic Pulmonary Fibrosis (IPF), bronchoalveolar lavage fluid (BALF) proteomics is providing interesting new insights into its pathogenesis. To the best of our knowledge, the present study is the first shotgun proteomic investigation of EVs isolated from BALF of IPF patients. Our main aim was to characterize the proteome of the vesicular component of BALF and to explore its individual impact on the pathogenesis of IPF. To this purpose, ultracentrifugation was chosen as EVs isolation technique and their purification was assessed by TEM, 2DE and LC-MS/MS. Our 2DE data and scatter plots showed considerable differences between the proteome of EVs and that of whole BALF and of its fluid component. Analysis of protein content and protein functions evidenced that EV proteins are predominantly involved in cytoskeleton remodeling, adenosine signaling, adrenergic signaling, C-peptide signaling and lipid metabolism. Our findings may suggest a wider system involvement in the disease pathogenesis and support the importance of pre-fractioning of complex samples, like BALF, in order to let low-abundant proteins-mediated pathways to emerge.

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Section: Discussionmentioning

confidence: 99%

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Shaba¹,

Landi²,

Carleo³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In the long-standing task of finding specific, easily detectable and reliable biomarkers of pulmonary diseases, much work has been dedicated to alternative biofluids such as BALF [18]. Many studies on the BALF proteome have being made targeting widespread diseases such as lung cancer [19,20], cystic fibrosis [21,22], asthma [23,24] and chronic obstructive pulmonary disease [25,26]. Nonetheless, BALF proteomics applied to the study of ILDs could provide reliable new results.…”

Section: Discussionmentioning

confidence: 99%

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Shaba

Landi

Carleo

et al. 2021

IJMS

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In the longtime challenge of identifying specific, easily detectable and reliable biomarkers of IPF, BALF proteomics is providing interesting new insights into its pathogenesis. To the best of our knowledge, the present study is the first shotgun proteomic investigation of EVs isolated from BALF of IPF patients. Our main aim was to characterize the proteome of the vesicular component of BALF and to explore its individual impact on the pathogenesis of IPF. To this purpose, ultracentrifugation was chosen as the EVs isolation technique, and their purification was assessed by TEM, 2DE and LC-MS/MS. Our 2DE data and scatter plots showed considerable differences between the proteome of EVs and that of whole BALF and of its fluid component. Analysis of protein content and protein functions evidenced that EV proteins are predominantly involved in cytoskeleton remodeling, adenosine signaling, adrenergic signaling, C-peptide signaling and lipid metabolism. Our findings may suggest a wider system involvement in the disease pathogenesis and support the importance of pre-fractioning of complex samples, such as BALF, in order to let low-abundant proteins-mediated pathways emerge.

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“…The two‐tailed t test method was used to calculate the p ‐value. When p ‐value <0.05, the change of the differential expression amount exceeding 2 was used as the significant upregulation change threshold 15 …”

Section: Methodsmentioning

confidence: 99%

A novel prognosis prediction model, including cytotoxic T lymphocyte‐associated antigen‐4, ischemia‐modified albumin, lipoprotein‐associated phospholipase A2, glial fibrillary acidic protein, and homocysteine, for ischemic stroke in the Chinese hypertensive population

Shen

Bao

et al. 2021

Clinical Laboratory Analysis

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Background There is still a lack of tools to assess the prognosis of ischemic stroke patients induced by hypertension. In this study, we built a novel prognostic assessment model for ischemic stroke in the Chinese hypertensive population. Methods Mass spectrometry technique was used to analyze the changes in serum protein profiles of hypertensive patients with ischemic stroke. A total of 314 hypertensive patients were divided into the testing group (206 patients) and the validation group (108 patients). Results Compared with hypertensive patients without ischemic stroke, serum cytotoxic T lymphocyte‐associated antigen‐4 (CTLA‐4), ischemia‐modified albumin (IMA), lipoprotein‐associated phospholipase A2 (Lp‐PLA2), glial fibrillary acidic protein (GFAP), and homocysteine (HCY) levels were significantly increased among hypertensive patients with ischemic stroke (p < 0.05). Then, we built a novel prognostic assessment model for hypertensive patients with ischemic stroke [Logit(P) = 29.172–1.088*CTLA‐4–0.952*IMA‐0.537*Lp‐PLA2 −0.066*GFAP −0.149*HCY]. It showed higher efficiency (AUC = 0.981, sensitivity = 95.5%, specificity = 93.8%) than any single marker. The estimated probability was 0.739, which means if higher than 0.739, it was classified into poor prognosis. Compared with the estimated probability ≤0.739 group, the survival rate of hypertensive patients with ischemic stroke in the estimated probability >0.739 group was significantly decreased (χ2 = 40.001, p < 0.001). In the validation group, our novel prognostic assessment model still showed good efficiency (AUC = 0.969, sensitivity = 89.4%, specificity = 92.5%; χ2 = 47.551, p < 0.001). Conclusion Current novel prognostic assessment model we have built is of great value in the prognostic evaluation for ischemic stroke in the Chinese hypertensive population.

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The application of proteomics in the diagnosis and treatment of bronchial asthma

Cited by 22 publications

References 30 publications

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

Proteome Characterization of BALF Extracellular Vesicles in Idiopathic Pulmonary Fibrosis: Unveiling Undercover Molecular Pathways

A novel prognosis prediction model, including cytotoxic T lymphocyte‐associated antigen‐4, ischemia‐modified albumin, lipoprotein‐associated phospholipase A2, glial fibrillary acidic protein, and homocysteine, for ischemic stroke in the Chinese hypertensive population

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