The Arabidopsis Receptor Kinase IRK Is Polarized and Represses Specific Cell Divisions in Roots

Campos, Roya; Goff, Jason; Rodríguez-Furlán, Cecilia; Norman, Jaimie M. Van

doi:10.1016/j.devcel.2019.12.001

Cited by 35 publications

(61 citation statements)

References 82 publications

(99 reference statements)

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“…Within the root meristem, cells proliferate in longitudinal files extending shootward through transverse anticlinal divisions. In the root ground tissue, longitudinal anticlinal cell divisions rarely occur in the meristem with these divisions producing more ground tissue cells around the stele (Campos et al, 2020). Following proliferation in the meristem, cells undergo dramatic elongation which accounts for the majority of root lengthening (Hodge et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…In the Arabidopsis root the LRR-RLK, IRK, is polarly localized to the outer (lateral) polar domain in endodermal cells; yet, in other root cell types, it is localized to distinct domains or is nonpolar (Campos et al, 2020). irk mutants have excess longitudinal anticlinal and periclinal endodermal cell divisions and show increased stele area in the radial axis.…”

Section: Introductionmentioning

confidence: 99%

“…PXC2 was recently also given the name CANALIZATION-RELATED RECEPTOR-LIKE KINASE (CANAR) and shown to coordinate PIN-FORMED 1 (PIN1) localization during auxin canalization with canar mutants having defects in cotyledon and leaf venation and vascular regeneration after wounding (Hajný et al, 2020). As PXC2 and IRK are both expressed in the root tip (Wang et al, 2013;Campos et al, 2020), we hypothesized that these LRR-RLKs have similar and/or overlapping functions in root development.…”

Section: Introductionmentioning

confidence: 99%

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PXC2, a polarized receptor kinase, functions to repress ground tissue cell divisions and restrict stele size

Goff¹,

Jm²

2021

Preprint

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In plants, coordination of cell division and differentiation is critical for tissue patterning and organ development. Directional cell signaling and cell polarity have been proposed to participate in coordination of these developmental processes. For instance, a leucine-rich repeat receptor-like kinase (LRR-RLK) named INFLORESCENCE AND ROOT APICES KINASE (IRK) functions to restrict stele area and inhibit longitudinal anticlinal divisions (LADs) in the endodermis where it is polarly localized. The LRR-RLK most closely related to IRK is PXY/TDR CORRELATED 2 (PXC2) and we find that PXC2 shows similar polarized accumulation as IRK in root cell types. To further understand how these proteins operate in directional cell-cell signaling and root development we explored PXC2 function. pxc2 roots have an increase in stele area, indicating that PXC2 also functions to restrict stele size. Additionally, compared to either single mutant, irk pxc2 roots have an enhanced phenotype with further increases in endodermal LADs and stele area indicating redundant activities of these receptors. The double mutant also exhibits abnormal root growth, suggesting broader functions of PXC2 and IRK in the root. However, PXC2 is not functionally equivalent to IRK, as endodermal misexpression of PXC2 did not fully rescue irk. We propose that PXC2 is at least partially redundant to IRK with a more predominant role for IRK in repression of endodermal LADs. Our results are consistent with the hypothesis that repression of specific endodermal cell divisions and stele area through a PXC2/IRK-mediated directional signaling pathway is required for coordinated root growth and development.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PXC2, a polarized receptor kinase, functions to repress ground tissue cell divisions and restrict stele size

Goff¹,

Jm²

2021

Preprint

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“…Membrane-localized polarity complexes often orient metazoan asymmetric divisions [20]. While mutations in, or overexpression of, polarity proteins can affect division patterns in Arabidopsis [21,22], whether the intracellular positions of these polarized complexes directly orient divisions remains unknown. To test whether the BASL/ BRXf crescent is the spatial landmark required for NM pre and NM post , we first monitored nuclear movement in basl cotyledons (basl-2 35S::PIP2A-RFP ML1::H2B-YFP) ( Figure 2A).…”

Section: Nuclear Migration Is Dominated By Cell-autonomous Polarity Smentioning

confidence: 99%

Opposing, Polarity-Driven Nuclear Migrations Underpin Asymmetric Divisions to Pattern Arabidopsis Stomata

2020

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“…Whether MUS and MUL interact with PIN1 in vivo needs to be investigated in the future. It is worth noting that another member in the LRR VII subfamily, INFLORESCENCE AND ROOT APICES RECEPTOR KINASE (IRK), also shows polar distribution in the endodermis of Arabidopsis primary roots (Campos et al , 2020). Whether additional members in this subfamily show polar localization is another interesting question to be explored.…”

Section: Discussionmentioning

confidence: 99%

Two receptor‐like protein kinases, MUSTACHES and MUSTACHES‐LIKE, regulate lateral root development in Arabidopsis thaliana

Xun

et al. 2020

New Phytologist

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Receptor-like protein kinases (RLKs) play key roles in regulating plant growth, development and stress adaptations. There are at least 610 RLKs (including receptor-like cytoplasmic kinases) in Arabidopsis. The functions of the majority of RLKs have not yet been determined. We previously generated promoter::GUS transgenic plants for all leucine-rich repeat (LRR)-RLKs in Arabidopsis and analyzed their expression patterns during various developmental stages. We found the expression of two LRR-RLKs, MUSTACHES (MUS) and MUSTACHES-LIKE (MUL), are overlapped in lateral root primordia. Independent mutants, mus-3 mul-1 and mus-4 mul-2, show a significantly decreased emerged lateral root phenotype. Our analyses indicate that the defects of the double mutant occur mainly at stage I of lateral root development. Exogenous application of auxin can dramatically enhance the transcription of MUS, which is largely dependent on AUXIN RESPONSE FACTOR 7 (ARF7) and ARF19. MUS and MUL are inactive kinases in vitro but are phosphorylated in planta, possibly by an unknown kinase. The kinase activity of MUS is dispensable for its function in lateral root development. Many cell wall related genes are down regulated in mus-3 mul-1. In conclusion, we identified MUS and MUL, two kinase-inactive RLKs, in controlling the early development of lateral root primordia likely via regulating cell wall synthesis and remodeling.

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The Arabidopsis Receptor Kinase IRK Is Polarized and Represses Specific Cell Divisions in Roots

Cited by 35 publications

References 82 publications

PXC2, a polarized receptor kinase, functions to repress ground tissue cell divisions and restrict stele size

PXC2, a polarized receptor kinase, functions to repress ground tissue cell divisions and restrict stele size

Opposing, Polarity-Driven Nuclear Migrations Underpin Asymmetric Divisions to Pattern Arabidopsis Stomata

Two receptor‐like protein kinases, MUSTACHES and MUSTACHES‐LIKE, regulate lateral root development in Arabidopsis thaliana

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