The Arabidopsis TTL3 protein interconnects brassinosteroid signalling and cytoskeleton during lateral root development

Abstract

Lateral roots are essential components of the plant edaphic interface, contributing to water and nutrient uptake, biotic and abiotic interactions, stress survival, and plant anchorage. We have identified the TETRATRICOPEPTIDE-REPEAT THIOREDOXIN-LIKE 3 (TTL3) being related to lateral root emergence and later development. TTL3 interacts with microtubules and potentially interconnects cytoskeletal function with the brassinosteroid signalling pathway. Loss of function of TTL3 leads to a reduced number of emerged lateral roots due to delayed development of lateral root primordia. Lateral root growth of the ttl3 mutant is less sensitive to BR treatment. Timing and spatial distribution of TTL3 expression is consistent with its role in development of lateral root primordia before their emergence and subsequent development into lateral roots. TTL3 is a novel component of the root system morphogenesis regulatory network.

Direct non-transcriptional link between brassinosteroid perception and cortical microtubule reorientation drives hypocotyl growth

“…MBAPs could also serve as scaffolds as it has been shown for BSK3 and TETRATRICOPEPTIDE THIOREDOXIN-LIKE (Amorim-Silva et al, 2019;Ren et al, 2019). Especially the fact that TTL3 localizes to CMTs suggests possible functions of CMTs in this process (Xin et al, 2020).…”

“…Since the BR transcriptional reprograming is not affected in mbap2-mbap3, the BR responses driven by MBAPs likely involve their interaction with BRI1 and non-genomic responses through CMT reorganization. BRI1 dynamics was previously reported to require CMTs (Bücherl et al, 2017;Ruan et al, 2018;Xin et al, 2020). To better understand the connection between BRI1 and CMTs, we first carried out immunoprecipitation of the functional BRI1-mCit fusion, stably expressed in transgenic plants under the control of BRI1 promoter, coupled to mass spectrometry analyses.…”

Transcriptome analysis reveals key regulatory genes for root growth related to potassium utilization efficiency in rapeseed (Brassica napus L.)

“…A strong association was found between B naA05G0032700ZS and four genes, BnaC06G0226000ZS, BnaC02G0500800ZS , BnaC01G0183000ZS, and BnaA09G0485400ZS in the green-yellow module ( Figure S4A ). A homolog of BnaA05G0032700ZS identified as tetratricopeptide-repeat thioredoxin-like 3 (TTL3) interfaces with microtubules and may be a link between cytoskeletal function and the brassinosteroid signaling pathway in the formation of lateral roots ( Xin et al., 2020 ).…”