The ARC2 response in Streptomcyes coelicolor requires the global regulatory genes afsR and afsS

Calvelo, Vanessa Yoon; Crisante, David; Elliot, Marie A.; Nodwell, Justin R.

doi:10.1099/mic.0.001047

Cited by 4 publications

(3 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, some SARPs, such as NosP in S. actuosus, can also respond to secondary metabolites and their intermediates (Li et al, 2018). Recently, the global regulator AfsR in S. coelicolor was shown to play an important role in the effect of the artificial elicitor ARC2 (Calvelo et al, 2021). This suggested that some signaling compounds were probably needed for the activation effect of AfsR.…”

Section: Discussionmentioning

confidence: 99%

The roles of SARP family regulators involved in secondary metabolism in Streptomyces

Yan,

Xia

2024

Front. Microbiol.

View full text Add to dashboard Cite

Streptomyces species are best known for their ability to produce abundant secondary metabolites with versatile bioactivities and industrial importance. These metabolites are usually biosynthesized through metabolic pathways encoded by cluster-situated genes. These genes are also known as biosynthetic gene clusters (BGCs) of secondary metabolites. The expression of BGCs is intricately controlled by pyramidal transcriptional regulatory cascades, which include various regulators. Streptomyces antibiotic regulatory proteins (SARPs), a genus-specific family of regulators, are widely distributed and play important roles in regulating the biosynthesis of secondary metabolites in Streptomyces. Over the past decade, the biological functions of SARPs have been extensively investigated. Here, we summarized the recent advances in characterizing the roles of SARPs involved in Streptomyces secondary metabolism from the following three aspects. First, the classification and domain organization of SARPs were summarized according to their size variation. Second, we presented a detailed description of the regulatory mechanisms and modes of action of SARPs involved in secondary metabolism. Finally, the biotechnological application of SARPs was illustrated by improving the production of target secondary metabolites and discovering novel bioactive natural products. This review will help researchers to comprehensively understand the roles of SARPs in secondary metabolite biosynthesis in Streptomyces, which will contribute to building a solid foundation for their future application in synthetic biology.

show abstract

Section: Discussionmentioning

confidence: 99%

The roles of SARP family regulators involved in secondary metabolism in Streptomyces

Yan,

Xia

2024

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…This helps to explain the difference in NP induction observed between ARC2 and ARC6. More recently, Nodwell and co-workers delved deeper into the mechanism of ARC2 activation (Calvelo et al, 2021 ). To explore this, RNA-seq analysis of S. coelicolor treated with ARC2 was performed.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, it is likely that ARC2 induction of NPs is through activation of AfsS via a global stress response (Fig. 4A–B ) (Calvelo et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Small molecule inducers of actinobacteria natural product biosynthesis

Alwali,

Parkinson

2023

Journal of Industrial Microbiology and Biotechnology

View full text Add to dashboard Cite

Actinobacteria are a large and diverse group of bacteria that are known to produce a wide range of secondary metabolites, many of which have important biological activities, including antibiotics, anti-cancer agents, and immunosuppressants. The biosynthesis of these compounds is often highly regulated with many natural products being produced at very low levels in laboratory settings. Environmental factors, such as small molecule elicitors, can induce the production of secondary metabolites. Specifically, they can increase titers of known natural products as well as enabling discovery of novel natural products typically produced at undetectable levels. These elicitors can be natural products, including antibiotics or hormones, or synthetic compounds. In recent years, there has been a growing interest in the use of small molecule elicitors to induce the production of secondary metabolites from actinobacteria, especially for the discovery of natural products from “silent” biosynthetic gene clusters. This review aims to highlight classes of molecules that induce secondary metabolite production in actinobacteria and to describe the potential mechanisms of induction.

show abstract

Crosstalk between primary and secondary metabolism: Interconnected fatty acid and polyketide biosynthesis in prokaryotes

West

Bailey

2023

Bioorganic & Medicinal Chemistry Letters

View full text Add to dashboard Cite

The ARC2 response in Streptomcyes coelicolor requires the global regulatory genes afsR and afsS

Cited by 4 publications

References 62 publications

The roles of SARP family regulators involved in secondary metabolism in Streptomyces

The roles of SARP family regulators involved in secondary metabolism in Streptomyces

Small molecule inducers of actinobacteria natural product biosynthesis

Crosstalk between primary and secondary metabolism: Interconnected fatty acid and polyketide biosynthesis in prokaryotes

Contact Info

Product

Resources

About