The art of signal transforming: electrodes and their smart applications in electrochemical sensing

Li, Zhanming; Yu, Yue; Li, Zhiliang; Wu, Tao; Yin, Jinjin

doi:10.1039/c5ay02373d

Cited by 14 publications

(9 citation statements)

References 175 publications

(152 reference statements)

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“…In short, the signal change for SPEs were less than that of the hybrid device. Our results indicate similar characteristics with the literature [50].…”

Section: Electrochemical Performance Of Chip Under Ambient and Elevat...supporting

confidence: 92%

“…This indicates the surface roughness was considerably higher for the SPEs. The difference is a consequence of the rough surface of the SPE, leading to less controllable formation of the self-assembled monolayer (SAM) with more variable surface coverage than that of the smooth chip which results in the improved performance of the chip in the hybridisation experiments with non-complementary target at 50 °C [49][50][51][52].…”

Section: Surface Characterization Of Spes and The Chipmentioning

confidence: 99%

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An electrochemical biosensor with integrated microheater to improve the sensitivity of electrochemical nucleic acid biosensors

Akcakoca¹,

Ghorbanpoor²,

Blair³

et al. 2022

J. Micromech. Microeng.

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Electrochemical impedance spectroscopy (EIS) is often used for biomolecular detection based on the interaction of a molecule with a receptor functionalised electrode surface and consequent impedance change. Though its performance is well established, there is still a need for improved sensitivity and specificity, especially when attempting to detect nucleic acids from clinical samples with minimal amplification steps. Localised heating is a potential approach for improving nucleic hybridisation rates and reducing non-specific interactions, and thereby producing high sensitivity and selectivity [1-3]. The aim of the study was therefore to develop a microheater surrounding Au thin film electrodes, an integrated hybrid chip, for detecting genes of Mycobacterium tuberculosis with enhanced sensitivity. The performance of the integrated hybrid chip was determined using the changes in the charge transfer resistance (Rct) upon DNA hybridisation using probe sequences for Mycobacterium tuberculosis. Heat transfer within the system was simulated by using COMSOL Multiphysics as a mathematical modelling tool. When a temperature of 50 °C was applied to the microheater during DNA hybridisation steps, Rct values (which were indicative of DNA-DNA hybridisation) increased 236% and 90% as opposed to off-chip non-heated experiments and off-chip heated experiments. It is concluded from these observations that the microheater indeed can significantly improve the performance of the nucleic acid hybridisation assay and paves the way for the development of highly- sensitive and specific integrated label-free biosensors.

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“…In short, the signal change for SPEs were less than that of the hybrid device. Our results indicate similar characteristics with the literature [50].…”

Section: Electrochemical Performance Of Chip Under Ambient and Elevat...supporting

confidence: 92%

Section: Surface Characterization Of Spes and The Chipmentioning

confidence: 99%

An electrochemical biosensor with integrated microheater to improve the sensitivity of electrochemical nucleic acid biosensors

Akcakoca¹,

Ghorbanpoor²,

Blair³

et al. 2022

J. Micromech. Microeng.

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show abstract

“…Therefore, there is an urgent need to establish a sensitive, simple, and highly specific method for detecting E. coli O157: H7. At present, many different electrochemical sensors have been used in food safety due to their some advantages, such as high sensitivity, economy, and accuracy (Li et al, 2015b;Li et al, 2016;Dong et al, 2020). Therefore, electrochemical biosensors for the detection of E. coli O157:H7 have received extensive attention.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7

Lin

Mei

et al. 2021

Front. Chem.

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Escherichia coli (E. coli) O157:H7 can cause many food safety incidents, which seriously affect human health and economic development. Therefore, the sensitive, accurate, and rapid determination of E. coli O157:H7 is of great significance for preventing the outbreak and spread of foodborne diseases. In this study, a carbon dots-Fe3O4 nanomaterial (CDs-Fe3O4)-based sensitive electrochemical biosensor for E. coli O157:H7 detection was developed. The CDs have good electrical conductivity, and the surface of carbon dots contains abundant carboxyl groups, which can be used to immobilize probe DNA. Meanwhile, the CDs can be used as a reducing agent to prepare CDs-Fe3O4 nanomaterial. The Fe3O4 nanomaterial can improve the performance of the electrochemical biosensor; it also can realize the recovery of CDs-Fe3O4 due to its magnetism. As expected, the electrochemical biosensor has excellent specificity of E. coli O157:H7 among other bacteria. The electrochemical biosensor also exhibited good performance for detecting E. coli O157:H7 with the detection range of 10–108 CFU/ml, and the detection limit of this electrochemical biosensor was 6.88 CFU/ml (3S/N). Furthermore, this electrochemical biosensor was successfully used for monitoring E. coli O157:H7 in milk and water samples, indicating that this electrochemical biosensor has good application prospect. More importantly, this research can provide a new idea for the detection of other bacteria and viruses.

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“…Different analytical strategies have been proposed for H 2 O 2 detection such as chemiluminescence, [14] fluorescence, [15,16] and electrochemical techniques [17]. Among these, electrochemical sensors are very appealing for their simplicity, speed, sensitivity, miniaturization and cost-effectiveness [18]. In the past two decades biosensors have dominated the sensors scenario even in the ROS detection field [17,19], while in the last decade nanomaterials have become alternative/ complementary sensor useful tools [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson’s disease in vitro model

Rojas

Pelle

Carlo

et al. 2018

Sensors and Actuators B: Chemical

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In this work, we present the combination of Carbon Black (CB) and electrodeposited Prussian Blue (PB) covered with a Nafion layer on Screen-Printed electrodes (CB/PB-SPE) used for non-enzymatic H 2 O 2 sensing in Neuroblastoma cell line SH-SY5Y. These cells were challenged with 6hidroxidopamine (6-OHDA) for modelling Parkinson's disease. The electrodes surface was investigated using Scanning Electron Microscopy (SEM) and electrochemically characterized, in terms of electroactivity and stability. Electrochemical sensing of H 2 O 2 was carried out at very low potentials (-50mV), with a LOD of 0.01 μM and linear range between 0.2 and 1000 μM, allowing interference-free detection of H 2 O 2 in the selected cell culture. The H 2 O 2 concentration was successfully monitored in an experimental model of Parkinson's disease. These results pave the way to a method for the continuous monitoring of H 2 O 2 in culture medium for future studies of the role of H 2 O 2 in Parkinson's disease.

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The art of signal transforming: electrodes and their smart applications in electrochemical sensing

Abstract: Electrochemical biosensors were fabricated with electrode and recognition element and electrode was used to transform the recognition of a biological molecule into an easily quantifiable electrical signal.

Cited by 14 publications

References 175 publications

An electrochemical biosensor with integrated microheater to improve the sensitivity of electrochemical nucleic acid biosensors

An electrochemical biosensor with integrated microheater to improve the sensitivity of electrochemical nucleic acid biosensors

Electrochemical Biosensing Interface Based on Carbon Dots-Fe3O4 Nanomaterial for the Determination of Escherichia coli O157:H7

Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson’s disease in vitro model

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