The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development.

Prade, Rolf A.; Timberlake, William E.

doi:10.1002/j.1460-2075.1993.tb05898.x

Cited by 117 publications

(116 citation statements)

References 40 publications

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“…The 2.6-kb transcript markedly accumulated in various stages of growth and development as well as under the varying cultural conditions. In A. nidulans, the stuA and brlA genes, which are necessary for normal development of conidia, produce two distinctive transcripts that are, respectively, designated a and b (Miller et al 1992;Prade and Timberlake 1993). Their expression changes as conidiation proceeds.…”

Section: Discussionmentioning

confidence: 99%

ThensdCGene Encoding a Putative C2H2-Type Transcription Factor Is a Key Activator of Sexual Development inAspergillus nidulans

Kim¹,

Chae

Han

et al. 2009

Genetics

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The formation of the Aspergillus nidulans fruiting body is affected by a number of genetic and environmental factors. Here, the nsdC (never in sexual development) gene-encoding a putative transcription factor carrying a novel type of zinc-finger DNA-binding domain consisting of two C 2 H 2 's and a C 2 HC motif that are highly conserved in most fungi but not in plants or animals-was investigated. Two distinct transcripts of 2.6 and 3.0 kb were generated from nsdC. The 2.6-kb mRNA accumulated differentially in various stages of growth and development, while the level of the 3.0-kb mRNA remained relatively constant throughout the life cycle. While the deletion of nsdC resulted in the complete loss of fruiting body formation under all conditions favoring sexual development, overexpression of nsdC not only enhanced formation of fruiting bodies (cleistothecia) but also overcame inhibitory effects of certain stresses on cleistothecial development, implying that NsdC is a key positive regulator of sexual development. Deletion of nsdC also retarded vegetative growth and hyperactive asexual sporulation, suggesting that NsdC is necessary not only for sexual development but also for regulating asexual sporulation negatively. Overexpression of veA or nsdD does not rescue the failure of fruiting body formation caused by nsdC deletion. Furthermore, nsdC expression is not affected by either VeA or NsdD, and vice versa, indicating that NsdC regulates sexual development independently of VeA or NsdD.

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Section: Discussionmentioning

confidence: 99%

ThensdCGene Encoding a Putative C2H2-Type Transcription Factor Is a Key Activator of Sexual Development inAspergillus nidulans

Kim¹,

Chae

Han

et al. 2009

Genetics

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“…These mini-ORFs are probably not exons of 1998). BrlA is required to initiate conidiation Prade and Timberlake 1993). AbaA is re-REN1, because we could not find introns with consensus sequences for 5Ј and 3Ј splice signals to join mini-ORFs quired for phialide differentiation, and WetA is essential for maturation of conidia (Sewall et al 1990; Marand REN1 ORF as a single ORF.…”

Section: And B) B50-19 Lacked Normal Conidiophores and Phialides Andmentioning

confidence: 93%

REN1Is Required for Development of Microconidia and Macroconidia, but Not of Chlamydospores, in the Plant Pathogenic FungusFusarium oxysporum

et al. 2004

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The filamentous fungus Fusarium oxysporum is a soil-borne facultative parasite that causes economically important losses in a wide variety of crops. F. oxysporum exhibits filamentous growth on agar media and undergoes asexual development producing three kinds of spores: microconidia, macroconidia, and chlamydospores. Ellipsoidal microconidia and falcate macroconidia are formed from phialides by basipetal division; globose chlamydospores with thick walls are formed acrogenously from hyphae or by the modification of hyphal cells. Here we describe rensa, a conidiation mutant of F. oxysporum, obtained by restrictionenzyme-mediated integration mutagenesis. Molecular analysis of rensa identified the affected gene, REN1, which encodes a protein with similarity to MedA of Aspergillus nidulans and Acr1 of Magnaporthe grisea. MedA and Acr1 are presumed transcription regulators involved in conidiogenesis in these fungi. The rensa mutant and REN1-targeted strains lack normal conidiophores and phialides and form rod-shaped, conidium-like cells directly from hyphae by acropetal division. These mutants, however, exhibit normal vegetative growth and chlamydospore formation. Nuclear localization of Ren1 was verified using strains expressing the Ren1-green fluorescent protein fusions. These data strongly suggest that REN1 encodes a transcription regulator required for the correct differentiation of conidiogenesis cells for development of microconidia and macroconidia in F. oxysporum.

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“…Illumination for periods .4 hr and up to 12 hr did not result in photoinduction of brlA (data not shown). Two overlapping transcripts are produced from the brlA gene, where BrlAb activates the transcription of brlAa and subsequently BrlAa activates the conidiation genes (Prade and Timberlake 1993). To check whether any of these transcripts was preferentially regulated upon light exposure, primer sets specific for each transcript were used in the quantitative RT-PCR experiments ( Figure 2B).…”

Section: Identification Of Light-regulated Conidiation Genes In a Nimentioning

confidence: 99%

Regulation of Conidiation by Light in Aspergillus nidulans

et al. 2011

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Light regulates several aspects of the biology of many organisms, including the balance between asexual and sexual development in some fungi. To understand how light regulates fungal development at the molecular level we have used Aspergillus nidulans as a model. We have performed a genome-wide expression analysis that has allowed us to identify .400 genes upregulated and .100 genes downregulated by light in developmentally competent mycelium. Among the upregulated genes were genes required for the regulation of asexual development, one of the major biological responses to light in A. nidulans, which is a pathway controlled by the master regulatory gene brlA. The expression of brlA, like conidiation, is induced by light. A detailed analysis of brlA light regulation revealed increased expression after short exposures with a maximum after 60 min of light followed by photoadaptation with longer light exposures. In addition to brlA, genes flbA-C and fluG are also light regulated, and flbA-C are required for the correct lightdependent regulation of the upstream regulator fluG. We have found that light induction of brlA required the photoreceptor complex composed of a phytochrome FphA, and the white-collar homologs LreA and LreB, and the fluffy genes flbA-C. We propose that the activation of regulatory genes by light is the key event in the activation of asexual development by light in A. nidulans.

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The Aspergillus nidulans brlA regulatory locus consists of overlapping transcription units that are individually required for conidiophore development.

Cited by 117 publications

References 40 publications

ThensdCGene Encoding a Putative C2H2-Type Transcription Factor Is a Key Activator of Sexual Development inAspergillus nidulans

ThensdCGene Encoding a Putative C2H2-Type Transcription Factor Is a Key Activator of Sexual Development inAspergillus nidulans

REN1Is Required for Development of Microconidia and Macroconidia, but Not of Chlamydospores, in the Plant Pathogenic FungusFusarium oxysporum

Regulation of Conidiation by Light in Aspergillus nidulans

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