The assessment of cytokines in Quantiferon supernatants for the diagnosis of latent TB infection in a tribal population of Melghat, India

Bapat, Prachi R.; Husain, Aliabbas A.; Daginawala, Hatim F.; Agrawal, Neha; Panchbhai, Milind S.; Satav, Ashish; Taori, Girdhar M.; Kashyap, Rajpal S.

doi:10.1016/j.jiph.2015.02.003

Cited by 11 publications

(7 citation statements)

References 30 publications

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“…Whereas others confirmed the capability of IL-6 to detect M. tuberculosis in contacts of tuberculosis patients [28], own previous studies indicated a minor capacity of IL-6 in a tuberculosis low-endemic region [22]. IP-10, a potential biomarker described to improve sensitivity of IGRAs especially in children [16,29] and in HIV co-infection [30,31] detected low proportions of particularly HIV co-infected children.…”

Section: Discussionmentioning

confidence: 95%

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Lundtoft

Awuah

Nausch

et al. 2017

Med Microbiol Immunol

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IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON (QFT) supernatants can circumvent the IFN-γ-dependency and may improve QFT sensitivity. We aimed to identify additional cytokines from QFT supernatants for detection of Mycobacterium tuberculosis infection in children with tuberculosis and HIV co-infection from Ghana. Concentrations of 18 cytokines in QFT supernatants from children (0-16 years) with tuberculosis concomitantly infected with HIV (n = 25) or without HIV (n = 24) from Ghana were measured using cytometric bead array (CBA). 29% of the children showed positive IFN-γ test results, and five cytokines, i.e., IL-6, IL-21, TNF-α, IL-1α and IP-10, detected M. tuberculosis infection with comparable or, for IL-6, with significantly higher sensitivity (59%). Increased age and HIV co-infection were associated with decreased cytokine induction, and especially IL-21 and IP-10 were less prevalent in HIV co-infected children with tuberculosis. Combined cytokine analyses increased proportions of positive tests, and a four-cytokine subset (i.e., IL-6, IL-21, IFN-γ, IL-1α) predicted 78% of the children with tuberculosis correctly. Combined evaluation of IFN-γ and alternative cytokines improved IGRA-sensitivity in children with tuberculosis.

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Section: Discussionmentioning

confidence: 95%

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Lundtoft

Awuah

Nausch

et al. 2017

Med Microbiol Immunol

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“…The serum cytokines levels of severe HFMD, interleukin-1β (IL-1β), IL-2, IL-6, IL-8, IL-10, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) were determined by using the IMMULITE-1000 Immunoassay System (Siemens Healthcare Global), an automated microbead-based analyzer that makes use of chemiluminescent technology for analyte detection, which was performed according to the manufacturer’s instructions [20]. Serum cytokines levels of 50 mild EV-A71, and 50 CV-A16 HFMD cases, and 100 healthy individuals were also measured.…”

Section: Methodsmentioning

confidence: 99%

Clinical characteristics and managements of severe hand, foot and mouth disease caused by enterovirus A71 and coxsackievirus A16 in Shanghai, China

Cai

Wang

et al. 2019

BMC Infect Dis

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Background Hand, foot and mouth disease (HFMD) is a transmissible infectious disease caused by human enteroviruses (EV). Here, we described features of children with severe HFMD caused by EV-A71 or coxsackievirus A16 (CV-A16) in Shanghai, China. Methods Severe EV-A71 or CV-A16 caused HFMD children admitted to the Xinhua Hospital from January 2014 and December 2016, were recruited retrospectively to the study. Symptoms and findings at the time of hospitalization, laboratory tests, treatments, length of stay and residual findings at discharge were systematically recorded and analyzed. Results Of 19,995 children visited clinic service with probable HFMD, 574 children (2.87%) were admitted, 234 children (40.76%) were confirmed with EV-A71 (90/574) or CV-A16 (144/574) disease. Most (91.02%) of the patients were under 5 years. Initial clinical symptoms of EV-A71 and CV-A16 cases were: fever > 39 °C in 81 (90%) and 119 (82.63%), vomiting in 31 (34.44%) and 28 (19.44%), myoclonic twitching in 19 (21.11%) and 11(7.64%), startle in 21 (23.33%) and 20 (13.69%), respectively. Serum levels of interleukin-1β (IL-1β), IL-2, IL-6, IL-8, interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α) were significantly upregulated in severe HFMD subjects. Forty-seven children (20.08%) treated with intravenous gamma globulin (IVIG) showed decreased duration of illness episodes. All children were discharged without complications. Conclusions EV-A71 and CV-A16 accounted 40.76% of admitted HFMD during 2014 to 2016 in Xinhua Hospital. IVIG appeared to be beneficial in shortening the duration of illness episodes of severe HFMD.

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“…However, after stimulation, we observed that the secretion of IFN-γ and TNF-α by NK cells was remarkably decreased in ATB patients. In general, NK cells are not only the key effectors in the innate immune response to various infections (Bapat et al, 2015 ), but are also the effective regulators in innate and adaptive immune responses (Deng et al, 2014 ). In accordance with previous studies (Kim et al, 2015 ; Pan et al, 2015 ), our results indicate that the impaired cytokine production by NK cells may be an important factor in TB development.…”

Section: Discussionmentioning

confidence: 99%

Application of ImmunoScore Model for the Differentiation between Active Tuberculosis and Latent Tuberculosis Infection as Well as Monitoring Anti-tuberculosis Therapy

Zhou

Hou

et al. 2017

Front. Cell. Infect. Microbiol.

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Tuberculosis (TB) is a leading global public health problem. To achieve the end TB strategy, non-invasive markers for diagnosis and treatment monitoring of TB disease are urgently needed, especially in high-endemic countries such as China. Interferon-gamma release assays (IGRAs) and tuberculin skin test (TST), frequently used immunological methods for TB detection, are intrinsically unable to discriminate active tuberculosis (ATB) from latent tuberculosis infection (LTBI). Thus, the specificity of these methods in the diagnosis of ATB is dependent upon the local prevalence of LTBI. The pathogen-detecting methods such as acid-fast staining and culture, all have limitations in clinical application. ImmunoScore (IS) is a new promising prognostic tool which was commonly used in tumor. However, the importance of host immunity has also been demonstrated in TB pathogenesis, which implies the possibility of using IS model for ATB diagnosis and therapy monitoring. In the present study, we focused on the performance of IS model in the differentiation between ATB and LTBI and in treatment monitoring of TB disease. We have totally screened five immunological markers (four non-specific markers and one TB-specific marker) and successfully established IS model by using Lasso logistic regression analysis. As expected, the IS model can effectively distinguish ATB from LTBI (with a sensitivity of 95.7% and a specificity of 92.1%) and also has potential value in the treatment monitoring of TB disease.

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The assessment of cytokines in Quantiferon supernatants for the diagnosis of latent TB infection in a tribal population of Melghat, India

Cited by 11 publications

References 30 publications

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana

Clinical characteristics and managements of severe hand, foot and mouth disease caused by enterovirus A71 and coxsackievirus A16 in Shanghai, China

Application of ImmunoScore Model for the Differentiation between Active Tuberculosis and Latent Tuberculosis Infection as Well as Monitoring Anti-tuberculosis Therapy

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