In the healthcare sector, the job satisfaction of professionals and/or healthcare workers plays a profound role in their performance and is further reflected in the health and satisfaction of the patient. In recent times, the healthcare workers in Nigeria were not seen to be gratified with the organizational policies. The various industrial unions of the health care professionals have engaged in several industrial strike actions over the years in an attempt to demand for better remuneration, improved working conditions, increased staff strength, reduction of work overload, and the likes. This situation among the healthcare professional has become worrisome in Ogun State. This study assessed factors influencing job satisfaction of healthcare professionals in Ogun State Hospitals, Nigeria. The study was conducted with a sample of 729 healthcare professionals from the five zonal offices comprising Thirty-Nine (39) Health facilities across the study area. Data and/or items that assessed job satisfaction with management, supervision, nature of work/profession, work environment, salary, benefits, and promotion were subjected to exploratory factor analysis in order to group the major constraints variables. The results showed two orthogonal factors in management, two factors in supervision, five factors in nature of work/profession, three factors in a work environment, and three factors in rewards/compensation, which were derived with total explanations of 50.54%, 61.30%, 58.32%, 58.82%, and 68.68% respectively, of the Variance. Only variables with constraints loadings of 0.60 and above were used in naming the factors. The results further revealed that rewards, opportunities for career development, working conditions, and promotional schemes of the organizations have high associations with job satisfaction, whereas work environment was found to have low significance towards job satisfaction. This study concludes that the Ogun State Hospitals Management Board needs to improve the work environment; attractive rewards/compensation, opportunities for career development, and promotions. Keywords: Factors, Healthcare Professionals, Job satisfaction, Nigeria, Ogun State Hospitals.