The Association between Caffeine Intake and the Colonic Mucosa-Associated Gut Microbiota in Humans—A Preliminary Investigation

Abstract

We examined the association between caffeine and coffee intake and the community composition and structure of colonic microbiota. A total of 34 polyp-free adults donated 97 colonic biopsies. Microbial DNA was sequenced for the 16S rRNA gene V4 region. The amplicon sequence variant was assigned using DADA2 and SILVA. Food consumption was ascertained using a food frequency questionnaire. We compared the relative abundance of taxonomies by low (<82.9 mg) vs. high (≥82.9 mg) caffeine intake and by never or <2 cups vs. 2 cups vs. ≥3 cups coffee intake. False discovery rate-adjusted p values (q values) <0.05 indicated statistical significance. Multivariable negative binomial regression models were used to estimate the incidence rate ratio and its 95% confidence interval of having a non-zero count of certain bacteria by intake level. Higher caffeine and coffee intake was related to higher alpha diversity (Shannon index p < 0.001), higher relative abundance of Faecalibacterium and Alistipes, and lower relative abundance of Erysipelatoclostridium (q values < 0.05). After adjustment of vitamin B2 in multivariate analysis, the significant inverse association between Erysipelatoclostridium count and caffeine intake remained statistically significant. Our preliminary study could not evaluate other prebiotics in coffee.

Stress and depression-associated shifts in gut microbiota: A pilot study of human pregnancy

“…During the 2nd trimester, participants reporting moderate depressive symptoms had a lower abundance of Erysipelatoclostridium , as compared to those reporting low depressive symptoms. Erysipelatoclostridium are glucose, fructose, and sucrose-fermenting bacteria ( Yutin and Galperin, 2013 ) which have mostly been reported with diet-associated alterations: lower relative abundance of Erysipelatoclostridium has been implicated with increased caffeine consumption as well as folate and vitamin B2 intake in non-pregnant adults ( Dai et al, 2023 ; Gurwara et al, 2019 ). Of the few preliminary studies during pregnancy, most report on the infant fecal microbiome: Erysipelatoclostridium was more abundant in infants born to mothers with low fruit and vegetable consumption during pregnancy ( Fan et al, 2021 ) and was depleted in infants born to mothers with gestational diabetes ( Zhao et al, 2022 ).…”

Associations between gut microbiota and three prostate diseases: a bidirectional two-sample Mendelian randomization study

“…Eubacterium eligens group, a beneficial bacteria, significantly correlated positively with CD3 + T cells and negatively with NK cells, which may take a toll in the prevention of prostatitis 43 . Also, there is growing evidence showing the Erysipelatoclostridium plays in immune response, inflammation, and response to cancer immune therapy 44 . Methanobacteriaceae is the predominant methanogen in the human gut, and its presence or absence in the gastrointestinal tract has been linked to a variety of health characteristics, including changes in body weight and cardiovascular disease 45 .…”

Instituto de pesquisa café e saúde: Prevenção de doenças e melhora da qualidade de vida

“…Este benefício robusto e reprodutível está associado a um efeito hepatoprotetor do café (Heath et al, 2017), resultando numa diminuição da incidência de fígado gorduroso, fibrose hepática, cirrose e desenvolvimento de hepatocarcinomas em consumidores regulares de café (F. Liu et al, 2015;Marventano et al, 2016;Saab et al, 2014;Wijarnpreecha et al, 2017), em particular em doentes com hepatite C (Jaruvongvanich et al, 2017;Machado et al, 2014;Wijarnpreecha et al, 2017). Embora os mecanismos moleculares relacionados a esta hepatoproteção ainda não estejam explicados, provavelmente resultam de um efeito combinado de diferentes constituintes do café, especialmente das propriedades antioxidantes e anti-inflamatórias dos ácidos clorogênicos (Liang & Kitts, 2015;Naveed & Kubiliun, 2018;Tajik et al, 2017), e os efeitos da cafeína na fibrose, angiogênese e inflamação hepática (Alferink et al, 2018;Vaughn et al, 2012), eventualmente complementados pelo impacto benéfico do café no microbioma (Dai et al, 2023;Jaquet et al, 2009;Nehlig, 2022) e na atenuação da inflamação sistêmica (Agudelo-Ochoa et al, 2016;Martini et al, 2016;Zampelas et al, 2004).…”