The association between IFN-γ and IL-4 genetic polymorphisms and childhood susceptibility to bronchial asthma

Huang, Huarong; Zhong, Yingqiang; Wu, Jing

doi:10.1016/j.gene.2011.09.027

Cited by 22 publications

(13 citation statements)

References 24 publications

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“…In general, IFN-γ is considered as an inhibitory cytokine of IL-9 generation and Th9 cell differentiation. Down-regulation of IFN-γ in allergic asthma has been reported in previous studies [32, 33]. We also observed that the production and secretion of IFN-γ were decreased in children with allergic asthma.…”

Section: Discussionsupporting

confidence: 88%

Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients

Jia

Wang

et al. 2017

BMC Immunol

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BackgroundInterleukin-9 (IL-9) was reported as an active participant in the pathogenesis of allergic asthma. This study aimed to investigate the major source ofIL-9 and its effect on interferon γ (IFN-γ) and immunoglobulin (Ig) secretion by B cells.MethodsWe isolated peripheral blood mononuclear cells from children with allergic asthma and healthy children. IL-9, IL-4 and IFN-γ expression were detected by ELISA, ELISpot and Flowcytometry. Expression of transcription factor PU.1 was measured by Western Blot. We evaluated the effect of IL-9 on B cell activation and Ig production.ResultsResults showed that compared with healthy children, levels of IL-9, IL-4 and PU.1 were elevated and levels of IFN-γ were lower in children with allergic asthma. IL-9-expressing CD4+ T cells did not co-express IL-4. Exogenous IL-9 inhibited IFN-γ production in a dose-dependent manner. Antigen-specific Th9 cells existed in children with house dust mite allergic asthma. IL-9 up-regulated expression of CD69 and CD25 on B cells and combination of IL-9 and IL-4 enhanced IgE production.ConclusionsIn conclusion, our results showed that Th9 cells may be the major source of IL-9 in children with allergic asthma. In these patients, IL-9 impairs IFN-γ production and synergistically promotes IL-4-induced IgE secretion.

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Section: Discussionsupporting

confidence: 88%

Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients

Jia

Wang

et al. 2017

BMC Immunol

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“…The genotype distributions of these four polymorphisms in healthy subjects were similar to those observed in previous studies of Japanese populations [16–21]. Patients with cITP had a significantly lower frequency of the IL-4Rα 576 QQ genotype compared to healthy controls using a dominant model (69.8% vs. 79.7% respectively, odds ratio [OR] = 0.59, 95% confidence interval [CI] = 0.35–0.98, P = 0.04).…”

Section: Resultssupporting

confidence: 86%

The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP

et al. 2017

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BackgroundT-helper cell type 1 (Th1) polarization in chronic immune thrombocytopenia (cITP) has been reported at the protein and mRNA levels. We evaluated the impact of Th1/Th2 cytokine and cytokine receptor functional polymorphisms on both susceptibility to, and severity of, cITP. We analysed IFN-γ + 874 T/A, IFN-γR -611G/A, IL-4 -590C/T, and IL-4Rα Q576R polymorphisms in 126 cITP patients (male/female: 34/92; median age: 47.7 years) and 202 healthy control donors. Genotyping was determined by PCR and direct sequencing. The Th1/Th2 ratio was detected in peripheral blood mononuclear cells via flow cytometry.ResultscITP patients had a higher frequency of the IL-4Rα 576 non-QQ genotype compared to healthy subjects (P = 0.04). cITP patients with the IFN-γ +874 non-AA genotype (high expression type) showed more severe thrombocytopenia than those with the AA genotype (P < 0.05). cITP patients had a significantly higher Th1/Th2 ratio than control patients (P < 0.01); this ratio was inversely correlated with platelet counts. Furthermore, patients with both IFN-γ +874 non-AA genotype (high expression type) and IFN-γR −611 non-AA genotype (high-function type) had a significantly higher Th1/Th2 ratio (P < 0.05).ConclusionsThe cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP.

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“…It may be concluded that the IL-4 -590C>T polymorphism is associated with increased serum IgE level. Consistent with this, Huang et al (2012) showed that this C>T mutation at position -590 of the IL-4 promoter is related to increased total serum IgE in a case-control study of 122 control subjects and 100 asthmatic children from the Guangdong region of China. Furthermore, Russian researchers have shown that this substitution positively correlates with increased IgE levels in Russian asthma patients (Gervaziev et al, 2006).…”

Section: Discussionsupporting

confidence: 58%

Association between the interleukin 4 gene -590C>T promoter polymorphism and asthma in Xinjiang Uighur children

et al. 2016

Genet. Mol. Res.

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ABSTRACT. We investigated the association between the interleukin 4 gene (IL-4) -590C>T polymorphism and forced expiratory volume in one second (FEV 1 ) values, immunoglobulin E (IgE) levels, and susceptibility to asthma in Uighur children. IL-4 -590C>T frequencies were analyzed in 38 bronchial asthmatic patients and 35 non-asthmatic controls. Polymerase chain reaction and direct sequencing were applied to determine the residue at position -590 of IL-4. Total serum IgE levels were detected by enzyme-linked immunosorbent assay, while lung function was examined by professionals. There were significant differences in the distribution of IL-4 -590C>T genotypes and alleles between patient and control groups (genotypes: chi-square = 11.476, P < 0.05; alleles: chi-square = 14.572, P < 0.05). Frequencies of CC, CT, and TT genotypes were 21, 29, and 50% among patients, and 49, 37, and 14% among controls, respectively, indicating that the T allele was significantly more frequent in the asthma group than in the control group. Total serum IgE levels were significantly higher (P < 0.05) and FEV 1 values were significantly lower (F = 13.294, P < 0.05) in patients than in control subjects of the same genotype. In conclusion, the IL-4 -590C>T polymorphism is related to bronchial asthma in Uighur children, and the T allele may constitute a susceptibility factor in this group. Furthermore, this genetic variant can result in raised IgE levels and decreased FEV 1 values, suggesting that both factors are associated with bronchial asthma in Uighur children.

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The association between IFN-γ and IL-4 genetic polymorphisms and childhood susceptibility to bronchial asthma

Cited by 22 publications

References 24 publications

Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients

Detection of IL-9 producing T cells in the PBMCs of allergic asthmatic patients

The cytokine polymorphisms affecting Th1/Th2 increase the susceptibility to, and severity of, chronic ITP

Association between the interleukin 4 gene -590C>T promoter polymorphism and asthma in Xinjiang Uighur children

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