The Association between lasB and nanI Genes with Biofilm Formation in Pseudomonas aeruginosa Clinical Isolates

Mahdavi, Zahra; Hemati, Saeed; Sadeghifard, Nourkhoda; Jalilian, Farid Azizi; Taherikalani, Morovat; Bimanand, Lida; Pakzad, Iraj; Ghafourian, Sobhan

doi:10.7860/jcdr/2020/16076.13697

Cited by 4 publications

(4 citation statements)

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“…The genes encoding virulence factors ( algD , lasB , plcH , nan1 , exoS , and exoA ) and β‐lactamase resistance genes (ESBL genes [ bla CTXM , bla SHV , bla TEM ] and carbapenemase genes [ bla VIM , bla IMP , bla NDM , bla OXA‐48 , bla OXA‐23 , and bla OXA‐11 ]) were detected by PCR method using the specific primers (Table 1). 19–22 Then, 1% agarose gel electrophoresis and gel staining (stain load dye (CinnaGen Co, Iran)) were conducted for the analysis of PCR products.…”

Section: Methodsmentioning

confidence: 99%

“…The quality of the extracted DNA was determined using an absorbance ratio of 260/280 nm by a NanoDrop PCR method using the specific primers (Table 1). [19][20][21][22] Then, 1% agarose gel electrophoresis and gel staining (stain load dye (CinnaGen Co, Iran)) were conducted for the analysis of PCR products.…”

Section: Molecular Detection Of Virulence and Resistancementioning

confidence: 99%

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Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Ghasemian

Karami‐Zarandi

Heidari

et al. 2023

Clinical Laboratory Analysis

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Section: Methodsmentioning

confidence: 99%

Section: Molecular Detection Of Virulence and Resistancementioning

confidence: 99%

Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Ghasemian

Karami‐Zarandi

Heidari

et al. 2023

Clinical Laboratory Analysis

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“…Four different disc diffusion of antibiotics were used: ceftazidime (30), amikacin (10), imipenem (10) and colistin (10). Detection of the resistance, susceptible or intermediate isolates, relied on CLSI guideline [18].…”

Section: Antibiotic Susceptibility Testmentioning

confidence: 99%

“…Quorum sensing has a major role in bacterial motility in addition to expression of virulence factors, hence forming biofilm [17]. In P. aeruginosa lasB plays an important role in biofilm formation by acting as polysaccharide secretion regulator [18]. A study showed that about 1% from total genes in P. aeruginosa were enrolled with biofilm formation, among thesewere the las and rhl which are quorum-sensing genes that mediate biofilm formation [19].…”

Section: Introductionmentioning

confidence: 99%

Detecting the Variation in the lasI Gene and Their Relation with Biofilm in Pseudomonas aeruginosa Isolates

Al-Maeni

2024

Iraqi Journal of Science

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One of the most causative agents for many opportunistic diseases is the Pseudomonas aeruginosa which has a high percentage of multidrug resistance disease through construction of biofilm. The current study aimed for evaluating the correlation between quorum sensing genes (which is lasI gene) and biofilm formation. The biofilm construction and antibiotics susceptibility test were achieved for all the isolates under the study. The PCR and sequencing techniques were also carried out to detect the type of variation in lasI gene for each scheme of biofilm formation (weak, strong, and moderate). High antibiotic resistance was recorded among biofilm producing isolates. The genic pattern for the weak biofilm producer, which is isolate No 19, carries lysine instead of arginine in position 92 and aspartic acid instead of asparagine in position 115. Phylogenetic analysis indicated that the horizontal genetic transfer between isolates was very high. Finally, we conclude that the lasI gene does participate in biofilm construction of P. aeruginosa.

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Identification of blaOXA-23 gene in resistant Pseudomonas aeruginosa strains isolated from cows and humans in Basra province, Iraq

Jasim,

Mohammed,

Abbas

et al. 2024

Vet World

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Background and Aim: Pseudomonas aeruginosa is an infectious agent of great importance for animals and humans. It causes serious infections that show high resistance to antibiotics. This study investigated the molecular detection of blaOXA-23 gene in antibiotic-resistant P. aeruginosa strains isolated from cows and humans. Materials and Methods: In total, 120 samples, comprised 60 from cows (30 milk and 30 nasal discharge) and 60 from their owners (30 urine and 30 sputum), were individually collected, cultured, and tested for P. aeruginosa through molecular analysis targeting the blaOXA-23 gene. P. aeruginosa antibiotic-resistant isolates were identified by performing antibiotic susceptibility testing and detecting biofilm formation. Results: In total, 74.17% positive P. aeruginosa isolates, including 66.67% and 81.67% for cows and humans, respectively. Subsequently, positive cow isolates were detected in 60% of milk samples and 73.33% of nasal discharge samples; while positive human isolates were detected in 76.67% of urine samples and 86.66% of sputum samples. Targeting blaOXA-23 gene, 58.43% of cultured isolates were positive for P. aeruginosa by polymerase chain reaction. Respectively, positive isolates were detected in 66.67% and 45.46% of cow milk and nasal discharges as well as in 60.87% and 61.54% of human urine and sputum. The antibiotic susceptibility test revealed that all isolates were resistant to all applied antibiotics, particularly imipenem. Results of biofilm formation revealed 67.31% total positives, including 51.43% strong, 34.285% moderate, and 14.285% weak reactions. In addition, although values of the total positive cows and humans differed insignificantly, total positives showed insignificant variation between values of milk and nasal discharges of cows as well as between urine and sputum of humans; however, significant differences were identified in the distribution of strong, moderate, and weak positivity of these samples. Conclusion: Antibiotic overuse contributes extensively to increasing the prevalence of resistant P. aeruginosa isolates carrying the blaOXA-23 gene in both cows and humans. Furthermore, studies in other Iraqi areas are necessary to support our findings. The main limitations include that the number of tested samples is relatively low, and there is a need to use a large number of samples from different sources. Also, the current methods for detection of resistant isolates are still culture-based approaches. Keywords: antibiotic susceptibility test, biofilm formation, blaOxacillinases-23, conventional polymerase chain reaction, cow milk, nasal discharge, sputum, urine.

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The Association between lasB and nanI Genes with Biofilm Formation in Pseudomonas aeruginosa Clinical Isolates

Cited by 4 publications

References 0 publications

Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Molecular characterizations of antibiotic resistance, biofilm formation, and virulence determinants of Pseudomonas aeruginosa isolated from burn wound infection

Detecting the Variation in the lasI Gene and Their Relation with Biofilm in Pseudomonas aeruginosa Isolates

Identification of blaOXA-23 gene in resistant Pseudomonas aeruginosa strains isolated from cows and humans in Basra province, Iraq

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