The association of [131I]iodo-insulin with rat diaphragm muscle and its effect on glucose uptake

Garratt, Clifford J.; Cameron, J. S.; Menzinger, G.

doi:10.1016/0304-4165(66)90062-6

“…It is of interest in this connection that Garratt and coworkers (Garratt, Cameron andMenzinger 1966, Garratt, Jarrett andKeen 1966) have shown an association of 131 I labelled insulin to the diaphragm muscle over and above the quantity required to achieve maximal glucose uptake. It is of interest in this connection that Garratt and coworkers (Garratt, Cameron andMenzinger 1966, Garratt, Jarrett andKeen 1966) have shown an association of 131 I labelled insulin to the diaphragm muscle over and above the quantity required to achieve maximal glucose uptake.…”

Section: Duration Of the Insulin Effectmentioning

confidence: 99%

Glucose Metabolism and Response to Insulin of Isolated Fat Cells and Epididymal Fat Pads

Gliemann¹

1968

Acta Physiologica Scandinavica

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GLIEMANN, J. Glucose metabolism and response to insulin of isolated fat cells and epididymal fat pads. Acta physiol. scand. 1968. 72. 481-491. The glucose uptake and the conversion of glucose 1_ 14 C to 14C-carbon dioxide and 14C-tri_ glyceride by epididymal fat pads and fat cells isolated after treatment with collagenase was studied with varying concentrations of glucose in the incubation medium. The isolated fat cells took up and metabolized slightly more glucose than the fat pads at a low concentration (0.1 mg/ml) but several fold more than the fat pads at a high concentration (1.6 mg/ml). In the presence of insulin, the isolated cells took up and metabolized 2-3 times more glucose than the fat pads at the low medium glucose concentration and about the same as the fat pads at the high medium glucose concentration. The isolated cells were about 10 times more sensitive to insulin than the fat pads. The isolated cells responded to a concentration of 1.25~U of insulin per ml and a maximal effect was obtained with a concentration of 20~U per m!. The equivalent figures for the epididymal fat pads were about 10 ",U per ml and 320~U per m!.Isolated fat cells, preincubated with insulin (100~U/ml) and then washed, were stimulated for 15-30 min whereas epididymal fat pads, similarly preincubated with insulin (1,000~U/ ml) and washed, remained fully stimulated throughout a 2 hr incubation period. The differences in glucose metabolism of isolated fat cells and epididymal fat pads could be partially explained by a limitation of the glucose diffusion into the fat pad. In addition, the isolated cells seemed more permeable to glucose than fat cells of the "intact" tissue.

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“…A high concentration of insulin in the interstitial fluid of the tissue may explain the difference between the duration of the insulin effect on isolated fat cells and epididymal fat pads. It is of interest in this connection that Garratt and coworkers (Garratt, Cameron andMenzinger 1966, Garratt, Jarrett andKeen 1966) have shown an association of 131 I labelled insulin to the diaphragm muscle over and above the quantity required to achieve maximal glucose uptake. They suggested that inactively bound insulin might serve as a depot.…”

Section: Duration Of the Insulin Effectmentioning

confidence: 99%

Glucose Metabolism and Response to Insulin of Isolated Fat Cells and Epididymal Fat Pads

Gliemann

¹

1968

Acta Physiologica Scandinavica

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GLIEMANN, J. Glucose metabolism and response to insulin of isolated fat cells and epididymal fat pads. Acta physiol. scand. 1968. 72. 481-491. The glucose uptake and the conversion of glucose 1_ 14 C to 14C-carbon dioxide and 14C-tri_ glyceride by epididymal fat pads and fat cells isolated after treatment with collagenase was studied with varying concentrations of glucose in the incubation medium. The isolated fat cells took up and metabolized slightly more glucose than the fat pads at a low concentration (0.1 mg/ml) but several fold more than the fat pads at a high concentration (1.6 mg/ml). In the presence of insulin, the isolated cells took up and metabolized 2-3 times more glucose than the fat pads at the low medium glucose concentration and about the same as the fat pads at the high medium glucose concentration. The isolated cells were about 10 times more sensitive to insulin than the fat pads. The isolated cells responded to a concentration of 1.25~U of insulin per ml and a maximal effect was obtained with a concentration of 20~U per m!. The equivalent figures for the epididymal fat pads were about 10 ",U per ml and 320~U per m!.Isolated fat cells, preincubated with insulin (100~U/ml) and then washed, were stimulated for 15-30 min whereas epididymal fat pads, similarly preincubated with insulin (1,000~U/ ml) and washed, remained fully stimulated throughout a 2 hr incubation period. The differences in glucose metabolism of isolated fat cells and epididymal fat pads could be partially explained by a limitation of the glucose diffusion into the fat pad. In addition, the isolated cells seemed more permeable to glucose than fat cells of the "intact" tissue.

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Metabolic Effects on Muscular Tissue

Clausen¹

1975

Insulin

2

0

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The association of [131I]iodo-insulin with rat diaphragm muscle and its effect on glucose uptake

Cited by 23 publications

References 11 publications

Glucose Metabolism and Response to Insulin of Isolated Fat Cells and Epididymal Fat Pads

Glucose Metabolism and Response to Insulin of Isolated Fat Cells and Epididymal Fat Pads

Glucose Metabolism and Response to Insulin of Isolated Fat Cells and Epididymal Fat Pads

Metabolic Effects on Muscular Tissue

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