The Association of Lipoproteins With the Inhibition of Streptolysin S by Serum 1

Stollerman, Gene H.; Bernheimer, Alan W.; MacLeod, Colin M.

doi:10.1172/jci102408

Cited by 24 publications

(19 citation statements)

References 16 publications

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“…No hemolytic activity was found associated with the small amount of UV-absorbing material eluted at pH 9.8 or at pH 11.0, but streptolysin-inhibiting material was found in the pH 9.8 eluate (/3 lipoprotein). These associations with ~ and/3 lipoproteins are in accord with the observations of Humphrey (10) and of Stollerman et al (11).…”

Section: Chromatography Of "Serum Hemolysin" On Deae Cellulose and Onsupporting

confidence: 92%

Oxygen-Stable Hemolysins of Group a Streptococci

Ginsburg

Harris

1963

The Journal of Experimental Medicine

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In the preceding paper (1) the materials and methods involved in the production of oxygen-stable hemolysins by Group A streptococci were described.It was shown that although a variety of unrelated materials such as RNA, serum albumin, serum a lipoprotein, tween 40, and triton X-205 could induce the production of hemolysin from resting streptococci, a number of similarities could be demonstrated among the resulting preparations of hemolysin. All were inhibited by lecithin, trypan blue, congo red, papain, chymotrypsin, and by a/~ lipoprotein fraction of human serum, and none were affected by cholesterol or trypsin. On the other hand, differences were shown among the hemolysins induced by RNA, on one hand, and albumin, tween, and triton on the other. Thus, it was shown that the rates of production of the RNA and albumin hemolysins differ in both resting and growing streptococci, and that glucosamine, which is an excellent energy source for the production of RNA hemolysin, is not effective for the formation of the albumin or detergent hemolysin.These observations raised the question of whether we were dealing with different hemolysins, or with the same hemolysin produced by different metabolic pathways, or with a single hemolysin associated with various inducing agents. The purpose of the present study was to explore further the relationship within this group of hemolytic agents by chromatography, by electrophoresis, and by procedures which could inactivate preparations of the hemolysin produced by individual members of this group of inducing agents. Materials and MethodsThe Streptococcal strain and culture medium were described in the preceding paper (1).

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Section: Chromatography Of "Serum Hemolysin" On Deae Cellulose and Onsupporting

confidence: 92%

Oxygen-Stable Hemolysins of Group a Streptococci

Ginsburg

Harris

1963

The Journal of Experimental Medicine

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“…Streptolysin S has been shown to be inhibited by serum lipoproteins. This inhibition may be varied by measures which alter the native state of these proteins or by diseases which are associated with disturbances in lipid metabolism (4)(5)(6)(7)(8). Also, the inhibition of saponin hemolysis by whole plasma is not a simple function of the concentration of its lipid components.…”

mentioning

confidence: 99%

The Use of Hemolysin Inhibition in the Study of Experimental And Clinical Hyperlipemia: THE NON-SPECIFIC INHIBITION OF STREPTOLYSIN O BY SERUM LIPOPROTEINS1

Stollerman¹

1954

J. Clin. Invest.

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“…Previous studies indicated that the inhibition of this streptococcal hemolysin by serum is associated with alpha and beta lipoproteins rather than with specific antibody (2)(3)(4)(5). Streptolysin S is inhibited by saline suspensions of phospholipids but not by cholesterol or neutral fats (6,3). In addition, a large proportion of the SSI is destroyed by the action of Cl.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, a large proportion of the SSI is destroyed by the action of Cl. welchii lecithinase on serum (3). It therefore seemed possible that variation in SSI might reflect a parallel variation of serum lipoproteins rich in phospholipid.…”

Section: Introductionmentioning

confidence: 99%