The asymmetric, rectifier-like I–V curve of the Na/K pump transient currents in frog skeletal muscle fibers

Chen, Wei; Wu, Wen-Hui

doi:10.1016/s1567-5394(02)00046-4

Cited by 12 publications

(14 citation statements)

References 9 publications

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“…Previous studies demonstrated that stimulation by a train of squared pulses increase the pump current of skeletal muscle fibers [27], [29]. We further increased the field oscillating frequency in a stepwise pattern and found the stepwise up-regulation of the pump current [23].…”

Section: Discussionmentioning

confidence: 50%

“…As a result, the function of Na/K pump can be regulated by the oscillating EFs. The direct evidence is that the activation of the pump by this synchronization modulation hyperpolarizes the resting membrane potential of skeletal muscle fibers [23], [27], [29] and cardiomyocytes [26], [30].…”

Section: Discussionmentioning

confidence: 99%

“…The pump activity maintains transmembrane ionic gradients and cell membrane potentials that regulate cell volumes and generate driving forces to secondary active transport of other substances across the cell membrane. The voltage-dependent activation of this molecule has been extensively studied [29], [51]–[53]. Various electric fields (EFs) were also applied to stimulate the pump function [54]–[60].…”

Section: Discussionmentioning

confidence: 99%

“…Then, by gradually changing the field oscillating frequency, these pump molecules can be entrained to a defined value of the pumping rate. The technique has been successfully applied to skeletal muscle fibers [27], [29], cardiomyocyte [26], [30], cultured cell line, blood vessels, and kidney proximal tubules. The results showed that the synchronization modulation technique can effectively activate the pumping rate up to ten fold quickly in tens of seconds.…”

Section: Introductionmentioning

confidence: 99%

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Synchronization Modulation Increases Transepithelial Potentials in MDCK Monolayers through Na/K Pumps

et al. 2013

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Transepithelial potential (TEP) is the voltage across a polarized epithelium. In epithelia that have active transport functions, the force for transmembrane flux of an ion is dictated by the electrochemical gradient in which TEP plays an essential role. In epithelial injury, disruption of the epithelial barrier collapses the TEP at the wound edge, resulting in the establishment of an endogenous wound electric field (∼100 mV/mm) that is directed towards the center of the wound. This endogenous electric field is implicated to enhance wound healing by guiding cell migration. We thus seek techniques to enhance the TEP, which may increase the wound electric fields and enhance wound healing. We report a novel technique, termed synchronization modulation (SM) using a train of electric pulses to synchronize the Na/K pump activity, and then modulating the pumping cycles to increase the efficiency of the Na/K pumps. Kidney epithelial monolayers (MDCK cells) maintain a stable TEP and transepithelial resistance (TER). SM significantly increased TEP over four fold. Either ouabain or digoxin, which block Na/K pump, abolished SM-induced TEP increases. In addition to the pump activity, basolateral distribution of Na/K pumps is essential for an increase in TEP. Our study for the first time developed an electrical approach to significantly increase the TEP. This technique targeting the Na/K pump may be used to modulate TEP, and may have implication in wound healing and in diseases where TEP needs to be modulated.

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Section: Discussionmentioning

confidence: 50%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Synchronization Modulation Increases Transepithelial Potentials in MDCK Monolayers through Na/K Pumps

et al. 2013

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“…Recent evidence suggests that the ER may represent a critical organelle for the induction and regulation of apoptosis. Under stress conditions, such as age‐associated oxidative damage to the Ca 2+ ATPase pump, ER can induce cytochrome c release from mitochondria, thus initiating apoptosis in muscle cells . In addition, ER can release procaspase‐12, which, upon activation by m‐calpain, cleaves and activates caspase‐3 .…”

Section: Apoptosis Wound Healing and Agementioning

confidence: 99%

Influence of age on wound healing and fibrosis

Kapetanaki

Mora

Rojas

2012

The Journal of Pathology

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The incidence and severity of fibrotic lung diseases increase with age, but very little is known about how agerelated changes affect the mechanisms that underlie disease emergence and progression. Normal ageing includes accumulation of DNA mutations, oxidative and cell stresses, mitochondria dysfunction, increased susceptibility to apoptosis, telomere length dysfunction and differential gene expression as a consequence of epigenetic changes and miR regulation. These inevitable ageing-related phenomena may cause dysfunction and impaired repair capacity of lung epithelial cells, fibroblasts and MSCs. As a consequence, the composition of the extracellular matrix changes and the dynamic interaction between cells and their environment is damaged, resulting ultimately in predisposition for several diseases. This review summarizes what is known about age-related molecular changes that are implicated in the pathobiology of lung fibrosis in lung tissue.

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Electrical Activation of Na/K Pumps Can Increase Ionic Concentration Gradient and Membrane Resting Potential

Chen

Dando

2006

J Membrane Biol

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It has been previously demonstrated by our group that our specifically designed synchronization modulation electric field can dynamically entrain the Na/K ATPase molecules, effectively accelerating the pumping action of these molecules. The ATPase molecules are first synchronized by the field, and subsequently their pumping rates are gradually modulated in a stepwise pattern to progressively higher and higher levels. Here, we present results obtained on application of the field to intact twitch skeletal muscle fibers. The ionic concentration gradient across the cell membrane was monitored, with the membrane potential extrapolated using a slow fluorescent probe with a confocal microimaging technique. The applied synchronization-modulation electric field is able to slowly but consistently increase the ionic concentration gradient across the membrane and, hence, hyperpolarize the membrane potential. All of these results were fully eliminated if ouabain was applied to the bathing solution, indicating a correlation with the action of the Na/K pump molecules. These results in combination with our previous results into the entrainment of the pump molecules show that the synchronization-modulation electric field-induced activation of the Na/K pump functions can effectively increase the ionic concentration gradient and the membrane potential.

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The asymmetric, rectifier-like I–V curve of the Na/K pump transient currents in frog skeletal muscle fibers

Cited by 12 publications

References 9 publications

Synchronization Modulation Increases Transepithelial Potentials in MDCK Monolayers through Na/K Pumps

Synchronization Modulation Increases Transepithelial Potentials in MDCK Monolayers through Na/K Pumps

Influence of age on wound healing and fibrosis

Electrical Activation of Na/K Pumps Can Increase Ionic Concentration Gradient and Membrane Resting Potential

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