The Attenuation of 14-3-3ζ is Involved in the Caffeic Acid-Blocked Lipopolysaccharide-Stimulated Inflammatory Response in RAW264.7 Macrophages

Lu, Ming; Dai, Yi; Xu, Mingsheng; Zhang, Chi; Ma, Yuhong; Gao, Ping; Teng, Mengying; Jiao, Kailin; Huang, Guangming; Zhang, Jianping; Yang, Ye; Chu, Zhiping

doi:10.1007/s10753-017-0618-1

Cited by 20 publications

(6 citation statements)

References 39 publications

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“…F4/80 is a specific antigen for macrophages (36)(37)(38). iNOS is an important pro-inflammatory mediator expressed by macrophages under LPS induction (39)(40)(41). Thus, positive staining of both F4/80 and iNOS indicates macrophages are activated and are able to produce high levels of cytokines.…”

Section: Gent Attenuated M1 Type Macrophage Infiltration In Mouse Lunmentioning

confidence: 99%

Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway

Wang¹,

Zhou²,

Yang³

et al. 2019

Ann Transl Med

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Background: Sepsis is a high-mortality disease without effective therapeutic options. The hyperactivation of the monocyte-macrophage system, especially M1 macrophages, triggers the onset of septic shock. Gentiopicroside (GENT), the main active component in the traditional Chinese medicinal herb Radix Gentianae, has been shown to have anti-inflammatory properties. Nevertheless, this anti-inflammatory effect has not been fully elucidated.Methods: In vitro, we stimulated primary bone marrow-derived macrophages (BMMs) or peritoneal elucidated macrophages (PEMs) by lipopolysaccharide (LPS) and interferon (IFN)-γ and pre-treated with GENT and we tested the cytokines such as interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF) α production by enzyme linked immunosorbent assay (ELISA) or real-time quantitative PCR (qPCR). Further, we determined the NF-κB-mediated inflammatory pathway such as IKKα/β and p65 phosphorylation by Western blot. Then we detected the p65 nuclear localization by immunofluorescent staining. Moreover, NF-κB inhibitor and p65-targeted siRNAs were further used to validate the anti-inflammatory mechanism of GENT. In vivo, GENT (50 mg/kg) was administered intragastrically before and after LPS (40 mg/kg) injection. The death time were recorded and the serum levels of IL-1β, IL-6 and TNFα were tested by ELISA, and the IL-1β, IL-6 and TNFα mRNA expression in the lung were test by qPCR and the M1 infiltration in the lung were determined by F4/80 and INOS immunofluorescent staining Results: In vitro, we observed that GENT reduced the inflammatory cytokine production of BMMs stimulated by (LPS)/IFN-γ and ameliorated the phosphorylation of IKKα/β and p65, the degradation of IκBα, and the translocation of p65 into the nucleus. We did not find GENT has any effect on MAPK signaling under LPS/IFN-γ stimulation. NF-κB inhibitor and p65 siRNAs eliminated the inhibition effect of GENT. In vivo, we observed GENT prevented mice from dying in the LPS-induced shock model and decreased the serum levels of IL-1β and IL-6, the mRNA expression of IL-1β, IL-6 and TNFα in lung tissue, and the amount of M1 macrophage infiltration in the lung.Conclusions: GENT prevented LPS/IFN-γ-induced inflammatory cytokine production by macrophages through the NF-κB signaling pathway in vitro and protected against the endotoxin shock induced by LPS in vivo.

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Section: Gent Attenuated M1 Type Macrophage Infiltration In Mouse Lunmentioning

confidence: 99%

Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway

Wang¹,

Zhou²,

Yang³

et al. 2019

Ann Transl Med

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“…To confirm the effect of NM on macrophage activation in the liver, double immunofluorescence staining was performed to label the M1-specific antigen F4/80 and iNOS in liver sections [ 31 ]. The results demonstrated abundant double-positive macrophages in the livers of septic mice, whereas NM reduced their presence ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Neutrophil membrane-mimicking nanodecoys with intrinsic anti-inflammatory properties alleviate sepsis-induced acute liver injury and lethality in a mouse endotoxemia model

Xiao¹,

Ren

Gan³

et al. 2022

Materials Today Bio

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“…Previous studies showed that protocatechuic acid may inhibit the LPS-stimulated inflammatory mediator production in keratinocytes by reducing the Toll-like receptor-4-dependent activation of AKT, mTOR, and NF- κ B pathways and the activation of JNK and p38-MAPK [ 58 ]. Caffeic acid inhibited the inflammatory response by downregulating the phosphorylation of several important transcriptional factors, such as NF- κ B and STAT-3 [ 59 ]. In addition, vanillic acid inhibited the LPS-induced production of tumor necrosis factor TNF- α and interleukin IL-6 by suppressing the activation of NF- κ B and caspase-1 [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Network Pharmacology‐Based Identification of Potential Targets of Lonicerae japonicae Flos Acting on Anti‐Inflammatory Effects

Guo

Xiao²,

Zheng

et al. 2021

BioMed Research International

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Lonicerae japonicae flos (LJF) is widely used for the treatment of inflammation-related diseases in traditional Chinese medicine (TCM). To clarify the anti-inflammatory mechanism of LJF, 29 compounds with high content in LJF were selected for network pharmacology. Then, a comprehensive network pharmacology strategy was implemented, which involved compound-inflammation-target construction, protein-protein interaction (PPI) network analysis, and enrichment analysis. Finally, molecular docking and in vitro experiments were performed to verify the anti-inflammatory activity and targets of the key compound. As a result, 279 inflammation-associated proteins were identified, which are mainly involved in the AGE/RAGE signaling pathway in diabetic complications, the HIF-1 signaling pathway, the PI3K-AKT signaling pathway, and EGFR tyrosine kinase inhibitor resistance. A total of 12 compounds were linked to more than 35 targets, including apigenin, kaempferol, quercetin, luteolin, and ferulic acid. The results of molecular docking showed that AKT has the most binding activity, exhibiting certain binding activity with 10 compounds, including vanillic acid, protocatechuic acid, secologanic acid, quercetin, and luteolin; the results of qRT-PCR and WB confirmed that two key compounds, secologanic acid and luteolin, could significantly decrease the secretion of TNF-α and the AKT expression of RAW264.7 murine macrophages stimulated by LPS (lipopolysaccharide). These results demonstrate that the comprehensive strategy can serve as a universal method to illustrate the anti-inflammatory mechanisms of traditional Chinese medicine by identifying the pathways or targets.

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The Attenuation of 14-3-3ζ is Involved in the Caffeic Acid-Blocked Lipopolysaccharide-Stimulated Inflammatory Response in RAW264.7 Macrophages

Cited by 20 publications

References 39 publications

Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway

Gentiopicroside (GENT) protects against sepsis induced by lipopolysaccharide (LPS) through the NF-κB signaling pathway

Neutrophil membrane-mimicking nanodecoys with intrinsic anti-inflammatory properties alleviate sepsis-induced acute liver injury and lethality in a mouse endotoxemia model

Network Pharmacology‐Based Identification of Potential Targets of Lonicerae japonicae Flos Acting on Anti‐Inflammatory Effects

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