The attenuation of cockroach allergy by DNA vaccine encoding cockroach allergen Bla g 2

Zhou, Bin; Yuan, Jingdong; Zhou, Yixuan; Yang, Jun; James, Alan W.; Nair, Usha; Shu, Xiji; Liu, Wei; Kanangat, Siva; Yoo, T.J.

doi:10.1016/j.cellimm.2012.05.015

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…Since complete avoidance of cockroach allergen in the environment is extremely difficult, allergen-specific immunotherapy remains the only way to cure cockroach allergy. 39 An asthma murine model for cockroach allergy has been developed in our laboratory, and hopefully we will be able to use Per a 2-derived peptides to treat cockroach allergy using this murine model.…”

Section: Discussionmentioning

confidence: 99%

IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Lee

Chang

Song

et al. 2015

Allergy Asthma Immunol Res

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PurposeCockroaches are the second leading allergen in Taiwan. Sensitization to Per a 2, the major American cockroach allergen, correlates with clinical severity among patients with airway allergy, but there is limited information on IgE epitopes and tissue localization of Per a 2. This study aimed to identify Per a 2 linear IgE-binding epitopes and its distribution in the body of a cockroach.MethodsThe cDNA of Per a 2 was used as a template and combined with oligonucleotide primers specific to the target areas with appropriate restriction enzyme sites. Eleven overlapping fragments of Per a 2 covering the whole allergen molecule, except 20 residues of signal peptide, were generated by PCR. Mature Per a 2 and overlapping deletion mutants were affinity-purified and assayed for IgE reactivity by immunoblotting. Three synthetic peptides comprising the B cell epitopes were evaluated by direct binding ELISA. Rabbit anti-Per a 2 antibody was used for immunohistochemistry.ResultsHuman linear IgE-binding epitopes of Per a 2 were located at the amino acid sequences 57-86, 200-211, and 299-309. There was positive IgE binding to 10 tested Per a 2-allergic sera in 3 synthetic peptides, but none in the controls. Immunostaining revealed that Per a 2 was localized partly in the mouth and midgut of the cockroach, with the most intense staining observed in the hindgut, suggesting that the Per a 2 allergen might be excreted through the feces.ConclusionsInformation on the IgE-binding epitope of Per a 2 may be used for designing more specific diagnostic and therapeutic approaches to cockroach allergy.

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Section: Discussionmentioning

confidence: 99%

IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Lee

Chang

Song

et al. 2015

Allergy Asthma Immunol Res

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“…Purified cockroach allergens have been used in experimental mouse models for prophylaxis or treatment of cockroach-induced allergic lung inflammation [142, 143]. A DNA vaccine-encoding Bla g 2 was shown to be effective in suppressing airway inflammation and inducing a Th1 response, while suppressing a Th2 reaction, when used prior to sensitization in mice [142].…”

Section: Cockroach Allergens For Diagnosis and Therapymentioning

confidence: 99%

“…A DNA vaccine-encoding Bla g 2 was shown to be effective in suppressing airway inflammation and inducing a Th1 response, while suppressing a Th2 reaction, when used prior to sensitization in mice [142]. Cockroach sensitized mice, treated with liposome-entrapped natural Per a 9 (arginine kinase) via the intranasal route, presented significantly reduced respiratory tract inflammation after provocation.…”

Section: Cockroach Allergens For Diagnosis and Therapymentioning

confidence: 99%

Investigating cockroach allergens: Aiming to improve diagnosis and treatment of cockroach allergic patients

Pomés

Arruda

2014

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Cockroach allergy is an important health problem associated with the development of asthma, as a consequence of chronic exposure to low levels of allergens in susceptible individuals. In the last 20 years, progress in understanding the disease has been possible, thanks to the identification and molecular cloning of cockroach allergens and their expression as recombinant proteins. Assays for assessment of environmental allergen exposure have been developed and used to measure Bla g 1 and Bla g 2, as markers of cockroach exposure. IgE antibodies to cockroach extracts and to specific purified allergens have been measured to assess sensitization and analyze association with exposure and disease. With the development of the field of structural biology and the expression of recombinant cockroach allergens, insights into allergen structure, function, epitope mapping and allergen-antibody interactions have provided further understanding of mechanisms of cockroach allergic disease at the molecular level. This information will contribute to develop new approaches to allergen avoidance and to improve diagnosis and therapy of cockroach allergy.

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Optimization of a Der p 2-based prophylactic DNA vaccine against house dust mite allergy

et al. 2013

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The attenuation of cockroach allergy by DNA vaccine encoding cockroach allergen Bla g 2

Cited by 4 publications

References 24 publications

IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

IgE-Binding Epitope Mapping and Tissue Localization of the Major American Cockroach Allergen Per a 2

Investigating cockroach allergens: Aiming to improve diagnosis and treatment of cockroach allergic patients

Optimization of a Der p 2-based prophylactic DNA vaccine against house dust mite allergy

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