The Attenuation of the Virus of Epizootic Hemorrhagic Disease of Deer by Its Serial Passage in the Brains of Newborn Mice

Shope, Richard E.; MacNamara, Lester G.; Mettler, Norma E.

doi:10.1084/jem.118.3.421

Cited by 5 publications

(7 citation statements)

References 2 publications

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“…On the basis of previous reports by others (7,8,15,21,22), as well as the present study on the development of the virus, it seems probable that EHD virus is a RNA virus. Our preliminary results with acridine orange staining are that EHD virus infected BHK-21 cells developed applegreen fluorescent inclusions in the cytoplasm, thus suggesting that EHD virions may contain double-stranded ribonucleic acid.…”

Section: Cl~allenge Experiments and Ne~itralization Testssupporting

confidence: 83%

“…(i) The attenuated, mouse brain adapted EHD virus used in the present study was capable of inducing immunity in a deer which survived challenge with virulent EHD virus, a challenge which produced typical signs of EHD and death in a control deer. Shope et al (22) tissues, were observed in the endothelium of brain blood vessels of a deer intracerebrally inoculated with virulent EHD-infected deer spleen suspension. (iii) Serum from a deer which survived two injections of virulent EHD virus was effective in neutralizing the infectivity of the mouse-adapted EHD virus isolate, which was used in this study.…”

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

“…The EHD virus has been adapted and propagated in the newborn mouse brain in several laboratories (22,30). The mouse-adapted virus rapidly loses its virulence for deer (22).…”

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

“…The mouse-adapted virus rapidly loses its virulence for deer (22). HeLa cells have been reported to be infected with the New Jersey serotype of EHD virus (15).…”

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

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Epizootic hemorrhagic disease virus of deer: an electron microscopic study

Tsai¹,

Karstad²

1970

Can. J. Microbiol.

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A mouse brain adapted strain of epizootic hemorrhagic disease (EHD) virus of deer was studied by ultrathin section and negative contrast electron microscopy. In sections of infected BHK-21 cells and neurons of newborn mouse brains, aggregates of virus particles, some appearing to be lacking their outer coats, were observed within membrane-bound vesicles. The development of virus particles was associated with intracytoplasmic viral matrices, consisting of moderately electron-dense granules. The viral particles measured about 58.8 nm in diameter in ultrathin sections and 62.3 nm in negative contrast preparations. Tubular structures, closely associated with viral matrices, were frequently found in infected cells. In one deer intracerebrally inoculated with virulent EHD virus, viral matrices, virus particles, and tubular structures, identical with those in infected BHK-21 cells and mouse neurons, were found in the cytoplasm of endothelial cells of blood vessels. The morphological details of EHD virus are compared with those of other viruses, and the differences in structure are discussed.

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Section: Cl~allenge Experiments and Ne~itralization Testssupporting

confidence: 83%

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

“…The EHD virus has been adapted and propagated in the newborn mouse brain in several laboratories (22,30). The mouse-adapted virus rapidly loses its virulence for deer (22).…”

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

“…The mouse-adapted virus rapidly loses its virulence for deer (22). HeLa cells have been reported to be infected with the New Jersey serotype of EHD virus (15).…”

Section: Cl~allenge Experiments and Ne~itralization Testsmentioning

confidence: 99%

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Epizootic hemorrhagic disease virus of deer: an electron microscopic study

Tsai¹,

Karstad²

1970

Can. J. Microbiol.

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“…Since epizootic hemorrhagic disease (EHD) was first reported by Shope et al (25,26), the properties of the virus and viral pathogenesis in its natural host, the white-tailed deer, have been the subjects of several publications (7,14,27,28,29,30,36). The viral morphogenesis has been studied in detail in the brains of newbom mice as well as in BHK-21 cells.…”

mentioning

confidence: 99%

Ultrastructural Characterization of Genome of Epizootic Hemorrhagic Disease Virus

Tsai

Karstad

1973

Infect Immun

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Purified epizootic hemorrhagic disease (EHD) virus preparations were treated with urea and sodium perchlorate. The viral ribonucleic acid (RNA) released when spread on protein monolayers according to the Kleinschmidt technique was examined by rotary shadow-casting electron microscopy. The viral RNA released by urea treatment had filaments which frequently formed three to five loop-shaped figures of varied length. In 80% of the virus particles the lengths of the viral RNA released were 2.5 to 4.5 μm. The sodium perchlorate-released viral nucleic acid also appeared linear, and about 70% had lengths of 0.1 to 2.0 μm, the longest filament measuring 5.8 μm. Evidence was obtained that EHD virus contains double-stranded RNA as its genetic material and the molecular weight of the EHD viral RNA was calculated to be 12.2 × to 10 6 to 15.1 × 10 6 daltons.

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