The atypical Rho GTPase RhoD is a regulator of actin cytoskeleton dynamics and directed cell migration

Blom, Magdalena; Reis, Katarina; Heldin, Johan; Kreuger, Johan; Aspenström, Pontus

doi:10.1016/j.yexcr.2017.02.013

Cited by 27 publications

(22 citation statements)

References 147 publications

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“…[ [147][148][149] CHAPERONES, UPR AND ER STRESS The overexpression of XBP1 and ASF1A, together with downexpression of APC11 and PCBP1, indicate a possible increased apoptosis, induced by cell cycle regulator impaired ubiquitination, translation arrest and epigenetic instability. Additionally, the UPR activation and an attempt to correct protein folding could be favorite by the overexpression of DNAJC10.…”

Section: Retinoic Acid Cycle (Rdh5 Mkv)mentioning

confidence: 99%

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

et al. 2018

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Retinitis pigmentosa (RP) is a very heterogeneous inherited ocular disorder group characterized by progressive retinal disruption. Retinal pigment epithelium (RPE) degeneration, due to oxidative stress which arrests the metabolic support to photoreceptors, represents one of the principal causes of RP. Here, the role of oxidative stress in RP onset and progression was analyzed by a comparative whole transcriptome analysis of human RPE cells, treated with 100 µg/ml of oxLDL and untreated, at different time points. Experiment was thrice repeated and performed on Ion Proton TM sequencing system. Data analysis, including low quality reads trimming and gene expression quantification, was realized by CLC Genomics Workbench software. The whole analysis highlighted 14 clustered "macro-pathways" and many sub-pathways, classified by selection of 5271 genes showing the highest alteration of expression. Among them, 23 genes were already known to be RP causative ones (15 over-expressed and 8 down-expressed), and their enrichment and intersection analyses highlighted new 77 candidate related genes (49 over-expressed and 28 down-expressed). A final filtering analysis then highlighted 29 proposed candidate genes. This data suggests that many new genes, not yet associated with RP, could influence its etiopathogenesis.

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Section: Retinoic Acid Cycle (Rdh5 Mkv)mentioning

confidence: 99%

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

et al. 2018

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“…In a correlation plot derived from the MFA anlysis ( Figure 5(c)), the contribution of the individual parameters (the individual DMGs, phagocytosis and IL-1β) is presented. Moreover, our data also highlighted that the DMGs UBAP2L (a ubiquitin-associated protein [29]), ANKRD27 (Ankyrin-repeat domain protein [30]) and RHOD (a Rho GTPase [31,32]) were the strongest determinants in distinguishing responders from non-responders.…”

Section: Justification Of the Bcg Responder Module By Validation Agaimentioning

confidence: 81%

Identification of DNA methylation patterns predisposing for an efficient response to BCG vaccination in healthy BCG-naïve subjects

et al. 2019

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The protection against tuberculosis induced by the Bacille Calmette Guérin (BCG) vaccine is unpredictable. In our previous study, altered DNA methylation pattern in peripheral blood mononuclear cells (PBMCs) in response to BCG was observed in a subgroup of individuals, whose macrophages killed mycobacteria effectively (‘responders’). These macrophages also showed production of Interleukin-1β (IL-1β) in response to mycobacterial stimuli before vaccination. Here, we hypothesized that the propensity to respond to the BCG vaccine is reflected in the DNA methylome. We mapped the differentially methylated genes (DMGs) in PBMCs isolated from responders/non-responders at the time point before vaccination aiming to identify possible predictors of BCG responsiveness. We identified 43 DMGs and subsequent bioinformatic analyses showed that these were enriched for actin-modulating pathways, predicting differences in phagocytosis. This could be validated by experiments showing that phagocytosis of mycobacteria, which is an event preceding mycobacteria-induced IL-1β production, was strongly correlated with the DMG pattern.

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“…Although RhoD was recruited to S. Typhimurium invasion sites in a SopB-dependent manner, it did not contribute to any of the tested phenotypes. RhoD has been shown to participate in actin cytoskeleton reorganisation (Blom, Reis, Heldin, Kreuger, & Aspenstrom, 2017;Durkin et al, 2017;Gad, Nehru, Ruusala, & Aspenstrom, 2012;Koizumi et al, 2012;Nehru, Almeida, & Aspenstrom, 2013) and membrane trafficking events (Blom et al, 2015;Nehru, Voytyuk, Lennartsson, & Aspenstrom, 2013). Future studies are needed to elucidate its role in S. Typhimurium infection.…”

Section: R-ras1mentioning

confidence: 99%

Salmonellaexploits host Rho GTPase signalling pathways through the phosphatase activity of SopB

Truong

Boddy

Canadien

et al. 2018

Cellular Microbiology

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Salmonella uses Type 3 secretion systems (T3SSs) to deliver virulence factors, called effectors, into host cells during infection. The T3SS effectors promote invasion into host cells and the generation of a replicative niche. SopB is a T3SS effector that plays an important role in Salmonella pathogenesis through its lipid phosphatase activity. Here, we show that SopB mediates the recruitment of Rho GTPases (RhoB, RhoD, RhoH, and RhoJ) to bacterial invasion sites. RhoJ contributes to Salmonella invasion, and RhoB and RhoH play an important role in Akt activation. R-Ras1 also contributes to SopB-dependent Akt activation by promoting the localised production of PI(3,4)P /PI(3,4,5)P . Our studies reveal new signalling factors involved in SopB-dependent Salmonella infection.

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The atypical Rho GTPase RhoD is a regulator of actin cytoskeleton dynamics and directed cell migration

Cited by 27 publications

References 147 publications

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

Identification of DNA methylation patterns predisposing for an efficient response to BCG vaccination in healthy BCG-naïve subjects

Salmonellaexploits host Rho GTPase signalling pathways through the phosphatase activity of SopB

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