The Automated Reagin Test: Results Compared with VDRL and FTA-ABS Tests

Stevens, Roy W.; Stroebel, Ernst

doi:10.1093/ajcp/53.1.32

Am J Clin Pathol

1970

DOI: 10.1093/ajcp/53.1.32

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The Automated Reagin Test: Results Compared with VDRL and FTA-ABS Tests

Roy W. Stevens

Ernst Stroebel

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Cited by 10 publications

(2 citation statements)

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“…Originally designed for a single-channel conitinuous-flow system, it has been adapted to a multichannel system (Schroeter, Taswell, Kierland, and Sweatt, 1971). In both, the sensitivity and specificity appeared to be greater than in the manual VDRL test (McGrew, Stout, and Falcone, 1968b;Stevens and Stroebel, 1970). Until the beginning of 1974, the routine screening at the Centre National de Transfusion Sanguine (CNTS) was effected on two single-channel continuous-flow systems using a cardiolipid antigen coated on inert particlest.…”

mentioning

confidence: 99%

Automation of a flocculation test for syphilis on Groupamatic equipment.

Garretta¹,

Pâris-Hamelin²,

Gener³

et al. 1975

Sexually Transmitted Infections

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mentioning

confidence: 99%

Automation of a flocculation test for syphilis on Groupamatic equipment.

Garretta¹,

Pâris-Hamelin²,

Gener³

et al. 1975

Sexually Transmitted Infections

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“…There are several different automated tests in use in syphilis serology. These include CF procedures (6,15), a flocculation test (with antigen adsorbed to charcoal particles; references 13, 17,18), and a fluorescent treponemal antibody absorption test (G. W. Stout et al, Amer. Pub.…”

mentioning

confidence: 99%

Automation of a Hemagglutination-Inhibition Test for Parainfluenza 3 Antibodies in Bovine Sera 1

Webert¹,

Cohen²

1971

Applied Microbiology

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An automated hemagglutination-inhibition (HI) test for the "shipping fever" strain (SF-4) of parainfluenza 3 antibody in bovine sera was developed and compared to manual tube and microtiter test procedures. The automated system operating at 60 samples per hr provided the most test results per specified time period, and the manual tube test provided the least. The manual microtiter test and the automated system at 40 samples per hr, falling between the two above procedures, were comparable in the number of sera that could be titrated in 1 day by one technician. There was little difference between automated and manual test reproducibility when measured at the twofold titer one-dilution difference level. However, the automated system titrated a higher number of sera at the same titer on repeat runs than either of the manual test procedures. The automated one-quartile difference reproducibility (each twofold dilution subdivided into 4 units-"quartiles") was equal to the manual test one-dilution difference reproducibility. The standard deviation of the per cent variation from the mean of paired serum titers for 40sample-per-hr runs ranged from ±3.49 to ±5.36%. The manual and automated systems were of comparable sensitivity in their detection of negative sera.

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An automated test for rheumatoid factor

Stevens

Stroebel

Gaafar

1970

Arthritis & Rheumatism

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A continuous flow system utilizing a plasma fraction Il-charcoal suspension for the detection and quantitation of rheumatoid factor has been developed. Tests indicate that the reactivity levels of the automated procedure and those of manual latex particle technics are comparable. Interestin automated laboratory procedures prompted study of a system to detect and quantitate rheumatoid factor (RF) using a charcoal indicator in a continuous flow system. Automated Reagin (AR) equipment (1,2) and test reagents, plasma fraction I1 and standardized charcoal, were studied to provide a test of suitable reactivity. This report describes the test, illustrates the serologic sensitivity and specificity of the technic and notes advantages of the automated procedure. MATERIALS AND METHODS Latex tests.A slide macroscopic test" and a quantitative latex fixation FII-LP procedure (3) were used as standard tests for rheumatoid factor (RF) . sions were added to heated (30 min a t 56" C) serum dilutions 1:20-1:5120. After incubation for 90 min at 56" C and then overnight at 5" C, the mixtures were centrifuged and examined for agglutination.I n addition to 750 specimens selected by latex tests, the following heated sera were studied: 92 reactive in a test for antinuclear antibody (4,5), 72 reactive in VDRL and fluorescent treponemal antibody-absorption (FTA-ABS) tests (6) , and 414 "normal" premarital specimens.Desiccated human plasma fraction 11 (FII)? and standardized charcoal (C$ were obtained. FII-C suspensions were prepared in glycine buffered 0.5% saline, p H 8.2. T h e flow-through system and serum diluent, dye markers and cleaning reagent were prepared and used accGrding to AR test procedures (1).Optimum concentrations of FII and C were established by machine titrations of FII-LP reactive and nonreactive sera. T h e serologic test r e s u h reported in this paper were ohtained with FII-C suspensions containing 5 mg FII and 0.25 mg C/ml. Preparations of 10 mg FII/ml were stored overnight a t 5" C and then heated 1 hr at 63" C. Five volumes were added to 1 volume of charcoal; the mixture was stirred 30 min, and 4 volumes of glycine buffer were added. Suspensions were stored at 5" C.

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The Automated Reagin Test: Results Compared with VDRL and FTA-ABS Tests

Cited by 10 publications

References 0 publications

Automation of a flocculation test for syphilis on Groupamatic equipment.

Automation of a flocculation test for syphilis on Groupamatic equipment.

Automation of a Hemagglutination-Inhibition Test for Parainfluenza 3 Antibodies in Bovine Sera 1

An automated test for rheumatoid factor

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