The Bacillary Postbiotics, Including 2-Undecanone, Suppress the Virulence of Pathogenic Microorganisms

Abstract

Secreted molecules from probiotic Bacilli have often been considered potential pharmaceuticals to fight infections caused by bacterial or yeast pathogens. In the present study, we investigated the antagonistic potential of secreted probiotic filtrates (hereafter, postbiotics) derived from Lactobacillus plantarum cells against pathogenic microorganisms, such as Escherichia coli, Staphylococcus aureus, and Candida albicans. We found that the postbiotics mitigate the biofilms of the tested pathogens with no notable effect on their planktonic growth. In addition, the postbiotics suppressed some virulence traits, for instance, the dendrite swarming motility of E. coli and yeast-to-hyphal switch in C. albicans. Further assays with an active constituent produced by the L. plantarum cells–2-undecanone revealed two significant findings: (i) 2-undecanone inhibits C. albicans biofilms and hyphae in vitro and in a Caenorhabditis elegans model, and (ii) it interacts specifically with Gln 58 amino acid residue of hyphal wall protein-1 (Hwp-1) in molecular docking analysis. The results suggest the targeted mode of antagonistic action of 2-undecanone against C. albicans biofilm. In total, the findings of the study depict an appealing strategy to use postbiotics, including specific ketone molecules, produced by L. plantarum for developing novel antibiofilm and anti-hyphal pharmaceuticals.

Nigrospora oryzae Causing Leaf Spot Disease on Chrysanthemum × morifolium Ramat and Screening of Its Potential Antagonistic Bacteria

“…2-Nonanol and 2-Nonanone can inhibit Fusarium graminearum growth and subspore germination, and prevent wheat scab caused by Fusarium graminearum [ 56 , 57 ]. 2-Undecanone has a targeted mode of antagonistic action against Candida albicans biofilm [ 58 ]. Fermentation broth produced by Bacillus amyloliquefaciens containing 2-Heptanone, 2-Nonanone, 2-Nonanol, 2-Undecanone, and 2-Undecanol has good antagonistic effects against plant pathogenic fungi such as Monilinia fructigena , Glomerella cingulate , Rhizoctonia solani , and so on [ 59 ].…”

Effect of Eurotium cristatum on Saccharification Efficiency, Microorganisms in Mold Culture, and Liquor Flavor during Wuliangye Liquor Production

“…[ 7 ] Analyzing the series of changes in starch molecules during saccharification helps elucidate how E. cristatum affects saccharification efficiency. E. cristatum in a mold culture also influences the original microorganisms [ 8 ] that further contribute to the flavor of Wuliangye liquor. [ 9 ] Therefore, the effects of E. cristatum on saccharification efficiency, microorganisms in mold culture, and flavor of Wuliangye liquor should be comprehensively analyzed to estimate the feasibility of using E. cristatum in the production of Wuliangye liquor.…”