The BAP Module: A Multisignal Integrator Orchestrating Growth

Bouré, Nathalie; Kumar, S. Vinod; Arnaud, Nicolas

doi:10.1016/j.tplants.2019.04.002

Cited by 24 publications

(28 citation statements)

References 82 publications

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“…We also observed that multiple transcription factors belonging to the BAP module (i.e. BZR/ARF/PIF families) (Bouré et al, 2019) were upregulated in SALK_015201 as compared to the WT consistent with the elongated hypocotyl phenotype. Classic negative regulators of photomorphogenesis such as BBX28, bHLH transcription factors PIF4, PIF3, PIL1 and PIL6 (Lin et al, 2018; Huq and Quail, 2002; Dong et al, 2017; Luo et al, 2014; Fujimori et al, 2004) showed an increased transcript abundance in SALK_015201 as compared to the WT (Figure 3 and Supplemental Table 1).…”

Section: Resultsmentioning

confidence: 58%

“…Transcription factors belonging to BZR, ARF and PIF families, otherwise known as the BAP module, work synergistically to promote the transcription of genes such as IAA19, SAUR15 and PRE1 , that are involved in positively regulating hypocotyl growth (Bouré et al, 2019). Our transcriptomic data shows that these downstream growth regulators are collectively elevated in the slim shady allele, which can explain the elongated hypocotyl phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…The central signaling network that positively regulates hypocotyl growth in Arabidopsis consists of three major families of interdependently working transcription factors-BRASSINAZOLE RESISTANT1 (BZR1), AUXIN RESPONSE FACTORs (ARFs) and the PHYTOCHROME INTERACTING FACTORs (PIFs), collectively referred to as the BZR/ARF/PIF module or the ‘BAP module’ (Bouré et al, 2019). The BAP module works in concert to activate downstream genes that promote cell elongation in the hypocotyl (Favero, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Analysis of whole genome sequence data available for this SALK line (Hu et al, 2019) identified a single base pair deletion within the coding sequence of PHYB. Transcriptomic comparison of SALK_015201 and wild type seedlings with and without auxin determined that genes associated with the BAP regulatory module (Bouré et al, 2019) were altered in this T-DNA background. Genetic complementation analyses confirmed the presence of a new phyb allele, which we call slim shady, in SALK_015201.…”

Section: Introductionmentioning

confidence: 99%

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slim shadyis a novel allele of PHYTOCHROME B present in the T-DNA line SALK_015201

Dash

McEwan²,

Montes

et al. 2021

Preprint

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Auxin is a hormone that is required for hypocotyl elongation during seedling development. In response to auxin rapid changes in transcript and protein abundance occur in hypocotyls and some auxin responsive gene expression is linked to hypocotyl growth. To functionally validate proteomic studies, a reverse genetics screen was performed on mutants in auxin-regulated proteins to identify novel regulators of plant growth. This uncovered a long hypocotyl mutant, which we called slim shady, in an annotated insertion line in IMMUNOREGULATORY RNA-BINDING PROTEIN (IRR). Overexpression of the IRR gene failed to rescue the slim shady phenotype and characterization of a second T-DNA allele of IRR found that it had a wild-type hypocotyl length. The slim shady mutant has an elevated expression of numerous genes associated with the brassinosteroid-auxin-phytochrome (BAP) regulatory module compared to wild-type, including transcription factors that regulate brassinosteroid, auxin and phytochrome pathways. Additionally, slim shady seedlings fail to exhibit a strong transcriptional response to auxin. Using whole genome sequence and transcriptomics data for SALK_015201C we determined that a novel single nucleotide polymorphism in PHYTOCHROME B was responsible for the slim shady phenotype. This is predicted to induce a frameshift and premature stop codon at leucine 1125, within the histidine kinase-related domain of the carboxy terminus of PHYB, which is required for phytochrome signaling and function. Genetic complementation analyses with phyb-9 confirmed that slim shady is a mutant allele of PHYB. This study advances our understanding of the molecular mechanisms in seedling development, by furthering our understanding of how light signaling is linked to auxin dependent cell elongation. Furthermore, this study highlights the importance of confirming the genetic identity of research material before attributing phenotypes to known mutations sourced from T-DNA stocks.

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Section: Resultsmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

slim shadyis a novel allele of PHYTOCHROME B present in the T-DNA line SALK_015201

Dash

McEwan²,

Montes

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Hence, CUC2 is likely to be a substrate of IBR5 in leaf serration development. Auxin response factors (ARFs) were provided to be phosphorylated and dephosphorylated, and also involved in cell expansion [44,45], which suggests that they may act as substrates of IBR5 in leaf development.…”

Section: Discussionmentioning

confidence: 99%

IBR5 Regulates Leaf Serrations Development via Modulation of the Expression of PIN1

Kong

Huang

Xiong

et al. 2019

IJMS

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Biodiversity in plant shape is mainly attributable to the diversity of leaf shape, which is largely determined by the transient morphogenetic activity of the leaf margin that creates leaf serrations. However, the precise mechanism underlying the establishment of this morphogenetic capacity remains poorly understood. We report here that INDOLE-3-BUTYRIC ACID RESPONSE 5 (IBR5), a dual-specificity phosphatase, is a key component of leaf-serration regulatory machinery. Loss-of-function mutants of IBR5 exhibited pronounced serrations due to increased cell area. IBR5 was localized in the nucleus of leaf epidermis and petiole cells. Introducing a C129S mutation within the highly conserved VxVHCx2GxSRSx5AYLM motif of IBR5 rendered it unable to rescue the leaf-serration defects of the ibr5-3 mutant. In addition, auxin reporters revealed that the distribution of auxin maxima was expanded ectopically in ibr5-3. Furthermore, we found that the distribution of PIN1 on the plasma membrane of the epidermal and cells around the leaf vein was compromised in ibr5-3. We concluded that IBR5 is essential for the establishment of PIN-FORMED 1 (PIN1)-directed auxin maxima at the tips of leaf serration, which is vital for the elaborated regulation during its formation.

show abstract

slim shady is a novel allele of PHYTOCHROME B present in the T‐DNA line SALK_015201

Dash

McEwan²,

Montes

et al. 2021

Plant Direct

View full text Add to dashboard Cite

Auxin is a hormone that is required for hypocotyl elongation during seedling development. In response to auxin, rapid changes in transcript and protein abundance occur in hypocotyls, and some auxin responsive gene expression is linked to hypocotyl growth. To functionally validate proteomic studies, a reverse genetics screen was performed on mutants in auxin‐regulated proteins to identify novel regulators of plant growth. This uncovered a long hypocotyl mutant, which we called slim shady , in an annotated insertion line in IMMUNOREGULATORY RNA‐BINDING PROTEIN ( IRR ). Overexpression of the IRR gene failed to rescue the slim shady phenotype and characterization of a second T‐DNA allele of IRR found that it had a wild‐type (WT) hypocotyl length. The slim shady mutant has an elevated expression of numerous genes associated with the brassinosteroid‐auxin‐phytochrome (BAP) regulatory module compared to WT, including transcription factors that regulate brassinosteroid, auxin, and phytochrome pathways. Additionally, slim shady seedlings fail to exhibit a strong transcriptional response to auxin. Using whole genome sequence data and genetic complementation analysis with SALK_015201C, we determined that a novel single nucleotide polymorphism in PHYTOCHROME B was responsible for the slim shady phenotype. This is predicted to induce a frameshift and premature stop codon at leucine 1125, within the histidine kinase‐related domain of the carboxy terminus of PHYB, which is required for phytochrome signaling and function. Genetic complementation analyses with phyb‐9 confirmed that slim shady is a mutant allele of PHYB . This study advances our understanding of the molecular mechanisms in seedling development, by furthering our understanding of how light signaling is linked to auxin‐dependent cell elongation. Furthermore, this study highlights the importance of confirming the genetic identity of research material before attributing phenotypes to known mutations sourced from T‐DNA stocks.

show abstract

The BAP Module: A Multisignal Integrator Orchestrating Growth

Cited by 24 publications

References 82 publications

slim shadyis a novel allele of PHYTOCHROME B present in the T-DNA line SALK_015201

slim shadyis a novel allele of PHYTOCHROME B present in the T-DNA line SALK_015201

IBR5 Regulates Leaf Serrations Development via Modulation of the Expression of PIN1

slim shady is a novel allele of PHYTOCHROME B present in the T‐DNA line SALK_015201

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