The Behavior and Significance of Degradative Plasmids Belonging to Inc Groups in &lt;i&gt;Pseudomonas&lt;/i&gt; within Natural Environments and Microcosms

Shintani, Masaki; Takahashi, Yurika; Yamane, Hisakazu; Nojiri, Hideaki

doi:10.1264/jsme2.me10155

Cited by 44 publications

(46 citation statements)

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“…Considering that many fully sequenced genomes of Escherichia, Shigella, Klebsiella, and Enterobacter have lacI-homologous genes (data not shown), the number of transconjugants of these genera belonging to the family Enterobacteriaceae could be underestimated. A similar bias may explain the absence of detected transconjugants of NAH7 in the family Enterobacteriaceae in this study (Table 2), even though IncP-9 plasmids are known to be transferred to and be replicated in the hosts of this family (13,53,54). Delftia was not detected in the soil bacterial community analyses, suggesting that Delftia was also a minor component of the soil bacteria.…”

Section: Discussionmentioning

confidence: 52%

“…To date, Pseudomonas, a member of Gammaproteobacteria, has been considered the major host of IncP-7 plasmids, and these plasmids have not been able to be replicated in recipients belonging to the Betaproteobacteria (8,13,20,42). Indeed, no member of the Betaproteobacteria was obtained as a transconjugant of pCAR1::gfp by using the culture-dependent method in this study.…”

Section: Discussionmentioning

confidence: 89%

“…These Inc groups are classified based on a Pseudomonas strain as a recipient, and IncP-1 corresponds to IncP in the Escherichia coli plasmid classification (12). These groups contain major catabolic plasmids whose behaviors in the natural environment have been extensively investigated (13), and thus, they were used as model plasmids. Their genes involved in conjugative transfer, the mobility (MOB) and mating pair formation (MPF) genes, were classified into MOB P , MOB H , and MOB F classes and MPF T , MPF F , and MPF T types, respectively (14).…”

mentioning

confidence: 99%

“…Their genes involved in conjugative transfer, the mobility (MOB) and mating pair formation (MPF) genes, were classified into MOB P , MOB H , and MOB F classes and MPF T , MPF F , and MPF T types, respectively (14). The host ranges of the three plasmids have been previously documented as being broad (pBP136), narrow (pCAR1), and intermediate (NAH7) (13). A broad-host-range plasmid is proposed to be able to replicate itself and be maintained stably in at least two different bacterial classes without selection, whereas a narrow-host-range plasmid is in the same class or genus (13).…”

mentioning

confidence: 99%

“…The host ranges of the three plasmids have been previously documented as being broad (pBP136), narrow (pCAR1), and intermediate (NAH7) (13). A broad-host-range plasmid is proposed to be able to replicate itself and be maintained stably in at least two different bacterial classes without selection, whereas a narrow-host-range plasmid is in the same class or genus (13).…”

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confidence: 99%

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Single-Cell Analyses Revealed Transfer Ranges of IncP-1, IncP-7, and IncP-9 Plasmids in a Soil Bacterial Community

Shintani

Matsui

Inoue

et al. 2014

Appl Environ Microbiol

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The conjugative transfer ranges of three different plasmids of the incompatibility groups IncP-1 (pBP136), IncP-7 (pCAR1), and IncP-9 (NAH7) were investigated in soil bacterial communities by culture-dependent and culture-independent methods. Pseudomonas putida, a donor of each plasmid, was mated with soil bacteria, and green fluorescent protein (GFP), encoded on the plasmid, was used as a reporter protein for successful transfer. GFP-expressing transconjugants were detected and separated at the single-cell level by flow cytometry. Each cell was then analyzed by PCR and sequencing of its 16S rRNA gene following either whole-genome amplification or cultivation. A large number of bacteria within the phylum Proteobacteria was identified as transconjugants for pBP136 by both culture-dependent and culture-independent methods. Transconjugants belonging to the phyla Actinobacteria, Bacteroidetes, and Firmicutes were detected only by the culture-independent method. Members of the genus Pseudomonas (class Gammaproteobacteria) were identified as major transconjugants of pCAR1 and NAH7 by both methods, whereas Delftia species (class Betaproteobacteria) were detected only by the culture-independent method. The transconjugants represented a minority of the soil bacteria. Although pCAR1-containing Delftia strains could not be cultivated after a one-to-one filter mating assay between the donor and cultivable Delftia strains as recipients, fluorescence in situ hybridization detected pCAR1-containing Delftia cells, suggesting that Delftia was a "transient" host of pCAR1. P lasmids are circular or linear extrachromosomal replicons, which are often transmissible by conjugation (1, 2). Plasmids can spread among bacteria effectively and act as key "vehicles" of pathogenicity and environmentally relevant traits (1). Therefore, the conjugative transfer of plasmids is one of the most important mechanisms to promote the rapid evolution and adaptation of bacteria. Knowledge about the host range of a plasmid is essential to the understanding of how the plasmid is transferred in different environments. A plasmid host is generally recognized in two ways: (i) cells can obtain the plasmid by conjugative transfer and (ii) cells can replicate and maintain the plasmid after cell division. Although some plasmids are known to make junctions to cross the kingdom barrier (3), to date, plasmid host ranges have been determined by using culture-dependent methods by detecting colonies of transconjugants on selective media. This method is unable to detect uncultivated or noncultivable hosts and is limited by both the transfer system and its replication and maintenance systems. Recently, several studies have shown that noncultivable bacteria carry plasmids (4, 5), suggesting that other uncultivated or noncultivable bacteria could also have plasmids. Because the majority of environmental bacteria are difficult to cultivate, determining an accurate host range for the plasmid transfer function (i.e., a transfer range of plasmids) has not yet been accomplishe...

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Section: Discussionmentioning

confidence: 52%