The behaviour of LDH-3 in patients with malignant diseases during therapy with cytostatic drugs and prednisone, studied by LDH-isoenzyme electrophoresis on cellulose acetate

Abstract

SummaryA modified method used for the quantitative estimation of LDH-isoenzymes in serum after electrophoresis on c~llulose acetate is described.Total LDH-activit~ and isoenzyme distribution in serum samples of capillary blood are compared to those in samples collected by venipuncture.Total LDH-values and Lt)H-distributions both in 2c' normal children and 14 children with maii~.o~.-,t ~_ise,~es aze given.:~'nen studying the LDH<tlstribution in serum samples of children with malignant diseases a significantly decreased LDH-3 was found in many patients. LDH-4 and LDH-5 were also reduced. It was noted that these children all received therapy with prednisone. Possible explanations are discussed.