The beneficial effect of genetically engineered Schwann cells with enhanced motility in peripheral nerve regeneration: review

Gravvanis, Andreas; Lavdas, Alexandros A.; Papalois, Apostolos; Tsoutsos, Dimosthenis; Matsas, Rebecca

doi:10.1007/978-3-211-72958-8_11

Cited by 22 publications

(12 citation statements)

References 37 publications

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“…Viral introduction of vertebrate PST or STX gene into cultured SCs has proven effective in augmenting their reparative efficacy after transplantation into the lesioned spinal cord (21)(22)(23)(24) and has shown promise in improving stem cell-based therapies (18). The cellular basis for these treatments involves cell migration and axon outgrowth, which as shown here can also be augmented by the PSA produced by PST Nm .…”

Section: Discussionmentioning

confidence: 78%

“…For example, overexpression of the polysialyltransferase gene in vivo promotes regeneration of lesioned axons and recruitment of neural progenitors into lesions (17)(18)(19)(20). Similarly, introduction of the gene into cells in culture has improved cell grafting therapies using Schwann cells in spinal cord injury (21)(22)(23)(24) or stem cell-derived progenitors (for review, see Ref. 18).…”

mentioning

confidence: 99%

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Enzymatic Engineering of Polysialic Acid on Cells in Vitro and in Vivo Using a Purified Bacterial Polysialyltransferase

Maarouf

Yaw

Lindhout

et al. 2012

Journal of Biological Chemistry

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Background: Polysialic acid (PSA) promotes neuroplasticity, and overexpression of polysialyltransferase genes augments brain repair. Results: Extracellular application of a purified bacterial polysialyltransferase (PST Nm ) produces PSA on vertebrate cells in vitro and in vivo, and the product is biologically active. Conclusion: Polysialylation of cells by PST Nm is rapid, persistent, but not permanent. Significance: A PST Nm -based approach may provide an alternative to polysialyltransferase gene therapy.

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Section: Discussionmentioning

confidence: 78%

mentioning

confidence: 99%

Enzymatic Engineering of Polysialic Acid on Cells in Vitro and in Vivo Using a Purified Bacterial Polysialyltransferase

Maarouf

Yaw

Lindhout

et al. 2012

Journal of Biological Chemistry

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“…Schwann cell-based therapies that use transplantation techniques for the treatment of nerve tissue repairing are being widely investigated for their potential as clinical applications. [42][43][44] LIPUS applied in conjunction with other forms of biologic stimulation is worth considering when optimizing an innovative ''multilevel'' form of treatment. Second, application of LIPUS in vivo is likely to be considered to stimulate repair of damaged peripheral nerve tissues.…”

Section: Discussionmentioning

confidence: 99%

Effect of low‐intensity pulsed ultrasound on the expression of neurotrophin‐3 and brain‐derived neurotrophic factor in cultured Schwann cells

et al. 2009

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It is generally known that low-intensity pulsed ultrasound (LIPUS) accelerates peripheral nerve tissue regeneration. However, the precise cellular mechanism involved is still unclear. The purpose of this study was to determine how the Schwann cells respond directly to LIPUS stimuli. Thus, we investigated the effect of LIPUS on cell proliferation, neurotrophin-3 (NT-3), and brain-derived neurotrophic factor (BDNF) mRNA expression in rat Schwann cells. Schwann cells were enzymatically isolated from postnatal 1-3 day rat sciatic nerve tissue and cultured in the six-well plate. The ultrasound was applied at a frequency of 1 MHz and an intensity of 100 mW/cm(2) spatial average temporal average for 5 minutes/day. The control group was cultured in the same way but without the administration of ultrasound. Immunohistochemistry demonstrated that more than 98% of the experimental and control cells were positive for S-100, NT-3, and BDNF. With 5-bromo-2'-deoxyuridine (BrdU) assay, the stimulated cells also exhibited an increase in the rate of cell proliferation on days 4, 7, 10, and 14. Further investigation found that mRNA expression of NT-3 was significantly upregulated in experimental groups compared with the control 14 days after the LIPUS stimulation (the ratio of NT-3/beta-actin was 0.56 +/- 0.13 vs. 0.41 +/- 0.09, P < 0.01), whereas the mRNA expression of BDNF was significantly downregulated in experimental groups compared with the control (the ratio of BDNF/beta-actin was 0.51 +/- 0.05 vs. 0.60 +/- 0.08, P < 0.05). These results demonstrated that the application of LIPUS promotes cell proliferation and NT-3 gene expression in Schwann cells, and involved in the alteration of BDNF gene expression.

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“…First, given its nontoxicity and a wide margin of biologic safety, it may be used as an effective physical stimulant when engineering peripheral nerve tissue. Schwann cell-based therapies that use transplantation techniques for the treatment of nerve tissue repairing are being widely investigated for their www.intechopen.com potential as clinical applications (Rochkind, Astachov et al 2004;Li, Ping et al 2006;Gravvanis, Lavdas et al 2007). LIPUS applied in conjunction with other forms of biologic stimulation, is worth considering when optimizing an innovative "multi-level" form of treatment.…”

Section: Lipus Enhances Neurite Elongation In Rat Cortical Neuronsmentioning

confidence: 99%

Effect of Low-Intensity Pulsed Ultrasound on Nerve Repair

Li¹,

Zhang²,

Ren³

2012

Tissue Regeneration - From Basic Biology to Clinical Application

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The beneficial effect of genetically engineered Schwann cells with enhanced motility in peripheral nerve regeneration: review

Cited by 22 publications

References 37 publications

Enzymatic Engineering of Polysialic Acid on Cells in Vitro and in Vivo Using a Purified Bacterial Polysialyltransferase

Enzymatic Engineering of Polysialic Acid on Cells in Vitro and in Vivo Using a Purified Bacterial Polysialyltransferase

Effect of low‐intensity pulsed ultrasound on the expression of neurotrophin‐3 and brain‐derived neurotrophic factor in cultured Schwann cells

Effect of Low-Intensity Pulsed Ultrasound on Nerve Repair

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