The Beneficial Effects of Renal Transplantation on Altered Oxidative Status of ESRD Patients

Abstract

Renal transplantation (RT), has been considered the best therapeutic option for end stage renal disease (ESRD). Objective. To determine the effect of RT on the evolution of oxidative DNA status. Methods. Prospective cohort (N = 50 receptors of RT); genotoxic damage, 8-hydroxy-2′-deoxyguanosine (8-OHdG), and DNA repair enzyme, human 8-oxoguanine-DNA-N- glycosylase-1 (hOGG1); and antioxidants, superoxide dismutase (SOD) and glutathione peroxidase (GPx), were evaluated. Results. Before RT, 8-OHdG were significantly elevated (11.04 ± 0.90 versus 4.73 ± 0.34 ng/mL) compared to healthy controls (p = 0.001), with normalization after 6 months of 4.78 ± 0.34 ng/mL (p < 0.001). The same phenomenon was observed with hOGG1 enzyme before RT with 2.14 ± 0.36 ng/mL (p = 0.01) and decreased significantly at the end of the study to 1.20 ng/mL (p < 0.001) but was higher than controls, 0.51 ± 0.07 ng/mL (p < 0.03). Antioxidant SOD was elevated at 24.09 ± 1.6 IU/mL versus healthy controls (p = 0.001) before RT; however, 6 months after RT it decreased significantly to 16.9 ± 1.6 IU/mL (p = 0.002), without achieving the levels of healthy controls (p = 0.01). The GPx, before RT, was significantly diminished with 24.09 ± 1.6 IU/mL versus healthy controls (39.0 ± 1.58) (p = 0.01), while, in the final results, levels increased significantly to 30.38 ± 3.16 IU/mL (p = 0.001). Discussion. Patients with ESRD have important oxidative damage before RT. The RT significantly reduces oxidative damage and partially regulates the antioxidant enzymes (SOD and GPx).

The Behavior of the Type of Peritoneal Transport in the Inflammatory and Oxidative Status in Adults Under Peritoneal Dialysis

“…We previously evaluated the long-term behavior of hOGG-1 in patients with renal transplantation and we found low levels of hOGG-1 in HC compared with patients in HD or DP before transplantation. However, these measures were made with no distinction of type of PT or renal replacement therapy (38).…”

Oxidative Stress in Kidney Diseases: The Cause or the Consequence?

“…Such an effect has been reported in X-ray cross-complementing group 1 repair enzyme, where Arg399Gln polymorphism conferred the increased risk of development of end-stage renal disease (ESRD) (Trabulus et al 2012 ). Also, measuring the presence and/or the activity of DNA-repair enzymes, such as 8-oxoguanine-DNA- N -glycosylase-1 (Cerrillos-Gutierrez et al 2016 ), might help monitoring the altered OS conditions in renal patients.…”

Vascular Calcification and Oxidative DNA Damage as Nontraditional Cardiovascular Risk Factors in Chronic Renal Disease

“…When performing the correlation tests between oxidative stress and MBD results in patients with CKD, we found that SOD were correlated negatively with the 8-0HdG marker and the VC score and the oxidative stress correlated positively with alkaline phosphatase and phosphorus. The 8-OHdG correlated negatively with VC, alkaline phosphatase, and P. Based on the findings, the importance of the association between oxidative stress and VC in patients with ESRD, and the recent studies evidencing the persistence of alterations in oxidative status even after TR, it is necessary to evaluate the usefulness of antioxidant therapeutic strategies in this population of patients [46]. The results of this study, being the first in our country to describe the association between oxidative stress behavior and VC, contribute to the future evaluation of the oxidative status of the ESRD for prognostic and therapeutic purposes.…”