The Beneficiary of bone marrow-derived mesenchymal stem cells primed with oestradiol in alleviating collagen-induced arthritis​

Abstract

This study was conducted to investigate the effects of the oestradiol (ES)pulsed bone marrow-derived mesenchymal stem cell (BM-MSC) to treat adjuvant-induced arthritis in Wistar rats. BM-MSCs were pulsed with ES (0, 10,100 and 1000 nM) for 24 hours. RA induced collagen and Freund's Complete Adjuvant into the base of the tail of Wistar rats. In vitro results showed that the least effective concentration of ES that can promote potent anti-inflammatory properties in the MSC population is 100 nM. At this concentration, ES increases the inhibition of the poly-clonal T lymphocyte proliferation, production of IDO, IL-10, Nitric oxide, TGF-β, and expression of CXCR4 and CCR2 mRNA in the MSC population. Based on In vitro results, the RA rats were treated with 2×106 MSCs or ES-pulsed MSCs (100nM) on day 10 when all animals had developed signs of RA. The in vivo results showed that the treatment with ES-pulsed BM-MSCs reduced the severity of the RA more profoundly than treatment with BM-MScs alone. The ability of ES-pulsed BM-MSCs to reduce symptoms and RA markers like CRP, RF, and nitric oxide was comparable to that of prednisolone. Prednisolone was more successful in reducing inflammatory cytokines than treatment with ES-pulsed BM-MSCs. ES-pulsed BM-MSCs were more successful in increasing anti-inflammatory cytokines than treatment with Prednisolone. The ability of ES-pulsed BM-MSCs to decrease the level of nitric oxide was comparable to that of prednisolone. Collectively, ES-pulsed BM-MSCs may be a helpful strategy in controlling RA.

Impact of 17β-Estradiol on the Shape, Survival, Osteogenic Transformation, and mRNA Expression of Gingiva-Derived Stem Cell Spheroids

“…In another study, the dosages of 17β-estradiol used were 10, 50, 100, 500, 1, 10, and 50 mM [47]. The least effective concentration of 17β-estradiol that could promote potent anti-inflammatory properties in the mesenchymal stem cell population was 100 nM [48]. Lower concentrations (0.01 and 1 nM) appeared to promote RUNX2 expression, but higher quantities of the drug (1 and 10 nM) decreased the expression.…”

Therapeutic potential of Coptis chinensis for arthritis with underlying mechanisms

“…Studies have shown that CC can treat a variety of arthritis, and it is better for OA, RA and GA. CC has the characteristics of multiple components, multiple targets, and multiple pathways in the treatment of arthritis. Currently, IL-10, IL-1β, IL-6, MMP-3, TNF-α and other targets have been verified to play a role in treating arthritis by CC ( Table 2 ) ( Sun et al, 2011 ; Yuan et al, 2014 ; Kienhorst et al, 2015 ; Huang et al, 2019 ; Thomas, 2020 ; Che et al, 2021 ; Li et al, 2021 ; Wu et al, 2021 ; Arra et al, 2022 ; Zhang et al, 2022 ; Cao et al, 2022 ; Du et al, 2022 ; Pacifici, 2022 ; Wirth et al, 2022 ; Xiao et al, 2022 ; Yang et al, 2022 ; Sun et al, 2023a ; Barbarroja et al, 2023 ; Li et al, 2023 ; Brix et al, 2023 ; Wang et al, 2023b ; Wang et al, 2023 ; Ghosh et al, 2023 ; González-Chávez et al, 2023 ; Hecquet et al, 2023 ; Jahantigh et al, 2023 ; Ji et al, 2023 ; La Bella et al, 2023 ; Leung et al, 2023 ; Lin et al, 2023 ; Luo et al, 2023 ; Zeng et al, 2023 ; Zhang et al, 2023 ). However, the types of arthritis treated with these targets are not completely clear.…”