The benefits of voluntary physical exercise after traumatic brain injury on rat's object recognition memory: A comparison of different temporal schedules

Amorós-Aguilar, Laura; Portell-Cortès, Isabel; David, Cristhian; Torras-Garcia, Meritxell; Riubugent-Camps, Èlia; Almolda, Beatriz; Coll-Andreu, Margalida

doi:10.1016/j.expneurol.2020.113178

Cited by 15 publications

(10 citation statements)

References 48 publications

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“…After 7 days VWR, besides improvement of motor coordination, the reduction of anxiety was successfully achieved. Although previous study demonstrated that voluntary exercise facilitates neuroplasticity after TBI when it occurs during a delayed time window [24][25][26], there was other experiment showed the exercise improved rat memory when it was commenced soon and later after injury [25]. Our results further con rmed that it is possible to use voluntary exercise at early stage (2 days after TBI) to ease the anxiety behavior of TBI mice.…”

Section: Discussionsupporting

confidence: 70%

“…Voluntary exercise, as one of the essential rehabilitative intervention, has been proved that could attenuate neuronal loss, reduce neuroin ammation, improve mood and then facilitate recovery after TBI [21][22][23]. Existed data showed that voluntary exercise or voluntary exercise pre-conditioning facilitated neuroplasticity after TBI, through reducing the level of in ammatory factors and elevating the level of brain-derived neurotrophic factor (BDNF) [24][25][26][27][28]. However, there were some other experiments showed opposite effects, depending on the time windows [24][25][26]29].…”

Section: Introductionmentioning

confidence: 99%

“…Existed data showed that voluntary exercise or voluntary exercise pre-conditioning facilitated neuroplasticity after TBI, through reducing the level of in ammatory factors and elevating the level of brain-derived neurotrophic factor (BDNF) [24][25][26][27][28]. However, there were some other experiments showed opposite effects, depending on the time windows [24][25][26]29].…”

Section: Introductionmentioning

confidence: 99%

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Voluntary exercise attenuates mice anxiety after traumatic brain injury via inhibiting NLRP3 inflammasome activation

et al. 2022

Preprint

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Background: Psychological disorders, particularly the anxiety after traumatic brain injury (TBI) have received great attention both in clinical practice and experimental investigation. New therapeutic strategies are urgently required nowadays to improve mental condition of TBI patients. Neuroinflammation is the typical adaptive response that played important roles in psychiatric illness after brain tissue damage. Therefore, the immune-targeted therapies has aroused attention. Voluntary exercise has been considered as the most essential rehabilitative intervention following TBI. However, there were some experiments showed opposite effects, which varies with different time windows. Whether and when the voluntary exercise should be taken after TBI remain to be defined. The aim of the current study was to elucidate whether in-time voluntary exercise could improve the neurological recovery and reduce the anxiety of TBI mice by inhibiting the neuroinflammation via attenuating the activation of NLRP3 inflammasome.Methods: Kunming mice (8 weeks) were subjected to TBI and randomly divided into 4 groups One week voluntary wheel running (VWR) was applied to TBI mice soon after trauma - at 2nd day after injury. The neurofunction of TBI mice was detected by neurological severity score (NSS) and beam walking assay. Anxiety behavior was evaluated by open-field (OF) test and elevated plus maze (EPM) test. The cellular inflammatory signal, including IL-12, IFN-γ, CCL2, IL-10 and TGF-β, as well as the activation of microglia and NLRP3 inflammasome in the perilesional cortex were investigated.Results: After one week VWR, neurological function recovery was enhanced while the anxiety behavior of TBI mice was significantly eased. Accompanied with this, anti-inflammatory signaling, IL-10 in particular, greatly elevated. NLRP3 expression, caspase-1 activation and pro-inflammatory cytokines IL-1β/ IL-18 secretion were significantly inhibited. All these alterations were consistent with reduced microglia activation in the perilesional site and positively correlated with the weaken of anxiety behavior.Conclusion: In-time VWR could be a high potential therapeutic strategy for neuropsychiatric disorders following TBI by targeting neuroinflammation.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Voluntary exercise attenuates mice anxiety after traumatic brain injury via inhibiting NLRP3 inflammasome activation

et al. 2022

Preprint

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“…A major advantage of the task is that it can be used in both rats and mice (Agarwal et al, 2020;Chang et al, 2020). Current iterations of the NOR paradigm are widely used to study memory (Cole et al, 2020), synaptic plasticity (Lee et al, 2020), impairment and/or recovery of function in brain disease (Grayson et al, 2015), TBI (Amoros-Aguilar et al, 2020), stress (Brivio et al, 2020;Glushchak et al, 2021), aging (Amirazodi et al, 2020), sleep (Shahveisi et al, 2020), autism (Batista et al, 2019;Gandhi and Lee, 2020), and epigenetics (Ellis et al, 2020;Sadat-Shirazi et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Homecage-Based Novel Object Recognition Task for Rodents

Wooden

Spinetta

Nguyen

et al. 2021

Front. Behav. Neurosci.

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The recognition of novel objects is a common cognitive test for rodents, but current paradigms have limitations, such as low sensitivity, possible odor confounds and stress due to being performed outside of the homecage. We have developed a paradigm that takes place in the homecage and utilizes four stimuli per trial, to increase sensitivity. Odor confounds are eliminated because stimuli consist of inexpensive, machined wooden beads purchased in bulk, so each experimental animal has its own set of stimuli. This paradigm consists of three steps. In Step 1, the sampling phase, animals freely explore familiar objects (FO). Novel Objects (NO1 and NO2) are soiled with bedding from the homecage, to acquire odor cues identical to those of the FO. Steps 2 and 3 are test phases. Herein we report results of this paradigm from neurologically intact adult rats and mice of both sexes. Identical procedures were used for both species, except that the stimuli used for the mice were smaller. As expected in Step 2 (NO1 test phase), male and female rats and mice explored NO1 significantly more than FO. In Step 3 (NO2 test phase), rats of both sexes demonstrated a preference for NO2, while this was seen only in female mice. These results indicate robust novelty recognition during Steps 2 and 3 in rats. In mice, this was reliably seen only in Step 2, indicating that Step 3 was difficult for them under the given parameters. This paradigm provides flexibility in that length of the sampling phase, and the delay between test and sampling phases can be adjusted, to tailor task difficulty to the model being tested. In sum, this novel object recognition test is simple to perform, requires no expensive supplies or equipment, is conducted in the homecage (reducing stress), eliminates odor confounds, utilizes 4 stimuli to increase sensitivity, can be performed in both rats and mice, and is highly flexible, as sampling phase and the delay between steps can be adjusted to tailor task difficulty. Collectively, these results indicate that this paradigm can be used to quantify novel object recognition across sex and species.

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“…Voluntary exercise has been proven to attenuate neuronal loss, inhibit glia activation, reduce neuroinflammation, improve mood, and subsequently facilitate recovery after TBI [ 32 , 33 , 34 , 35 ]. Existing data showed that voluntary exercise or voluntary exercise pre-conditioning facilitated neuroplasticity after TBI via the reduction in the level of inflammatory factors and elevation in the level of brain-derived neurotrophic factor [ 36 , 37 , 38 , 39 , 40 ]. However, other studies showed contradictory effects, depending on the different exercise strategy [ 36 , 37 , 38 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Voluntary Exercise to Reduce Anxiety Behaviour in Traumatic Brain Injury Shown to Alleviate Inflammatory Brain Response in Mice

et al. 2023

IJMS

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Traumatic brain injury is a leading cause of neuroinflammation and anxiety disorders in young adults. Immune-targeted therapies have garnered attention for the amelioration of TBI-induced anxiety. A previous study has indicated that voluntary exercise intervention following TBI could reduce neuroinflammation. It is essential to determine the effects of voluntary exercise after TBI on anxiety via inhibiting neuroinflammatory response. Mice were randomly divided into four groups (sham, TBI, sham + voluntary wheel running (VWR), and TBI + VWR). One-week VWR was carried out on the 2nd day after trauma. The neurofunction of TBI mice was assessed. Following VWR, anxiety behavior was evaluated, and neuroinflammatory responses in the perilesional cortex were investigated. Results showed that after one week of VWR, neurofunctional recovery was enhanced, while the anxiety behavior of TBI mice was significantly alleviated. The level of pro-inflammatory factors decreased, and the level of anti-inflammatory factors elevated. Activation of nucleotide oligomerization domain-like thermal receptor protein domain associated protein 3 (NLRP3) inflammasome was inhibited significantly. All these alterations were consistent with reduced microglial activation at the perilesional site and positively correlated with the amelioration of anxiety behavior. This suggested that timely rehabilitative exercise could be a useful therapeutic strategy for anxiety resulting from TBI by targeting neuroinflammation.

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The benefits of voluntary physical exercise after traumatic brain injury on rat's object recognition memory: A comparison of different temporal schedules

Cited by 15 publications

References 48 publications

Voluntary exercise attenuates mice anxiety after traumatic brain injury via inhibiting NLRP3 inflammasome activation

Voluntary exercise attenuates mice anxiety after traumatic brain injury via inhibiting NLRP3 inflammasome activation

A Sensitive Homecage-Based Novel Object Recognition Task for Rodents

Voluntary Exercise to Reduce Anxiety Behaviour in Traumatic Brain Injury Shown to Alleviate Inflammatory Brain Response in Mice

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